40results about How to "Biological activity has" patented technology

The invention relates to a preparation method for a microarc oxidation/hydrothermal treatment composite film on the surface of a magnesium alloy. The problem that the existing magnesium alloy is poor in corrosion resistance and bioactivity is solved. A microarc oxidation film is prepared on the magnesium alloy by using a microarc oxidation method, and then a bioceramic film is obtained by using hydrothermal treatment. Hydrothermal reaction liquid is composed of sodium hydroxide, calcium acetate and sodium hexametapolyphosphate. In the preparation method, the microarc oxidation is combined with the hydrothermal treatment; calcium and phosphorus are introduced to the surface of the microarc oxidation film by using the hydrothermal treatment; in addition, holes in the ceramic film are blocked by using hydrothermal treatment; therefore, corrosive media are effectively prevented from entering; the corrosion resistance of the composite film is effectively improved; and the corrosion resistance of the composite film to simulated body fluid is greatly improved by approximately 2 orders of magnitude.

Disclosed are an artificial tooth root with a micro-nano hierarchical topologic surface structure and a preparation method of the artificial tooth root. The artificial tooth root is prepared by compounding a porous titanium dioxide surface layer with a zinc oxide nanorod or nanocone sedimentarily growing on the surface pore wall of the porous titanium dioxide surface layer, wherein the porous titanium dioxide surface layer is prepared with pure titanium or titanium alloy matrix by a microarc oxidation method, namely, the porous titanium dioxide bioactivity surface layer with an adjustable pore size is obtained by adjusting microarc oxidation parameters on the titanium matrix surface of the artificial tooth root, and the zinc oxide nanorod/nanocone is sedimentarily prepared on the titanium dioxide bioactivity surface layer by further adjusting electro-deposition parameters, so that the artificial tooth root with the micro-nano hierarchical topologic surface structure is formed. By the aid of the titanium dioxide micron porous structure with a rich surface and the zinc oxide nano-topologic structure sedimentarily growing on the pore wall of the titanium dioxide micron porous structure, the artificial tooth root can enhance biological effects of adhesion, growth, multiplication and the like of surface osteoblasts, slowly release zinc, and greatly improve antibacterial activity, biological activity, biocompatibility and cell regulation capacity of the surface in the early stage of implantation.

The invention discloses a method for preparing a biological activity micro-arc oxidation ceramic coating with a macroscopic/microcosmic double-stage pore structure on the surface of medical titanium through a two-step method, relates to a method for preparing micro-arc oxidation ceramic coating, and solves the problems that a conventional method for preparing double-stage pore micro-arc oxidation ceramic coating is more complicated in preparation process, and the prepared coating has no biological activity. The method comprises the following steps: first, carrying out surface pretreatment on a titanium slab sample, then, placing the pretreated titanium slab sample in sodium nitrate containing micro-arc oxidation electrolyte to be primarily oxidized, next, replacing the oxidized sample in silicon-calcium-phosphate-sodium electrolyte to be secondarily oxidized, washing for 3-5 times, and drying to obtain the biological activity micro-arc oxidation ceramic coating generated on the surface of the titanium slab. The invention can be applicable to the preparation of the biological activity micro-arc oxidation ceramic coating with the macroscopic/microcosmic double-stage pore structure on the surface of medical titanium.

The invention provides an artificial joint with a micro-nano graded topological surface structure and a preparation method of the artificial joint. The artificial joint is formed by compounding a titanium alloy substrate, a micro-porous titanium dioxide surface layer prepared by a micro-arc oxidization method, and a zinc oxide nano rod or a nano taper deposited and grown on the hole wall of the surface, namely the micro-porous titanium dioxide biological activity surface layer is obtained on the surface of an artificial joint titanium substrate through the micro-arc oxidization method; and furthermore, electric depositing parameters are adjusted and a zinc oxide nano rod/taper is deposited and prepared on the titanium dioxide biological activity surface layer, so as to form the artificial joint with the micro-nano graded topological surface structure. The artificial joint can utilize abundant titanium dioxide micron hole structures on the surface and a zinc oxide nano topological structure deposited and grown in the hole wall to enhance the biological effects of adhesion, growth, multiplication and the like of surface osteoblast, and realize the slow release of the element zinc; and therefore, the antibacterial capability, the biological activity, the biocompatibility and the cell regulating and controlling capability of the surface at an implantation initial stage are greatly improved.

