55 results about "Osteoblast cell" patented technology

The invention discloses a special-shaped titanium alloy capable of enhancing bone regeneration and repair efficacy as well as a preparation method and application of the special-shaped titanium alloy. The special-shaped titanium alloy comprises a special-shaped titanium alloy base body and a porous structure attached to the special-shaped titanium alloy base body, and the porous structure is in multi-stage size distribution and comprises long-strip-shaped gullies (100-150 micrometers wide) and large holes (30-50 micrometers wide). And the morphology in the gullies shows coralline ligaments and a nest-shaped pore channel (0.5-2 microns) structure. This can provide space and sites for the growth and adsorption of osteoblasts. According to the method, a gas phase alloying (VPA) and VPD synergistic process is adopted in a breakthrough mode, a non-oxidation / nitridation coralline-shaped hierarchical pore structure is constructed on the surface of the commercial titanium implant, and it can be achieved through verification of an aging animal model that a special-shaped matrix is uniformly modified in the whole domain, the regeneration function of aging stem cells is activated, and the bone repair and regeneration effect is effectively improved.

The invention relates to the technical field of biomedical materials, and discloses an active composite membrane with an asymmetric double-layer structure as well as a preparation method and application of the active composite membrane. The active composite membrane with the asymmetric double-layer structure is prepared from the following preparation raw materials in percentage by mass: 95 to 98 percent of chitosan; 0.5%-2% of a plant polyphenol cross-linking agent; and 1%-4% of magnesium-based degradable inorganic filler. The active composite membrane has an integrated double-layer structure without interface bonding, the compact layer can effectively block soft tissues, and the porous layer is beneficial to cell adhesion and nutrition delivery; in a wet state, the material is excellent in mechanical property, controllable in degradation and high in cell safety, can effectively inhibit bacteria and promote osteoblast proliferation and differentiation, and can meet the performance requirements of bone defect repair on the material; the preparation method adopts the steps of solvent volatilization, freeze drying and precooled alkaline alcohol solution washing for preparation, the whole preparation process is carried out in a water phase, the conditions are mild, the steps are simple, complex equipment is not needed, and extremely high clinical transformation and industrialization potential is shown.

The invention discloses a bionic bone marrow microenvironment assembly for hematopoietic stem cell in-vitro amplification and a preparation method. The bone marrow microenvironment assembly comprises a three-dimensional scaffold and osteoblasts (OBs), wherein the three-dimensional scaffold comprises chitosan (CS) and sodium alginate (Alg) which are prepared into mixed gel, and the mixed gel is freeze-dried to prepare the three-dimensional scaffold; wherein the osteoblasts (OBs) are obtained by culturing and induced differentiation of umbilical cord mesenchymal stem cells (MSCs) inoculated on a three-dimensional scaffold, and the osteoblasts (OBs) as stromal cells and the three-dimensional scaffold jointly form the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly; in the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly, the preparation of the three-dimensional scaffold comprises the processes of freezing, remelting, freezing, crosslinking, freeze-drying and the like, so that a three-dimensional space form with wrinkles on the pore surface and pore size distribution between 70 microns and 400 microns is generated in the three-dimensional scaffold, and the three-dimensional space form is closer to a real stem cell niche; therefore, the practical application effect of the bionic bone marrow microenvironment assembly for in-vitro amplification of the hematopoietic stem cells is further improved.

The application discloses a preparation method of egg yolk phospholipid polypeptide which is helpful for bone growth and improvement of brain health, and comprises the following steps: (1) raw material pretreatment to obtain a reaction substrate solution; (2) primary enzymolysis to prepare a primary enzymolysis solution; (3) secondary enzymolysis to prepare a secondary enzymolysis solution; (4) inactivation, the temperature of the secondary enzymolysis solution prepared in the step (3) is adjusted to 85-95 DEG C, and the enzyme is completely inactivated by keeping for 10-15 min; (5) fermentation; (6) post-treatment, the fermentation liquid after the fermentation is kept at 50-60 DEG C for 10-30 min, and then cooling, centrifugation, collection of supernatant, vacuum freeze drying or spray drying of the supernatant are carried out, so that the egg yolk phospholipid polypeptide powder is obtained. The natural compound rich in specific functional peptide segments and phospholipids is prepared through the two-step enzymolysis coupling fermentation process, can effectively promote the growth and development of the bone, significantly improve the learning, memory and cognitive ability, and can significantly promote the proliferation of osteoblasts.

