36results about How to "Avoid Biosecurity Concerns" patented technology

The invention provides a nano-silver antibacterial fabric and a preparation method thereof. The nano-silver antibacterial fabric has the characteristics that a zinc oxide nano line grows on the fabric; and silver nano particles are deposited on the zinc oxide nano line. The zinc oxide nano line is synthesized by a solution chemical method; and the silver nano particles are deposited on the zinc oxide nano line by a simple 'immersion-illumination' method. The nano-silver antibacterial fabric prepared by the method has extremely obvious long-time antibacterial performance; the silver nano particles are small in size and cannot be accumulated; a synthesis process is environment-friendly; and the nano-silver antibacterial fabric can be recycled and reused. The nano-silver antibacterial fabric can be used for single-point water treatment to reduce microorganism content in water and can be suitable for medical surgical dressing and medical fabrics; and the nano-silver antibacterial fabric can be used as an antibacterial air filtration device for preventing bio-aerosol from being gathered on a ventilating, heating and air conditioning system.

The invention discloses a serum-free culture medium for human umbilical cord MSCs (mesenchymal stem cells). The serum-free culture medium comprises the major ingredients of amino acid, inorganic salt ingredients, growth factors and albumin ingredients. The serum-free culture medium for human umbilical cord MSCs does not contain any serum ingredients; in addition, the ingredients are clear; the foreign protein pollution and pathogenic microorganism risks are avoided; the biosecurity problem of the MSCs clinic application is solved. When the serum-free culture medium provided by the invention is used for culturing the human umbilical cord MSCs, the high-quantity high-purity safe and high-quality umbilical cord MSCs can be obtained in vitro; meanwhile, the cell survival rate can be improved; the stem cell characteristics are maintained. The problem of insufficient cell quantity can be solved; the cell number, the survival rate and the immunophenotyping in each batch are more stable; the relevant index requirements are met.

The invention relates to a preparation method of a subcutaneous filling material, in particular to a preparation method of a subcutaneous filling hyaluronic acid gel, and belongs to the technical field of preparation of biological materials. According to the preparation method disclosed by the invention, an aldehyde group and an hydrazide group are introduced to a high molecular chain, so that theeffect of fine-needle injection and rapid in-situ forming is realized, and the problem of needle burst caused for the fact that a pre-formed hydrogel is high in viscosity and fine needle injection isnot easy to perform and biological safety caused by residue of a small-molecular cross-linking agent are avoided. In addition, the preparation method disclosed by the invention adopts a process of modifying aldehyde-based sodium hyaluronate by a maleic acyl group after freeze-drying, and the high-substitution-degree grafting of the maleic acyl group on a hyaluronic acid molecular chain is realized, so that hyaluronic acid can be further polymerized under the irradiation of ultraviolet light in the subsequent in-situ molding process, the bonding strength of the gel on tissues of an injection part is greatly improved, and the problem that a gel filler is displaced when being extruded by external force is completely overcome.

The invention discloses a preparation method of novel renewable hydrogel with low bacterial adhesion and sterilization and regenerable functions and belongs to the technical field of hydrogel preparation. The preparation method specifically comprises steps as follows: 1) a UV polymerization reaction; 2) an esterification reaction; 3) an ATRP reaction; 4) a sliver-supporting reaction. The preparation method is simple and feasible, a polymerization method guarantees the effectiveness and controllability of the length of a functional polymer brush, and the long-time antibacterial effect is realized through slow release of silver ions. The product has a remarkable inhibition effect on escherichia coli and staphylococcus aureus; compared with an antibacterial product containing single components in the prior art, the product has greatly improved antibacterial efficiency. Besides, through the environmental responsibility of the functional polymer brush, shrinkage and extension of the polymerbrush due to the outside environment are changed, desorption of dead bacteria is further driven, and the regenerable antibacterial property of the hydrogel is realized.