The invention relates to a bioactive porous poly-ether-ether-ketone material and a preparation method and application thereof. The active porous poly-ether-ether-ketone material has a pore diameter of450-800 [mu] m and a porosity of 30-95%, and hydroxyapatite microspheres are contained on the inner surfaces of pores. The preparation method comprises the following steps: uniformly adhering the hydroxyapatite microspheres to the surfaces of NaCl particles, performing sintering to obtain composite pore-forming particles, uniformly mixing the composite pore-forming particles with poly-ether-ether-ketone, performing compacting, performing sintering, and finally removing NaCl to obtain the porous poly-ether-ether-ketone material. Osteogenesis promoting medicines and antibacterial medicines canbe loaded into the material according to needs, and the hydroxyapatite microspheres on the inner surface are added, so that the bioinertness of the poly-ether-ether-ketone can be effectively improved,the poly-ether-ether-ketone has bioactivity, the medicines and growth factors can be loaded, a good slow release effect is achieved, and good osteogenesis promoting and antibacterial properties are achieved. The bioactive porous poly-ether-ether-ketone material disclosed by the invention has the advantages of simple process, low cost, controllable structure, convenient drug loading and the like.

The invention provides a multi-bioactivity modified lignin self-assembled drug-loaded nano-micelle. The multi-bioactivity modified lignin self-assembled drug-loaded nano-micelle is prepared by acylating lignin modified by anhydride and then modifying the acylated lignin by a bioactive compound. The bioactive compound has a variety of biological activities, lignin is subjected to acylation, esterification and other reactions to prepare the lignin grafted bioactive compound, then the lignin grafted bioactive compound is prepared into the nano-micelle, and the nano-micelle is adopted as a good drug carrier, and further has a variety of biological activities of lignin and the bioactive compound; the bioactive compound and the lignin are connected together through chemical bonds, and the nano-micelle is self-assembled by utilizing the amphipathy of the lignin, so that the utilization rate of the bioactive compound can be greatly improved; a hydrophobic unit of the bioactive compound is introduced into the lignin, so that the drug loading capacity of the nano-micelle to hydrophobic drugs can be greatly improved. The invention further provides a preparation method of the multi-bioactivitymodified lignin self-assembled drug-loaded nano-micelle, the process is simple, and the drug-loading capacity of the micelle is good.

The invention relates to an extract of fenugreek seeds, its preparing process and use thereof, wherein the extract contains 60-80 wt% of polysaccharides. Its preparing process comprises, making water boiled liquid of fenugreek seeds through grilling, charging ethanol for deposition and separation, discarding supernatant fluid through centrifugation, removing ethanol from the precipitate with vacuum distillation method, finally drying to obtain the extract of fenugreek seeds, which can be used for preparing the medicament for treating diabetes and diabetic nephropathy.

The invention provides a chewing gum with a dental caries controlling function, and a preparation method thereof. The invention relates to the field of chewing gum. The chewing gum is prepared from the raw materials of, by weight, 15-60% of gum base, 12-45% of xylitol, 1-15% of Arabic gum, 5-10% of calcium lactate, 1-10% of bio-active glass micro-beads, 0.05-3% of edible flavor, 0.05-2% of lecithin, and 0.05-1% of citric acid. According to the invention, the bio-active glass micro-beads are added into the chewing gum composition as an abrasive. When the added abrasive covers a dental caries part under a human oral physiological environment, chemical bonding occurs, and the teeth are not damaged.

