284 results about "Osteoblast cell" patented technology

This application describes apparatuses and methods for musculoskeletal tissue engineering. Specifically, graft collar and scaffold apparatuses are provided for promoting fixation of musculoskeletal soft tissue to bone.This application provides for graft collars comprising biopolymer mesh and / or polymer-fiber mesh for fixing tendon to bone. In one aspect, the graft collar comprises more than one region, wherein the regions can comprise different materials configured to promote integration of and the regeneration of the interfacial region between tendon and bone.This application also provides for scaffold apparatuses and methods for fixing musculoskeletal soft tissue to bone. The scaffold apparatus is multiphasic, preferably triphasic, and each phase is configured promote growth and proliferation of a different cell and its associated tissue. In one aspect, the scaffold apparatus is triphasic, with phases comprising materials to promote growth and proliferation of fibroblasts, chondroblasts, and osteoblasts. In addition, an apparatus comprising two portions, each of said portion being the scaffold apparatus described above is provided, wherein each of said portion encases one end of a soft tissue graft. Further, a triphasic interference screw is provided.This application further provides apparatuses and methods for inducing formation of fibrocartilage comprising wrapping a graft collar with polymer-fiber mesh configured to apply compression to the graft collar. In another aspect, the polymer-fiber is applied directly to the graft to apply compression to the graft.

Three-dimensional tissue-like organization of cells by high cell-seeding-density culture termed as macromass culture is described. By macromass culture, cells can be made to organize themselves into a tissue-like form without the aid of a scaffold and three-dimensional macroscopic tissue-like constructs can be made wholly from cells. Tissue-like organization and macroscopic tissue-like constructs can be generated from fibroblastic cells of mesenchymal origin (at least), which can be either differentiated cells or multipotent adult stem cells. In this work, tissue-like organization and macroscopic tissue-like constructs have been generated from dermal fibroblasts, adipose stromal cells-derived osteogenic cells, chondrocytes, and from osteoblasts. The factor causing macroscopic tissue formation is large scale culture at high cell seeding density per unit area or three-dimensional space, that is, macromass culture done on a large scale. No scaffold or extraneous matrix is used for tissue generation, the tissues are of completely cellular origin. No other agents (except high cell-seeding-density) that aid in tissue formation such as tissue-inducing chemicals, tissue-inducing growth factors, substratum with special properties, rotational culture, etc, are employed for tissue formation. These tissue-like masses have the potential for use as tissue replacements in the human body. Tissue-like organization by high cell-seeding-density macromass culture can also be generated at the microscopic level.

The invention relates to a 3D (three-dimensional) uniform porous scaffold material and a preparing method of the 3D uniform porous scaffold material. The existing artificial bone scaffold material has the defects that the porosity is low, and the cell migration and the new tissue formation are limited; the micropore diameter distribution is nonuniform, and the condition is unfavorable for the distribution of osteoblast cells and the like on the surface of the material; and the pore diameter is greater, and the condition is unfavorable for the cell adhesion and cell proliferation. According to the preparing method, collagen is dissolved in a sodium carbonate solution; nanometer hydroxyapatite is added; the mixture is subpackaged into a mold; the mold is subjected to freezing and vacuum drying; a cross-linking reaction is carried out on the frozen and dried sample in an alkaline cross-linking environment; a cross-linking reaction is carried out on the cross-linked sample in an acid environment; the obtained sample is washed; and the washed sample is subjected to vacuum freeze drying, sterilization and packaging. The 3D uniform porous scaffold material and the preparing method have the advantages that the collagen and the nano-hydroxyapatite are used as raw materials, and carbon dioxide produced by reaction between sodium carbonate and alkali and ice crystal sublimation carried out in the vacuum freeze drying process are utilized, so that a micropore structure is obtained with uniform pore diameter distribution and with a pore diameter and porosity suitable for cell adhesion, proliferation and migration, and a uniform porous scaffold obtained through preparation has certain pressure resistance intensity and has a good microstructure and good biocompatibility.

