3D (three-dimensional) uniform porous scaffold material and preparing method thereof

A porous scaffold, uniform technology, applied in the field of biomedical materials, can solve the problems of affecting cell adhesion, high pore size, affecting cell migration and proliferation, etc.

Active Publication Date: 2014-08-20
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the pore size of the scaffold material is less than 100 μm, the scaffold is too dense and the porosity is too small, and the small pore size will affect the migration and proliferation of cells in the material. In addition, as an implant in the body, the material with small pore size will form a hypoxic zone and The transmission of water and nutrients is limited, which is not conducive to the formation of vascularization; however, when the pore size and porosity of the scaffold material are too large, it will affect the adhesion of cells on the surface of the material and affect the communication between cells. It is not conducive to the construction of extracellular matrix
[0004] Most of the porosity of the prepared three-dimensional porous materials cannot meet the above technical requirements, and the porosity is relatively small, which limits the migration of cells and the formation of new tissues to a certain extent; or the distribution of micropores Inhomogeneous, in the process of mutual communication between the material and the body in the early stage of implantation, it is not conducive to the distribution of osteoblasts on the surface of the material, and ultimately affects the quality of new tissue; or has a relatively high pore size, which is not conducive to a certain extent The adhesion and proliferation of cells on the surface of the material affect the establishment of the extracellular matrix

Method used

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  • 3D (three-dimensional) uniform porous scaffold material and preparing method thereof
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  • 3D (three-dimensional) uniform porous scaffold material and preparing method thereof

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preparation example Construction

[0032] A method for preparing a 3D uniform porous scaffold material, characterized in that:

[0033] Achieved by the following steps:

[0034] Step 1: Dissolving collagen in sodium carbonate solution so that the final mass fraction of collagen reaches 10-30%;

[0035] Step 2: Add nano-hydroxyapatite to the mixture in step 1, the mass ratio of collagen to nano-hydroxyapatite is 1: (3-5), shake on the vortex mixer until the mixture is uniform, and the viscosity is uniform like emulsion;

[0036] Step 3: distributing the mixture obtained in step 2 into moulds;

[0037] Step 4: The mold is frozen at -80°C for 3-6 hours, and then vacuum-dried for 48 hours;

[0038] Step 5: carry out the cross-linking reaction of the primary freeze-dried product in step 4 in an excess of cross-linking agent solution, 100ml of cross-linking agent solution corresponds to 12-15g of the primary freeze-dried bone scaffold material, the pH of the cross-linking system is 8-11, The reaction temperature ...

Embodiment 1

[0050] Step 1: dissolving collagen in sodium carbonate solution so that the final mass fraction of collagen reaches 10%;

[0051] Step 2: Add nano-hydroxyapatite to the mixture in step 1. The mass ratio of collagen to nano-hydroxyapatite is 1:3. Shake on a vortex mixer until evenly mixed to obtain a viscous and uniform emulsion ;

[0052] Step 3: distributing the mixture obtained in step 2 into moulds;

[0053] Step 4: The mold is frozen at -80°C for 3 hours, and then vacuum-dried for 48 hours;

[0054] Step 5: Carry out the cross-linking reaction of the primary freeze-dried product in step 4 in an excess of cross-linking agent solution, 100ml of cross-linking agent solution corresponds to 12g of the primary freeze-dried bone scaffold material, the pH of the cross-linking system is 8, and the reaction temperature is 37 °C, the reaction time is 24h;

[0055] Step 6: Perform a secondary cross-linking reaction on the sample cross-linked in step 5 in an excess of cross-linking ...

Embodiment 2

[0066] Step 1: dissolving collagen in sodium carbonate solution so that the final mass fraction of collagen reaches 20%;

[0067] Step 2: Add nano-hydroxyapatite to the mixture in step 1. The mass ratio of collagen to nano-hydroxyapatite is 1:4. Shake on a vortex mixer until evenly mixed to obtain a viscous and uniform emulsion ;

[0068] Step 3: distributing the mixture obtained in step 2 into moulds;

[0069] Step 4: The mold is frozen at -80°C for 4.5 hours, and then vacuum-dried for 48 hours;

[0070] Step 5: Carry out the cross-linking reaction of the primary freeze-dried product in step 4 in an excess of cross-linking agent solution, 100ml of cross-linking agent solution corresponds to 13g of the primary freeze-dried bone scaffold material, the pH of the cross-linking system is 9, and the reaction temperature is 48 ℃, the reaction time is 30h;

[0071] Step 6: Perform a secondary cross-linking reaction on the sample cross-linked in step 5 in an excess of cross-linking...

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Abstract

The invention relates to a 3D (three-dimensional) uniform porous scaffold material and a preparing method of the 3D uniform porous scaffold material. The existing artificial bone scaffold material has the defects that the porosity is low, and the cell migration and the new tissue formation are limited; the micropore diameter distribution is nonuniform, and the condition is unfavorable for the distribution of osteoblast cells and the like on the surface of the material; and the pore diameter is greater, and the condition is unfavorable for the cell adhesion and cell proliferation. According to the preparing method, collagen is dissolved in a sodium carbonate solution; nanometer hydroxyapatite is added; the mixture is subpackaged into a mold; the mold is subjected to freezing and vacuum drying; a cross-linking reaction is carried out on the frozen and dried sample in an alkaline cross-linking environment; a cross-linking reaction is carried out on the cross-linked sample in an acid environment; the obtained sample is washed; and the washed sample is subjected to vacuum freeze drying, sterilization and packaging. The 3D uniform porous scaffold material and the preparing method have the advantages that the collagen and the nano-hydroxyapatite are used as raw materials, and carbon dioxide produced by reaction between sodium carbonate and alkali and ice crystal sublimation carried out in the vacuum freeze drying process are utilized, so that a micropore structure is obtained with uniform pore diameter distribution and with a pore diameter and porosity suitable for cell adhesion, proliferation and migration, and a uniform porous scaffold obtained through preparation has certain pressure resistance intensity and has a good microstructure and good biocompatibility.

Description

technical field [0001] The invention relates to a biomedical material, in particular to a 3D uniform porous scaffold material and a preparation method thereof. Background technique [0002] The proposal and development of regenerative medicine and tissue engineering have brought new hope for the repair and treatment of bone defects clinically. With the formation of new tissue in a real sense, the tissue engineering scaffold needs to be used as a temporary support frame system at the repair site, capable of maintaining cell adhesion, migration and differentiation, and maintaining nutrients, water and metabolic excretions. The input and output, which requires the implant material scaffold to have a three-dimensional interpenetrating micro-architecture, and at the same time have a suitable pore size. Therefore, the adhesion, proliferation and climbing of cells on the surface and inside of the material are the key to the repair of new bone at the implant site. [0003] Studie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08J9/08C08J3/24A61L27/56A61L27/42A61L27/24A61L27/12
Inventor 范代娣马晓轩惠俊峰米钰范慧
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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