Embodiments of the disclosure include methods and compositions related to modulation of bone using particular fibroblasts. The modulation includes reducing osteoclast activity and / or activation and / or stimulating osteoblast activity. In particular cases, bone is modulated in an individual with a bone condition, such as osteoporosis. Particular fibroblasts may be delivered to reduce inflammatory cytokine production including RANK ligand.

The utility model provides a fibrous membrane for guiding periodontal tissue regeneration, and relates to the technical field of medical instruments. Preparing a PLGA double-layer fiber membrane loaded with drug-loaded sodium hyaluronate particles through the cooperative work of electrostatic spinning and electrostatic spraying; the sodium hyaluronate particles can greatly improve the loading rate of hydrophilic anti-inflammatory antibacterial and bone regeneration promoting medicines; a PLGA compact layer in the PLGA double-layer fiber membrane is loaded with first sodium hyaluronate particles loaded with anti-inflammatory and antibacterial drugs, and when the first sodium hyaluronate particles make contact with gingival tissue, cell adhesion and permeation can be hindered, and the anti-inflammatory and antibacterial effects can be achieved; a PLGA loose layer in the PLGA double-layer fiber membrane carries second sodium hyaluronate particles loaded with a bone regeneration promoting medicine, and the second sodium hyaluronate particles can promote osteoblast adhesion and facilitate dentale regeneration when making contact with bone tissue.

The application discloses application of a genetic lineage tracing mouse model in regulation of myocardial cell transformation into osteoblasts, and belongs to the technical field of biotechnology.The tracing mouse model is induced to have myocardial calcification through high-dose steroids or myocardial infarction operation, the tracing mouse model expresses a label protein in myocardial cells of mammals, and the label protein expression in the myocardial cells is realized through a Cre / loxP system.The genetic lineage tracing mouse model provided by the application can be used for detecting myocardial cell transformation into osteoblasts, and can also be used for developing or screening drugs capable of preventing myocardial calcification or preparing products for developing or screening the drugs capable of preventing myocardial calcification.

The invention discloses a telmisartan glucoside derivative, a microbial conversion preparation method thereof and application of the telmisartan glucoside derivative in preparation of a medicine for preventing or treating osteoporosis. The telmisartan glucoside derivative is characterized in that a telmisartan propyl side chain is connected with a glucosyl group. According to the invention, a microbial conversion technology is utilized, telmisartan is taken as a substrate, and glucosyl is introduced into a telmisartan propyl side chain in a regioselective manner, so that the telmisartan glucoside derivative with a novel structure is obtained. Experiments on the osteoblast proliferation promoting activity and the postmenopausal osteoporosis resisting effect of the telmisartan glucoside derivative by using an in-vitro osteoblast model and an ovariectomized induced osteoporosis mouse model prove that the telmisartan glucoside derivative has relatively good osteoblast proliferation promoting activity and postmenopausal osteoporosis resisting activity; the compound can be used as an active component of a medicine for treating osteoporosis, and has wide application.

This invention discloses a method for in-situ preparation of calcium chelate peptides that promote intestinal calcium absorption and osteoblast development from fish scales using high-pressure homogenization-assisted enzymatic hydrolysis. The method uses fish scales as raw material, which are pulverized and then pretreated with high-pressure homogenization to break down the dense cross-linked structure of the scales. Pepsin is used as the hydrolytic enzyme to achieve efficient enzymatic hydrolysis of collagen and in-situ chelation with natural calcium, resulting in a one-step preparation of collagen calcium chelate peptides. The product has a calcium retention rate of ≥84.16%, is rich in affinity amino acids, and shows a ≥62.97% increase in calcium transport compared to calcium chloride in a small intestinal epithelial cell absorption and transport model, while being safe and non-cytotoxic. This invention is a green and efficient process that eliminates the need for decalcification steps, achieving a collagen calcium chelate peptide yield of ≥83.4%. It realizes the high-value utilization of aquatic by-products and can be directly applied to various calcium supplements such as food and health products that enhance intestinal calcium absorption and promote bone development, solving problems such as low bioavailability of traditional calcium preparations and severe calcium loss in existing fish scale utilization processes.

