Preparation method of multifunctional layered joint cartilage support

A multi-functional articular cartilage technology, applied in medical science, prosthesis, additive processing, etc., can solve the problems of bionic distribution of cell density in artificial cartilage, achieve regulation, realize growth and development, and reduce rejection Effect

Inactive Publication Date: 2018-08-03
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the method of casting after mixing the raw materials, the composition distribution of each layer i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] This embodiment uses the preparation of rat knee joint artificial cartilage to describe a method for preparing a multifunctional layered articular cartilage scaffold, which specifically includes the following steps:

[0060] Step 1. Obtain a three-dimensional model of the cartilage tissue to be printed, and use computer processing to separate the three-dimensional model of the cartilage layer, the calcified layer, and the subchondral bone layer. The three-dimensional models of the cartilage layer, calcification layer, and subchondral bone layer were sliced ​​and sliced ​​separately. The layer thickness was 0.1mm. The cartilage layer was divided into N 1 The cartilage layer is divided into N 2 The subchondral bone layer is divided into N 3 Layers to obtain layered cross-sectional data.

[0061] Step 2: Culture chondrocytes in high-sugar DMEM medium to obtain a cell density of 5×10 6 cells / ml chondrocyte suspension; hypoxia (5% O 2 ) Cultivating bone marrow mesenchymal stem cel...

Embodiment 2

[0081] This embodiment uses the preparation of rabbit knee joint artificial cartilage to describe a method for preparing a multifunctional layered articular cartilage scaffold, which specifically includes the following steps:

[0082] Step 1. Obtain a three-dimensional model of the cartilage tissue to be printed, and use computer processing to separate the three-dimensional model of the cartilage layer, the calcified layer, and the subchondral bone layer. The three-dimensional models of the cartilage layer, calcification layer, and subchondral bone layer were sliced ​​and sliced ​​separately. The layer thickness was 0.1mm. The cartilage layer was divided into N 1 The cartilage layer is divided into N 2 The subchondral bone layer is divided into N 3 Layers to obtain layered cross-sectional data.

[0083] Step 2: Culture chondrocytes in high-sugar DMEM medium to obtain a cell density of 7×10 6 cells / ml chondrocyte suspension; hypoxia (5% O 2 ) Cultivating bone marrow mesenchymal stem ...

Embodiment 3

[0103] This embodiment uses the preparation of rabbit knee joint artificial cartilage to describe a method for preparing a multifunctional layered articular cartilage scaffold, which specifically includes the following steps:

[0104] Step 1. Obtain a three-dimensional model of the cartilage tissue to be printed, and use computer processing to separate the three-dimensional model of the cartilage layer, the calcified layer, and the subchondral bone layer. The three-dimensional models of the cartilage layer, calcification layer, and subchondral bone layer were sliced ​​and sliced ​​separately. The layer thickness was 0.1mm. The cartilage layer was divided into N 1 The cartilage layer is divided into N 2 The subchondral bone layer is divided into N 3 Layers to obtain layered cross-sectional data.

[0105] Step 2: Culture chondrocytes in high-sugar DMEM medium to obtain a cell density of 5×10 6 cells / ml chondrocyte suspension; hypoxia (5% O 2 ) Cultivating bone marrow mesenchymal stem ...

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Abstract

The invention provides a preparation method of a multifunctional layered joint cartilage support. The multifunctional layered joint cartilage support capable of simulating the biological property andthe mechanical property of natural cartilage is prepared by taking hydrogel as a support material, taking mesenchymal stem cells, cartilage cells and osteoblasts as inducing and developing objects andby adopting a 3D printing technology. The multifunctional layered joint cartilage support can accurately simulate the internal tissue form and the contour of each layer of the natural cartilage and can realize that different layers have different mechanical and biological characteristics. The prepared artificial cartilage support cells are derived from patients, the support serves as a degradablehydrogel material and the rejection reaction after implantation is greatly reduced. Various nutritional substances and growth factors are mixed in the hydrogel support, so that the sustained releaseeffect can be achieved and regulation and control of the growth and development of the artificial cartilage can be realized.

Description

Technical field [0001] The invention belongs to the field of cartilage tissue repair, and specifically relates to a method for preparing a multifunctional layered articular cartilage scaffold. Background technique [0002] Articular cartilage has almost no ability to repair itself. Cartilage damage is often irreversible, often causing osteoarthritis and even life-threatening. Defects of osteochondral composite tissue in joints have become one of the main causes of limb disabilities. [0003] At present, the method of implanting substitutes is mainly used to treat articular cartilage lesions. The substitutes mainly include autologous or foreign tissues, artificial cartilage and so on. Among them, autologous or allogeneic tissues have problems such as limited source and difficult shape matching. Artificial cartilage has a wide development space due to its wide source of raw materials and good biological adaptability. Artificial cartilage has preparation methods such as biomimetic m...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/38A61L27/22A61L27/20A61L27/12A61L27/50A61L27/52A61L27/54B33Y10/00B33Y50/02B33Y70/00B33Y80/00
CPCA61L27/12A61L27/20A61L27/222A61L27/3817A61L27/3821A61L27/3834A61L27/3852A61L27/3895A61L27/50A61L27/52A61L27/54A61L2300/414A61L2430/06B33Y10/00B33Y50/02B33Y70/00B33Y80/00C08L5/04
Inventor 汪焰恩国玉鸿魏庆华蔡显轩宋瑶王国伟柴卫红杨明明张坤
Owner NORTHWESTERN POLYTECHNICAL UNIV
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