The invention discloses a biodegradable Mg-Ca-Mn-Sn magnesium alloy material and a preparation method and application thereof. The biodegradable Mg-Ca-Mn-Sn magnesium alloy material is prepared from the following components, by mass percentage, 0.5-3% of Ca, 0.1-2% of Mn, 1-5% of Sn and the balance Mg and inevitable impurity elements brought in by raw materials. The specific preparation method includes smelting of the raw materials, casting molding, uniform annealing treatment and hot extrusion processing. The biodegradable Mg-Ca-Mn-Sn magnesium alloy has no any toxicity on human bodies and is good in mechanical performance, the preparation method is simple and low in cost, plate-shaped and rod-shaped materials in different size and cross section requirements can be prepared as required, and the biodegradable Mg-Ca-Mn-Sn magnesium alloy material can be used for preparation of bone lamellae, bone nails and other orthopedic implants, cardiovascular stents and other medical materials.

The invention relates to a method for inducing the differentiation of mesenchymal stem cells of human embryo livers into islet beta-like cells and stably expressing insulin and special induced liquid thereof. The method comprises the following steps: separating, purifying and amplifying mesenchymal stem cells of human embryo livers; then taking out cells formed after the third generation and inducing the cells by the special induced liquid to obtain islet beta-like cell mass capable of expressing the insulin stably. The induced liquid can be obtained by adding TAT-PDX1 fusion protein in cell culture fluid. The method can realize in-vitro induction of mesenchymal stem cells of human embryo livers into islet beta-like cells capable of secreting the insulin stably. When transplanted in the body of a diabetic patient, the obtained islet beta-like cells can further adjust blood sugar level so as to realize cell therapy of diabetes. Therefore, research in the field has broad clinic application prospect and can bring greater economic benefit and social benefit.

The invention discloses a biodegradable Zn-Mg-Zr alloy material. The biodegradable Zn-Mg-Zr alloy material is characterized in that the Zn-Mg-Zr alloy material comprises, by mass, 0.0001-10% of Mg, 0.0001-5% of Zr and the balance Zn. The alloy material is low in cost, nontoxic, excellent in mechanical property, low in degradation rate and excellent in biocompatibility. The invention further discloses a preparation method of the biodegradable Zn-Mg-Zr alloy material. The method is easy to implement and low in cost, plate-shaped and rod-shaped materials with different size and cross section requirements can be prepared as required, and the method can be used for preparation of bone lamellas, bone nails and other bone implantation materials as well as cardiovascular stents and other medical materials.

The invention provides a microbial sample scribing system and method and solves the problems that in the prior art the microbial sample scribing treatment is low in biosecurity, incapable of standardizing the operation, highly random in manual operation, high in human cost and low in efficiency. The microbial sample scribing system comprises a communication unit, a mechanical unit and a circuit, wherein the communication unit is in signal connection with the mechanical unit; the communication unit and the mechanical unit are respectively and electrically connected with the externally connected circuit. The microbial sample scribing system and method disclosed by the invention enable the biosecurity problem to be solved, protect the experimental environment and the operating personnel, are standardized in the operation, reduce the human cost, improve the working efficiency, standardize and mechanize the operation procedures, and increase the repeatability of the experimental result.

The invention discloses a serum-free medium for large-scale culture of human umbilical cord mesenchymal stem cells. The medium mainly includes amino acid, an inorganic salt component, a growth factorand an albumin component. The serum-free medium does not contain any serum component, has clear components, overcomes the danger of heterologous protein contamination and pathogenic microorganisms, and solves the biosafety problem of MSCs clinical application. The human umbilical cord mesenchymal stem cells can be cultured invitro in large scale by using the serum-free medium. The umbilical cord mesenchymal stem cells with high volume, high purity, safety and high quality can be obtained. At the same time, the cell survival rate can be improved, and the characteristics of stem cells can be maintained. The serum-free medium can solve the problem of insufficient cell number, the cell number, the survival rate and the immunophenotype of each batch are more stable, and the requirements for relevant indexes can be met.