The invention belongs to the technical field of food-borne functional factors, and particularly relates to a bioactive glycopeptide as well as a preparation method and application thereof. According to the application, casein glycomacropeptide is taken as a substrate, specific protease is adopted for carrying out directional enzymolysis reaction on the casein glycomacropeptide, and after primary separation and purification by hydrophilic interaction chromatography and secondary purification by cation exchange chromatography are carried out, the bioactive glycopeptide with the molecular weight of less than 1,500 Da and more than 80% of components and the random coil secondary structure content of more than 35% is obtained. A system performance test shows that in an in-vitro cell experiment, the bioactive glycopeptide prepared by the embodiment of the invention can remarkably improve the proliferation rate of preosteoblasts and lay a sufficient cell quantity foundation for bone cell generation; meanwhile, the activity of an osteogenic early differentiation key marker ALP is effectively up-regulated, and the differentiation process of preosteoblasts towards the osteoblast direction is accelerated; in addition, the expression quantity of osteogenesis-related regulatory protein calcium-sensitive receptors is remarkably increased, and the function improvement of cells to mature bone cells is further promoted.

The invention discloses a metal organic framework material targeting bone marrow fat cells as well as a preparation method and application of the metal organic framework material, and belongs to the technical field of biomedical materials and tissue engineering. The material comprises: (1) a ZIF-8 core structure used for loading nucleic acid / small molecule drugs; (2) surface modification of lipid droplet targeting peptide: the lipid droplet targeting peptide is combined with a PEDF receptor to realize precise targeting; and (3) anti-miR-188 is loaded inside and is used for inhibiting adipogenic differentiation. The method at least has the following advantages: (1) a bone marrow adipocyte specific targeting MOF material is proposed for the first time, and bone metabolism intervention is converted from a mode of targeting osteoblast or macrophage to targeting adipocyte; (2) the MOF core can quickly respond to a marrow weak acid environment to realize accurate release; (3) the AdipoPep targeting peptide can obviously prolong the residence time of the bone marrow; and (4) adipogenic differentiation is significantly inhibited, osteogenesis microenvironment reconstruction is promoted, and an excellent bone mass recovery effect is shown in an osteoporosis model.

The invention discloses an application of a branched ketonic acid dehydrogenase kinase (BCKDK) inhibitor in preparation of a medicine for preventing and treating osteoporosis. The invention finds that BCKDK can inhibit osteoblast differentiation and reduce the expression of endogenous BCKDK in osteogenic precursor cells so as to promote osteoblast differentiation; a small molecule compound inhibitor BT2 (3, 6-dichloro-2-benzothiophene carboxylic acid) of BCKDK promotes osteoblast differentiation of ovariectomized mice, inhibits osteoclast differentiation, reduces ovariectomized mouse bone loss and increases bone mass. According to the invention, the BCKDK inhibitor is proposed for the first time to be capable of promoting osteoblast differentiation, inhibiting osteoclast differentiation and increasing body bone mass by reducing BCKDK expression or inhibiting BCKDK protein biological functions, and has the effect of preventing and treating osteoporosis.

This invention discloses a biomimetic corncob scaffold with piezoelectric, antibacterial, osteogenic, and healing-promoting functions, comprising: extraction of corncob cellulose nanofibers (CNF); preparation of 3D printing ink; 3D printing of a porous scaffold; loading of antimicrobial peptides and BMP-2 onto a GelMA+PAAM composite hydrogel; and composite preparation of the scaffold and hydrogel. Under external stimulation, the scaffold generates a microcurrent / surface charge, directly disrupting bacterial cell membranes; it avoids drug release and resistance issues, employing a physical mechanism of action with a sustainable response. The microcurrent / electric field generated by the piezoelectric effect simulates the bioelectric environment of natural bone, directly stimulating osteoblast proliferation and differentiation, and promoting mineralization; it mimics the natural anisotropic porous structure of the corncob, facilitating nutrient transport and cell migration; the piezoelectric material itself provides both antibacterial and osteogenic functions, achieving synergistic and unified mechanisms; it is suitable for harsh environments, biodegradable, and has flexible activation methods.

An injectable hydrogel for inhibiting osteosarcoma growth and its component preparation method are disclosed. The hydrogel is composed of curcumin nanoparticles, nano-hydroxyapatite, and methacryloxychitosan. This injectable hydrogel for inhibiting osteosarcoma growth combines the antitumor properties of curcumin nanoparticles, the osteogenic ability and good mechanical strength of nano-hydroxyapatite, and the porous structure and injectability of methacryloxychitosan. This injectable hydrogel for inhibiting osteosarcoma growth exhibits a significant killing effect on osteosarcoma cells and simultaneously promotes the proliferation of mouse embryonic pre-osteoblasts. Through its synergistic antitumor and osteogenic dual functions, this hydrogel shows promising application prospects in postoperative treatment of osteosarcoma.