The invention relates to the protein field, in particular to bioactive polypeptide DKLAQ as well as a preparation method and an application thereof. An amino acid sequence of the bioactive polypeptideDKLAQ is Asp-Lys-Leu-Ala-Gln. In vitro anti-oxidation experiments and in vivo anti-aging experiments prove that the polypeptide DKLAQ has the better anti-oxidation bioactivity and anti-aging activity, on one hand, the bioactive polypeptide DKLAQ has better anti-oxidation activity and can scavenge free radicals in organisms and improve the quality of life; on the other hand, the bioactive polypeptide DKLAQ can improve the vitality of in vivo anti-peroxidase systems and enhance functions of organisms for resisting exogenous stimulation to reduce the probability of organism aging, senescence anddisease and has very important significance in development of food, healthcare products and drugs with anti-oxidation and anti-aging functions.

The invention relates to the field of proteins, particularly to a biological active polypeptide DDVTEVM, a preparation method and applications thereof, wherein the amino acid sequence of the biological active polypeptide DDVTEVM is Asp-Asp-Val-Thr-Glu-Val-Met. According to the present invention, the results of in vitro anti-oxidation experiments and in vivo anti-aging experiments verify that the polypeptide DDVTEVM has good anti-oxidation biological activity and good anti-aging activity; the biological active polypeptide DDVTEVM has good anti-oxidation activity and can remove free radicals soas to improve the quality of life; and the biological active polypeptide DDVTEVM can improve the in vivo anti-peroxidase activity and enhance the resistance of the body to exogenous stimuli so as to reduce the probability of aging and illness of the body, such that the biological active polypeptide DDVTEVM has important significance in the development of food with anti-oxidation and anti-aging functions.

The invention belongs to the technical field of medicines, and relates to Biliatresone separated and purified from plants, as well as a preparation method, medicinal composition and application thereof. The Biliatresone further comprises cis-trans-isomer, enantiomer, diastereoisomer, raceme, solvate, hydrate or pharmaceutically acceptable salt and ester. Bioactivity experimental tests indicate that the compound can be used for remarkably inhibiting zebrafish larvae from gallbladder dysplasia, and prove that the compound Biliatresone has bioactivity, can be further used as biliary epithelia-philic medicines for studying biliary atresia inducing animal models.

The invention relates to the field of protein, in particular to bioactive polypeptide LPYPYYA as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide LPYPYYA is Leu-Pro-Tyr-Pro-Tyr-Tyr-Ala. In vitro immune function regulation experiments and in vivo anti-aging experiments prove that the polypeptide LPYPYYA has better immune regulation functionand anti-aging activity; on one hand, the bioactive polypeptide LPYPYYA can enhance the in vitro multiplication capacity of lymphocytes and macrophages, improve the capability of the body for resisting external pathogen infection and reduce the disease incidence of the body; on the other hand, the activity of an in vivo antiperoxidase system can be improved; the function of the body for resistingthe exogenous irritation is enhanced, so that the body aging, senility and sickness probability is reduced; very important significance is realized on developing food, health products and medicines with the immune regulation function and the anti-aging function.

The invention relates to the field of protein, in particular to bioactive polypeptide APMISAASVH, as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide APMISAASVH is Ala-Pro-Met-Ile-Ser-Ala-Ala-Ser-Val-His. In vitro antioxidant experiments and in vivo anti-aging experiments prove that the polypeptide APMISAASVH has good antioxidant biologicalactivity and anti-aging activity; on the one hand, the bioactive polypeptide APMISAASVH has good antioxidant activity and can eliminate free radicals in bodies and improve the life quality, and on the other hand, the bioactive polypeptide APMISAASVH can improve the vitality of in vivo peroxidase resistance system, and enhance the function of resisting exogenous stimulation of bodies, thereby reducing the probability of body aging, aging and sickness, and having great importance on the development of food, health products and medicines with an antioxidant function and an anti-aging function.