The invention discloses a method for preparing a CD163 gene edited pig by using a single base editor SpRY-BE4. By editing a CD163 gene of an isolated fibroblast genome of a pig, a 228th basic group ofan E3 exon of the CD163 diallelic gene is subjected to site-specific mutagenesis from C to T, and a TGA terminator is formed after mutagenesis, so that the expression of the E3 exon is terminated inadvance, and a CD163 diallelic gene mutant cell is obtained; and the nucleotide sequence of the E3 exon is the sequence 2. The CD163 diallelic gene knockout pig obtained by the invention has the capability of resisting PRRSV (porcine reproductive and respiratory syndrome virus), which shows that the pig resisting PRRSV can be obtained by the method for preparing the CD163 diallelic gene knockout pig.

The invention relates to an antibacterial modification method of polymer materials. The antibacterial modification method comprises following steps: preparing diazomethane with a thioether group; using diazomethane to carry out chemical modification on the surface of a polymer material; and carrying out Ag-S coordination reactions between a nano silver particle solution and the chemically modifiedpolymer material. After antibacterial modification, Ag is bonded with the polymer material through chemical bonds, so during the using process, silver leakage is prevented, and the biological safetyis guaranteed.

The invention discloses a biodegradable Mg-Ca-Mn-Sn magnesium alloy material and a preparation method and application thereof. The biodegradable Mg-Ca-Mn-Sn magnesium alloy material is prepared from the following components, by mass percentage, 0.5-3% of Ca, 0.1-2% of Mn, 1-5% of Sn and the balance Mg and inevitable impurity elements brought in by raw materials. The specific preparation method includes smelting of the raw materials, casting molding, uniform annealing treatment and hot extrusion processing. The biodegradable Mg-Ca-Mn-Sn magnesium alloy has no any toxicity on human bodies and is good in mechanical performance, the preparation method is simple and low in cost, plate-shaped and rod-shaped materials in different size and cross section requirements can be prepared as required, and the biodegradable Mg-Ca-Mn-Sn magnesium alloy material can be used for preparation of bone lamellae, bone nails and other orthopedic implants, cardiovascular stents and other medical materials.

The invention provides a microbial sample scribing system and method and solves the problems that in the prior art the microbial sample scribing treatment is low in biosecurity, incapable of standardizing the operation, highly random in manual operation, high in human cost and low in efficiency. The microbial sample scribing system comprises a communication unit, a mechanical unit and a circuit, wherein the communication unit is in signal connection with the mechanical unit; the communication unit and the mechanical unit are respectively and electrically connected with the externally connected circuit. The microbial sample scribing system and method disclosed by the invention enable the biosecurity problem to be solved, protect the experimental environment and the operating personnel, are standardized in the operation, reduce the human cost, improve the working efficiency, standardize and mechanize the operation procedures, and increase the repeatability of the experimental result.

The invention relates to the technical field of biology, in particular to recombinant vibrio natriegens and application thereof. The invention provides the recombinant vibrio natriegens which expresses glycerol dehydratase, a glycerol dehydratase activator and alcohol dehydrogenase, and compared with wild type vibrio natriegens, the recombinant vibrio natriegens has the advantages that the expression and / or enzyme activity of a transcription regulation factor is reduced, and the transcription regulation factor is arcA and / or glpR. According to the method, vibrio natriegens is modified, so that glycerol fermentation can be efficiently utilized to produce 1, 3-propylene glycol, the yield of 1, 3-propylene glycol is high, byproducts are few, and the extraction and separation process is simple; and moreover, the vibrio natriegens is used for solving the potential biosafety problem of the traditional 1, 3-propylene glycol production strain and the problem of low tolerance of the strain to salt in crude glycerine, and has important industrial application value.