The invention relates to an application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases. The application is characterized in that adipose-derived mesenchymal stem cells (Ad-MSCs) separated and extracted from autologous adipose tissues of the patient are injected to the diseased articular cavity in a large dose once. The adopted mesenchymal stem cells have immunophenotyping and representing biological marks of Ad-MSC through flow cytometry authentication, and can be directionally induced and differentiated to osteoblast and chondrocyte. Primary cells are directly injected to the articular cavity of the diseased joint to treat various joint diseases. With the adoption of the autologous adipose-derived mesenchymal stem cells as the primary cells, the cells have better differentiative potential and the ability of inhibiting inflammation and immunoregulation ability. The adipose-derived mesenchymal stem cell transplant provides a novel effective means for treating osteoarticular diseases. The method is simple and feasible, and the quantity of cells collected is great and the trauma is small. The autologous mesenchymal stem cells of the patient are applied without invading related ethics, so that the risk of immunological rejection, infectious diseases and the like is avoided.

The invention discloses a bone repair material as well as a preparation method and application thereof. The bone repair material comprises the following components: magnesium oxide, monocalcium phosphate, sodium dihydrogen phosphate and calcium-deficient hydroxyapatite. The molar ratio of the magnesium oxide to the monocalcium phosphate to a sodium dihydrogen phosphate bone cement powder is 6:1:4,5:1:3 or 3:1:1, and the calcium-deficient hydroxyapatite accounts for 20% of the total mass. A curing liquid of the bone cement is an EGCG aqueous solution, and the concentration is 120 [mu]mol / L. Aloaded drug is deferoxamine. The preparation method of the bone repair material is simple and feasible; and the setting time is suitable, the bone cement has good biocompatibility, osteogenesis and degradability, and can promote adhesion, proliferation and differentiation of osteoblasts, stimulate cell growth and stimulate differentiation of the osteoblasts into the osteoblasts, and the bone repair material can be directly injected into a bone defect part.

The invention provides methods and compositions for differentiating stromal cells from adipose tissue into cells having osteoblastic properties, and methods for improving a subject's bone structure. The methods comprise culturing stromal cells from adipose tissue in β-glycerophosphate and ascorbic acid and / or ascorbate-2-phosphate for a time sufficient to allow differentiation of said cells into osteoblasts. Such methods and compositions are useful in the production of osteoblasts for autologous transplantation into bone at a surgical site or injury. The compositions comprise adipose stromal cells, a medium capable of supporting the growth of fibroblasts and amounts of β-glycerophosphate and ascorbic acid and / or ascorbic-2 phosphate sufficient to induce the differentiation of said stromal cells into osteoblasts. The invention further provides methods of identifying compounds that affect osteoblast differentiation. Such compounds are useful in the study of bone development and in the treatment of bone disorders, including bone fractures and osteoporosis.

The present invention relates to an injectable bone regeneration material containing a bone formation enhancing peptide, and more particularly, to an injectable bone regeneration material, in which a bone formation enhancing peptide essentially containing one and more amino acid sequences among SEQ ID NO: 1 to SEQ ID NO: 28 is bonded or mixed to a gel-forming base material selected from the group consisting of chitosan, alginic acid, silk fibroin, propylene glycol, propylene glycol alginic acid, poloxamer, chondroitin sulphate, and the combination thereof. The injectable bone regeneration material according to the present invention can increase differentiation of bone marrow stromal cells and osteoblasts into bone tissue, thus maximizing tissue regeneration by a peptide capable of promoting differentiation of bone tissue and periodontal tissue regeneration. The injectable bone regeneration material is in the form of a gel, and thus can be applied to a surface of various medical devices such as implant etc., and can be mixed with bone graft particles to apply, so that it can increase a treatment effect of existing medical devices to maximize a tissue regeneration effect.