A kind of recombinant sodium vibrio and its application

Abstract

The invention relates to the field of biotechnology, in particular to a recombinant natrigovibrio and application thereof. The invention provides a recombinant sodium-demanding Vibrio, which expresses glycerol dehydratase, glycerol dehydratase activator and alcohol dehydrogenase, and the recombinant sodium-demanding Vibrio is comparable to wild-type sodium-demanding Vibrio have reduced expression and / or enzymatic activity of transcriptional regulators, said transcriptional regulators being arcA and / or glpR. The present invention transforms Natovibrio so that it can efficiently utilize glycerol to ferment and produce 1,3-propanediol, the yield of 1,3-Propanediol is high, the by-products are few, and the extraction and separation process is simple; The potential biosafety problems of traditional 1,3-propanediol production strains and the low tolerance of strains to salt in crude glycerol have important industrial application value.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a recombinant natrigovibrio and application thereof. Background technique

[0002] 1,3-Propanediol is an important chemical raw material, which can be used as an organic solvent in ink, printing and dyeing, paint, lubricant, antifreeze and other industries. Its main use is as a monomer for the synthesis of polyester and polyurethane. Especially polymerized with terephthalic acid to form polytrimethylene terephthalate (PTT). Compared with PET (polyethylene terephthalate) and PBT (polybutylene terephthalate), PTT has better properties, such as better pollution resistance, toughness and resilience, and Ultraviolet properties, etc., in addition, it also has the advantages of wear resistance, low water absorption, and low static electricity. Therefore, PTT is considered as an upgraded product of PET and has broad market prospects.

[0003] At present, the production method of 1,3-propa...