A method for preparing a biodegradable magnesium alloy with high corrosion resistance, which belongs to the field of material technology and is carried out according to the following steps: (1) Prepare metal magnesium, metal zinc, metal silver and metal calcium as raw materials; (2) In N2+SF6 Under the protection of mixed gas, heat the metal magnesium to 760±5℃, add metal silver, metal calcium and metal zinc in sequence after melting, and stir until evenly mixed; (3) Cool down to 740±5℃, add argon gas, and heat at 740 Keep at ±5°C for 10~20min; (4) Cool to 720±5°C for casting; (5) Cover with graphite powder, heat to 300~320°C and keep for 2~4 hours, raise the temperature to 350~450°C and keep for 8~ 12h, water cooling; (6) Keep at 250~350℃ for 25~35min, and then plastically deform at high temperature; (7) Perform multiple normal temperature deformation-intermediate annealing, and finally air cool to normal temperature. The method of the present invention enables the magnesium alloy to obtain high corrosion resistance and good mechanical properties through single-phase and fine-grain treatment, and can be used for the preparation of different implanted devices.

The invention discloses a serum-free culture medium for CHO cells. The main components include amino acids, inorganic salt components, vitamins, trace elements, yeast hydrolyzate, albumin and the like. The CHO cell serum-free medium of the present invention does not contain any serum components and animal-derived components, and its affinity is increased compared with other serum-free mediums. Cells can be directly inoculated into this serum-free medium from serum medium or other serum-free medium, and grow normally, eliminating the tedious domestication process. The invention improves the growth density and viability of CHO cells, increases the tolerance of cells in them, prolongs the maintenance time of the growth platform, and most importantly improves the ability of CHO engineered cells to express foreign proteins in them, greatly improving Product yield.

The invention relates to an antibacterial modification method of polymer materials. The antibacterial modification method comprises following steps: preparing diazomethane with a thioether group; using diazomethane to carry out chemical modification on the surface of a polymer material; and carrying out Ag-S coordination reactions between a nano silver particle solution and the chemically modifiedpolymer material. After antibacterial modification, Ag is bonded with the polymer material through chemical bonds, so during the using process, silver leakage is prevented, and the biological safetyis guaranteed.

The invention discloses a method for preparing cows with precise BLG-gene knockout by using a third-generation base editor. The method for preparing the cows with gene knockout includes the followingsteps: mutating the 277th base from the 5' end of a BLG gene in a starting cow genome into T from C, wherein the BLG gene is a DNA molecule shown in SEQ ID No. 1. A large-animal point mutation technology is established, precise single-base mutation is performed, termination codons are through exquisite design of C-T mutation so as to terminate protein translation in advance, so that the purpose ofgene knockout is achieved. Natural mutant animals are simulated, genomic double-strand break is not caused by the BE3-based point mutation technology when the method is compared with a traditional gene editing technology, and thus random mutation and even damage to the genome are avoided greatly, so that the method is safer. Important technical support is provided for researches of precise gene editing and gene knockout of cows.

The invention provides a first small molecule peptide with oxygen carrying potential, a second small molecule peptide capable of generating active oxygen, and a preparation method of the first and second small molecule peptides. The sequence of the first small molecule peptide with oxygen carrying potential of the present invention is C17H31-CONH-VRGDS-COOH. The unsaturated double bond functionalgroup in the structure can be oxidized by lipoxygenase to form a conjugated double bond and the side groups generate hydroperoxide. The method of constructing the second small molecule peptide by thefirst small molecule peptide is an enzymatic method, and the structure of the second small molecule peptide is C17H31O2-CONH-VRGDS-COOH. The method that the second small-molecule peptide generates active oxygen is a Fenton-like reaction. C17H31O2-CONH-VRGDS-COOH produces cytotoxic active oxygen under the catalysis of Fe<2+>.