This application provides a composition containing PENK protein and its application in promoting osteoblast osteogenic differentiation. Compared with the prior art, this application has the following advantages: The pro-PENK recombinant protein and the PENK-containing composition provided in this application, after being applied to cells with osteogenic potential, enhance osteogenic performance through interaction with CBFB protein, and can significantly promote osteogenic differentiation. Compared with the existing bone morphogenetic protein 2 (BMP recombinant protein), the osteogenic effect is superior. Furthermore, experiments have verified that the osteogenic differentiation effect of pro-PENK recombinant protein on osteoblasts is dose-dependent. This application further verifies that the combined use of low doses of PENK recombinant protein and BMP recombinant protein has a more significant cellular osteogenic effect than the use of either at high doses alone.

This invention discloses a biomimetic helical chiral porous bone repair and bone augmentation scaffold, comprising: a scaffold body composed of an array of multiple lattice units, each lattice unit being a biomimetic trabecular bone cell; the lattice units follow the natural helical arrangement of human bone trabeculae, forming a continuous and interconnected helical channel network within the scaffold body; the lattice units are distributed in a helical gradient along the radial direction of the scaffold body, and the outer wall of the scaffold body has a protruding structure integrally formed with the lattice units. This invention, through the deep coupling of the biomimetic helical chiral structure and cellular fluid dynamics, overcomes the limitations of traditional scaffolds with "static channels," forming a composite flow field of "high-speed guidance - low-speed vortex," effectively solving the problem of "nutrient islands," and significantly improving the survival rate and quality of osteoblasts in the central region.

The invention discloses hydrogel for osteoporotic bone defect repair and a preparation method of the hydrogel. The hydrogel comprises basic hydrogel and mineralized photosynthetic microorganisms, the mineralized photosynthetic microorganism comprises a photosynthetic microorganism, a mineralized shell layer arranged on the surface of the photosynthetic microorganism, and rare earth up-conversion nano-particles loaded in cells of the photosynthetic microorganism, and the rare earth up-conversion nano-particles can convert near-infrared light into visible light. According to the present invention, the oxygen production type mineralized microalgae composite hydrogel with characteristics of deep tissue activation, controllable oxygen production and simultaneous realization of four-effect synergy of acid neutralization, continuous oxygen supply, angiogenesis promotion and osteogenesis mineralization can be provided, such that osteoblast activity can be promoted, osteoclast activity can be inhibited, in-vivo osteogenesis can be promoted, and the technical support can be provided for osteoporotic bone defect repair.

The invention relates to the technical field of biology, in particular to hyriopsis cumingii exosome-like nano vesicles and application thereof in preparation of osteogenesis products. According to the preparation method, hyriopsis cumingii is taken as a raw material, and the hyriopsis cumingii exosome-like nano-vesicles are obtained through separation and purification by adopting a differential centrifugation and ultracentrifugation combined method; the nano-vesicle has a complete membrane structure and typical exosome-like morphological characteristics, in-vitro experiments prove that the nano-vesicle can significantly promote proliferation of mouse cranial preosteoblasts (MC3T3-E1) and shows osteogenic activity, and a new way is provided for development of aquatic product-derived osteogenic functional factors.

This invention discloses a collagen calcium chelating peptide and its application in the preparation of calcium supplements and osteoporosis prevention products, belonging to the field of food biotechnology. Osteogenic activity evaluation confirmed that the collagen calcium chelating peptide significantly promoted MC3T3-E1 cell proliferation, increased alkaline phosphatase activity, and enhanced mineralized nodule formation within a concentration range of 1-100 μg / mL, exhibiting a clear concentration-dependent effect. Intestinal calcium absorption promotion experiments showed a significant increase in calcium transport compared to the calcium chloride control group, and fluorescence imaging confirmed its effective enhancement of calcium ion uptake in small intestinal epithelial cells. This invention clarifies for the first time the structural characteristics and dual biological activities of a fish scale-derived calcium chelating peptide, revealing its mechanism of action in regulating osteoblasts through the EGFR signaling pathway. This provides a new pathway for the high-value utilization of aquatic by-products and offers a scientific basis and high-quality candidate raw materials for the development of novel functional calcium supplements.