The invention relates to bioactive polypeptide IQIVQNEGR as well as a preparation method and application thereof. An amino acid sequence of the polypeptide is Ile-Gln-Ile-Val-Gln-Asn-Glu-Gly-Arg, andin-vitro and in-vivo experiments prove that the polypeptide IQIVQNEGR has relatively good antioxidant and anti-aging biological activity, immunoregulation function and the like. The polypeptide has good application prospects in the aspects of resisting and blocking the damage of oxygen free radicals to the body, delaying the aging of the body, reducing oxidative stress injury and the like. The polypeptide has very important significance in developing foods, health care products, cosmetics and medicines with antioxidant and anti-aging functions.

The invention discloses a guided tissue regeneration membrane containing a TPE-NIM fluorescent probe and a preparation method of the guided tissue regeneration membrane. The preparation method comprises the following steps: (1) synthesizing TPE-Br; (2) synthesizing TPE-B (OH) 2; (3) synthesis of 4-bromine-N-(dimethylaminopropyl)-1, 8-naphthalimide is carried out; and (4) synthesizing the TPE-NIM. The GTR membrane which contains the slow-release antibacterial component, does not generate drug resistance and has bacterial traceability is used in a guided tissue regeneration technology (GTR), a membrane material is used as an isolation barrier, and the GTR membrane is placed at a periodontal tissue defect position to block gingival epithelium, prevent the gingival epithelium from growing along a root surface in a healing process and block fibrous connective tissue from being in contact with the surface of a tooth root, so that the healing effect of the gingival epithelium is improved. Therefore, periodontal ligament cells with the ability of forming new adhesion are guided to occupy the root surface preferentially, so that new adhesion is formed, and a growth space is provided for bone tissue regeneration.

The invention relates to an establishment method and application of a tumor cell three-dimensional model, and the establishment method comprises the following steps: (1) preparing platelet-rich fibrinpowder, and carrying out aseptic preservation; (2) preparing platelet-rich fibrin powder-bioactive material composite porous microspheres, and carrying out aseptic preservation; and (3) co-culturingthe tumor single cells and the composite porous microspheres to obtain a tumor cell three-dimensional model, wherein the degradation starting time of the bioactive material is shorter than the co-culture time. The application is as follows: the tumor cell three-dimensional model is used as an anti-tumor drug screening model or used for establishing a mouse tumor pathological model. The establishment method disclosed by the invention is simple, has excellent component simulation on tumor microenvironments such as tumor extracellular matrix and the like, can be used for rapidly evaluating the drug effect of the anti-tumor drug in a high-throughput manner, greatly improves the preclinical prediction efficiency and is easy to popularize.

The invention discloses an anthraquinonoimidazole nucleoside analog and a synthetic method and application thereof, and belongs to the field of chemical and medical technologies. The key points of thetechnical scheme are as follows: the structural formula of an anthraquinonoimidazole nucleoside analog is as shown in the specification. The invention also discloses a synthetic method of the anthraquinonoimidazole nucleoside analog and application of the anthraquinonoimidazole nucleoside analog in the preparation of an anti-cancer drug. By linking various five-membered sugars and six-membered sugars to an anthraquinone compound through imidazoles, a series of anthracene nucleus antibiotics and nucleoside analogs with novel structures and biological activity are obtained.

The invention discloses a preparation method for a 5-sulfuryl-fluoride-substitued isoxazoles compound. The method comprises the following steps: using substituted 2-aryl chlorinated oxime containing different functional groups as a raw material, mixing with 1-brominated vinyl sulfonyl fluorine in a solvent, adding a basic catalyst, reacting for 1-12 hours in 20-40 DEG C, separating and purifying,to obtain the 5-sulfuryl-fluoride-substitued isoxazoles compound. The obtained product by the preparation method is the isoxazoles compound substituted by 5-sulfuryl-fluoride. The preparation method comprises the following steps: through a simple 1,3-dipolar-cycloaddition reaction, using tripropylamine as alkali, to obtain an activity 1,3-dipole, performing a cycloaddition reaction, and under thealkali action of another molecule, removing molecular hydrogen bromide, to obtained a final product of isoxazole. The preparation method is capable of creatively constructing a synthetic method whichis moderate in reaction conditions, wide application range in substrate, low in requirements to apparatuses, simple and easy in acquisition of raw materials, simple in operation and high in yield.