The invention relates to a composite nano vaccine for tumor treatment and a preparation method thereof. The preparation method comprises the following steps that firstly metal sulfide-antigen nano particles are prepared, and then the metal sulfide-antigen nano particles and immature dendritic cells are co-incubated for 2-6 hours to obtain the metal sulfide-antigen-DC composite nano vaccine. According to the composite nano vaccine for tumor treatment and the preparation method thereof, an antigen with high biocompatibility is used as a template and a raw material to synthesize the nano particles, the preparation method is simple and green, and the problem of biosafety when the nano particles are applied to living bodies can be solved; the size of the synthesized metal sulfide nano particles is 10 + / -2.1 nm, and the metal sulfide nano particles are uniform in size, high in stability and high in bioavailability; the synthesized metal sulfide nano particles can be used as a metal adjuvant to stimulate bone marrow cells to induce maturation of immature DC cells, increase antigen presentation and activate downstream T cell related adaptive immune response; the synthesized composite nano vaccine has a high antigen loading rate, and can realize co-delivery of the metal adjuvant and the antigen.

The invention relates to a method for preparing a subcutaneous filling, in particular to a method for preparing a hyaluronic acid gel for subcutaneous filling, and belongs to the technical field of biological material preparation. In the preparation method of the present invention, the method of introducing aldehyde groups and hydrazide groups into the polymer chain is adopted to realize the effect of fine needle injection and rapid in-situ molding, and avoid the explosion caused by the high viscosity of the preformed hydrogel and the difficulty of fine needle injection. Needle issues and biosafety issues caused by small molecule crosslinker residues. Moreover, in the preparation method of the present invention, the process of modifying aldehyde sodium hyaluronate with maleoyl groups after freeze-drying is used to realize the grafting of maleoyl groups with a high degree of substitution on the molecular chain of hyaluronic acid, so that hyaluronic acid can be used in subsequent raw materials. In the process of in-situ molding, further polymerization can occur under the irradiation of ultraviolet light, which greatly improves the adhesive strength of the gel to the tissue at the injection site, and completely overcomes the problem of the gel filler moving and shifting under external force extrusion.

The invention discloses a method for preparing cows with precise BLG-gene knockout by using a third-generation base editor. The method for preparing the cows with gene knockout includes the followingsteps: mutating the 277th base from the 5' end of a BLG gene in a starting cow genome into T from C, wherein the BLG gene is a DNA molecule shown in SEQ ID No. 1. A large-animal point mutation technology is established, precise single-base mutation is performed, termination codons are through exquisite design of C-T mutation so as to terminate protein translation in advance, so that the purpose ofgene knockout is achieved. Natural mutant animals are simulated, genomic double-strand break is not caused by the BE3-based point mutation technology when the method is compared with a traditional gene editing technology, and thus random mutation and even damage to the genome are avoided greatly, so that the method is safer. Important technical support is provided for researches of precise gene editing and gene knockout of cows.

The invention relates to the technical field of microbial fertilizers, in particular to a bacillus cereus strain, a lettuce microbial fertilizer and a preparation method and application of the bacillus cereus strain and the lettuce microbial fertilizer. The invention provides a strain of bacillus cereus, wherein the Latin name of the bacillus cereus is bacillus cereus; the preservation number is GDMCC No: 62282. According to the invention, a strain of bacillus cereus is separated from solanum nigrum stems, and metabolites and inactivated bacteria generated by the bacillus cereus can significantly promote the growth of the leaf lettuce and improve the quality of the leaf lettuce.

The invention relates to the field of biotechnology, in particular to a recombinant natrigovibrio and application thereof. The invention provides a recombinant sodium-demanding Vibrio, which expresses glycerol dehydratase, glycerol dehydratase activator and alcohol dehydrogenase, and the recombinant sodium-demanding Vibrio is comparable to wild-type sodium-demanding Vibrio have reduced expression and / or enzymatic activity of transcriptional regulators, said transcriptional regulators being arcA and / or glpR. The present invention transforms Natovibrio so that it can efficiently utilize glycerol to ferment and produce 1,3-propanediol, the yield of 1,3-Propanediol is high, the by-products are few, and the extraction and separation process is simple; The potential biosafety problems of traditional 1,3-propanediol production strains and the low tolerance of strains to salt in crude glycerol have important industrial application value.