This invention provides a polylactic acid / zinc-doped hydroxyapatite composite material for bone fixation devices, its preparation method, and its applications. The invention utilizes a melt blending method to prepare composite materials with different formulation ratios under optimal process conditions. When the zinc-doped hydroxyapatite content is 10%, the composite material exhibits the best thermal stability and mechanical properties (tensile strength of 63.2 MPa). The composite material shows the lowest rate of flexural performance degradation after 20 days of degradation. In vitro degradation experiments demonstrate that the mechanical properties of the composite material can be altered by changing the zinc-doped hydroxyapatite content, and the degradation rate can be modified by changing the zinc-doped hydroxyapatite content to meet the requirements of the implantation site. Cytotoxicity experiments show that an appropriate zinc ion concentration can promote the cell activity of mouse osteoblasts.

The present application belongs to the technical field of hydrogel materials, and particularly relates to a composite hydrogel for regulating energy metabolism and a preparation method and application thereof. The preparation raw material of the composite hydrogel for regulating energy metabolism comprises creatine modified double bond hydroxybutyl chitosan, hydroxyapatite, curcumin, an initiator and a solvent. CHBC provides energy metabolism support at the cell level, is conducive to the proliferation and differentiation of osteogenesis-related cells; HA provides an inorganic environment similar to bone mineralization, and plays a bone conduction and bone induction role; Cur regulates inflammatory response and improves oxidative stress state, and optimizes the repair microenvironment. Through synergy in different aspects such as energy metabolism support, structure mineralization and microenvironment regulation, the regulation of the energy metabolism state of cells in the bone repair area is realized, which is conducive to meeting the high energy requirement of osteoblasts in the repair process, and can promote new bone formation and improve the inflammatory and oxidative stress microenvironment of the injury area, and provides more favorable repair conditions for bone tissue regeneration.

The invention discloses establishment and application of an epinephelus coioides frontal bone tissue osteoblast line, and relates to the technical field of biology. The cell line is an epinephelus coioides frontal bone tissue osteoblast line ECFBOB, the preservation number is CCTCC NO: C2025131, the cell line is derived from the frontal bone tissue of juvenile epinephelus coioides, and the cell line is established after tissue separation, primary culture by a dry pasting method, subculture and cryopreservation resuscitation. The obtained cell line can be used as an in-vitro research model, provides a key technical tool for saddletail grouper improved variety breeding and healthy culture in saddletail grouper skeletal development mechanism analysis, osteogenesis-related gene function verification, craniofacial bone malformation cause research and species traceability verification, fills the blank of saddletail grouper bone tissue cell lines, and has a wide application prospect. The method has important scientific research value and industrial application prospect.

The invention relates to the technical field of biology, in particular to novel application of sanguinarine (SAN), a method for inducing human amniotic mesenchymal stem cells (hAMSCs) to be differentiated into osteoblasts through COX2 protein and an osteogenic induction culture medium for the inducing method, and the method can be applied to the fields of bone disease treatment drug research and development and clinical transformation. It is proved for the first time that SAN mediates hAMSCs osteogenic differentiation through COX2 protein, SAN can significantly improve expression of COX2 gene (PTGS2) in hAMSCs, the osteogenic effect of SAN can be reversed by adding a COX2 inhibitor (such as celecoxib), and direct experimental evidence is provided for'COX2 serving as a small molecule drug to promote osteogenic differentiation '.

The invention discloses lactobacillus plantarum and application thereof in promoting bone health and regulating intestinal flora. The name of the lactobacillus plantarum is lactobacillus plantarum GXS77, the preservation number of the lactobacillus plantarum is CGMCC No.35899, and the lactobacillus plantarum is preserved in China General Microbiological Culture Collection Center (CGMCC) in No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing in 2025. The yield of acetic acid and the yield of butyric acid of the lactobacillus plantarum GXS77 obtained through screening are increased by 18% and 16% respectively compared with the yield of acetic acid and the yield of butyric acid of common lactobacillus plantarum, the peak calcium absorption capacity of Caco-2 cells can be increased by 12.3%, osteoblasts are differentiated, and then bone health is promoted; meanwhile, the lactobacillus plantarum GXS77 has good acid resistance, cholate resistance and a good cell adhesion effect. Therefore, the strain can be used for developing fermented food, health-care products and medicines with the effects of promoting bone health, regulating intestinal flora and the like.