To solve the problems of manual ruling in the prior art that the biological safety is low, the operation is non-standard, the randomness of manual operation is large, the labor cost is high, the efficiency is low, and a culture medium is easy to scratch, the invention provides a gravity self-adaptive ruling mechanism which comprises a fixing end, a locking sleeve, a connecting end and a ruling pen, wherein the fixing end adopts a rigid structure; the top of the fixing end is adaptive to an external mechanical moving mechanism; the top of the locking sleeve adopts an upwards opening sleeve structure, and is fixed to the bottom of the fixing end in a coordinated manner; the bottom of the locking sleeve adopts a downwards opening sleeve structure; a platform bulging in the inner diameter direction is arranged at the bottom of the locking sleeve; a positioning block of which the section is T-shaped is arranged at the top of the connecting end; the top of the connecting end is in positioning coordination with the platform; the bottom of the locking sleeve is also in interference fit with the rod part of the connecting end; an opening is formed in the top of the ruling pen, and tightly clamps the bottom of the connecting end. The work efficiency is high, the operating process is standardized and mechanized, and the culture medium is prevented from being scratched.

The invention discloses a plant transgenic selection system using betaine aldehyde dehydrogenase (BADH) as a marker gene and use thereof and belongs to the technical field of gene engineering. In the system, BADH is used as a marker gene of an exogenous gene and NaCl is used as a selecting agent. The use comprises the following steps: (1) determining NaCl concentration which can just inhibit plant tissue differentiation; (2) transferring the exogenous gene which contains the BADH marker gene into a plant cell or tissue; and (3) culturing the transgenic tissue or cell into which the BADH marker gene is transferred on a culture medium in which the NaCl concentration determined by the step (1), obtaining the transgenic tissue or cell into which the BADH marker gene is transferred into if the transgenic tissue or cell can grow, and confirming by molecular detection. The invention has the advantages of greatly lowering the cost investment in a transgenic test, solving the safety problem of transgenic organisms, and providing low cost selection for the construction of a safe and effective selection system for plant genetic transformation.

The invention relates to a method for isolating and expanding stem cells after umbilical cord tissue resuscitation and a special culture medium thereof, including the steps of umbilical cord tissue resuscitation, tissue block acquisition, isolation of primary stem cells, and expansion and passage of stem cells. Type II Collagenase, TrypLE TM Sodium chloride injection of express and prolinephthalein amino acids, the medium used is sodium selenate, bovine transferrin, ornithine, 5-7mg / ml lectin, α-tocopheryl succinate monoester , IL‑6, LIF and HGF in DMEM / F12 medium. The isolation and culture method provided by the present invention has short crawling time of human umbilical cord mesenchymal stem cells, short culture period of primary stem cells, high purity of stem cells and good activity, and can be passed down for many times. Potential for high differentiation.

The invention discloses a biodegradable Zn-Mg-Zr alloy material. The biodegradable Zn-Mg-Zr alloy material is characterized in that the Zn-Mg-Zr alloy material comprises, by mass, 0.0001-10% of Mg, 0.0001-5% of Zr and the balance Zn. The alloy material is low in cost, nontoxic, excellent in mechanical property, low in degradation rate and excellent in biocompatibility. The invention further discloses a preparation method of the biodegradable Zn-Mg-Zr alloy material. The method is easy to implement and low in cost, plate-shaped and rod-shaped materials with different size and cross section requirements can be prepared as required, and the method can be used for preparation of bone lamellas, bone nails and other bone implantation materials as well as cardiovascular stents and other medical materials.