The invention discloses a peptide YIYT with osteoporosis relieving and antioxidant activity as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the lactoferrin hydrophobic peptide YIYT is tyrosine-isoleucine-tyrosine-threonine (Tyr-Ile-Tyr-Thr, YIYT), and the lactoferrin hydrophobic peptide YIYT has a structural formula shown in the description. Experiments prove that YIYT can significantly promote proliferation, differentiation and mineralization of osteoblasts, accelerate the cell cycle process, inhibit osteoclast activity, reduce bone resorption level and recover bone metabolic balance. In an animal model, the YIYT can effectively improve the bone density of ovariectomized mice and improve a bone-related microstructure, and has double effects of promoting bone formation and inhibiting bone resorption. The lactoferrin hydrophobic peptide YIYT provided by the invention is safe in source and clear in action mechanism, and provides a new candidate substance and an application direction for developing a novel anti-osteoporosis medicine.

Aortic valve calcification is a condition in which calcium deposits form on the aortic valve in the heart. These deposits can cause narrowing at the opening of the aortic valve. This narrowing can become severe enough to reduce blood flow through the aortic valve—a condition called aortic valve stenosis. The inventors have shown that retinoic acid decreases calcification and osteoblast-like phenotype in valvular interstitial cells (VICs). More particularly, RARα activation reduces calcification and osteoblast-like phenotype in VIC. On the contrary, ALDH1A1 inhibition increases calcification and osteoblast-like phenotype in VIC. Thus the results prompt to consider that use or retinoic acid receptor (RAR) agonists would be suitable for the reversing, preventing or delaying calcification of the aortic valve.

The invention discloses establishment and application of a humpback perch frontal bone tissue osteoblast line, and relates to the technical field of biology. The cell line is a humpback perch frontal bone tissue osteoblast line CAFBOB, the preservation number is CCTCC NO: C2025129, and the cell line is derived from a humpback perch juvenile fish frontal bone tissue and is established after tissue separation, primary culture by a dry pasting method, subculture and cryopreservation resuscitation. The biological characteristics are stable, 1-40 generations are mainly long fusiform, the chromosome karyotype is 2n = 48, strong alkaline phosphatase activity is achieved, the transfection efficiency is about 85%, and the activity is good after cryopreservation and resuscitation. The establishment method is high in repeatability, the obtained cell line can be used as an in-vitro research model and applied to morphological specialization mechanism analysis, osteogenesis-related gene function verification, classification dispute solution and seeding process research of the humpback perch, the blank of the bone tissue cell line of the humpback perch is filled, and the bone tissue cell line has important scientific research value and application prospect.

Embodiments of the disclosure include methods and compositions related to modulation of bone using particular fibroblasts. The modulation includes reducing osteoclast activity and / or activation and / or stimulating osteoblast activity. In particular cases, bone is modulated in an individual with a bone condition, such as osteoporosis. Particular fibroblasts may be delivered to reduce inflammatory cytokine production including RANK ligand.

This invention relates to the field of cell culture technology, specifically to a serum-containing osteogenic induction differentiation medium, a culture method, and its application. The differentiation medium consists of a basal medium and supplementary components. The basal medium is MEMα, and the supplementary components consist of component A and component B. Component A includes fetal bovine serum, L-ascorbic acid-2-phosphate magnesium salt, β-glycerophosphate sodium, dexamethasone, resveratrol, estradiol, strontium chloride, vitamin K2, and sodium butyrate; component B is 1α,25-dihydroxyvitamin D3. This invention exhibits high differentiation efficiency. Mesenchymal stem cells induced using the differentiation medium of this invention show obvious calcium nodules within 7 days, and osteoblast differentiation is achieved within 10-12 days.

[Problem] The present invention provides an inhibitor or the like, which contains 3, 5-dihydroxy-4-methoxybenzyl alcohol (3, 5-dihydroxy-4-methyloxbenzene alcohol, DHMBA) as an active ingredient, and which is capable of providing a new strategy for preventing and treating bone formation disorders and mineralization disorders of osteoblasts, which are associated with an inflammatory state in the bone marrow microenvironment. [Solution] The present invention is characterized by having 3, 5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) as an active ingredient and having an effect of promoting mineralization of osteoblast MC3T3-E1 cells.