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Guided tissue regeneration membrane containing TPE-NIM fluorescent probe and preparation method thereof

A technology that guides tissue regeneration and fluorescent probes, applied in the field of oral biomedical materials, can solve problems such as fluorescence quenching, photobleaching, and limit clinical applications, and achieve low cytotoxicity, high photostability, and no drug resistance Effect

Pending Publication Date: 2022-04-05
AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, other conventional fluorescent probes, such as rhodamine, fluorescein, BODIPY, and cyanine, have disadvantages such as fluorescence quenching caused by aggregation, and photobleaching during long-term imaging, which greatly limits their practical applications. Clinical application
Inorganic nanoparticles, such as quantum dots, have high brightness and photostability, but the potential toxicity caused by heavy metal content also affects its clinical application.

Method used

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  • Guided tissue regeneration membrane containing TPE-NIM fluorescent probe and preparation method thereof
  • Guided tissue regeneration membrane containing TPE-NIM fluorescent probe and preparation method thereof
  • Guided tissue regeneration membrane containing TPE-NIM fluorescent probe and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A preparation method of TPE-NIM fluorescent probe, comprising the following steps:

[0044] 1) Synthesis of TPE-Br: Dissolve 2.5g of 4-bromo-phenylbenzophenone and 1.8g of benzophenone in tetrahydrofuran, add 1.6g of zinc powder, stir and react, then add 20ml of titanium tetrachloride solution, 25 React at ℃ for 12 hours, slowly pour the mixture into ice water, stir to quench, add 50ml ethyl acetate, extract twice, filter, concentrate; use a 1:5 mixture of ethyl acetate and petroleum ether as the eluent , the crude product was purified by column chromatography and dried in vacuo to obtain TPE-Br as a light orange solid;

[0045] 2) TPE-B(OH) 2 Synthesis: Dissolve 4.0g TPE-Br in dry anhydrous tetrahydrofuran, slowly add 8mL of 1.0mol / L n-butyllithium solution dropwise, stir and react for about half an hour, add dropwise 5.0mL trimethyl borate, and react for 3 hours After that, the temperature was raised slowly, and the mixture was slowly poured into sodium bicarbonate ...

Embodiment 2

[0054] The preparation method of 45S5 bioactive glass powder, comprises the following steps:

[0055] Heat 2g of 45S5 bioactive glass until melting, immerse 1g of mesoporous silica nanoparticles with a diameter of 80-150μm and a pore size of 3μm-4μm into the molten bioactive glass, so that the molten bioactive glass covers the two Oxide the surface of silicon nanoparticles to obtain nanoparticle 45S5 bioactive glass powder with a diameter of 80-120 μm;

[0056]The resulting 45S5 bioactive glass powder includes 45% SiO 2 , 24.5% Na 2 O, 24.5% CaO and 6% P 2 o 5 .

[0057] Bioactive glass (45S5 BGs) powder of the present invention has the following advantages:

[0058] (1) The particle size is small and the specific surface area is large. The BGs used in the present invention is 45S5BGs powder made by melting method, white and tasteless, and the particle size is 80-120 μm. When it meets water, it forms an alkaline environment rapidly. The pH value is greater than 8, which ...

Embodiment 3

[0062] A preparation method for guiding tissue regeneration film, comprising the following steps:

[0063] Dissolve 76mg of PLGA (LA to GA mass ratio is 75:25, relative molecular weight M=7000-17000) powdery solid into 1ml of tetrahydrofuran by ultrasonication, then add 20mg of Example 2 to obtain 45S5BGs and 4mg of Example 1 respectively TPE-NIM, ultrasonic dispersion, evaporate about 0.5ml of tetrahydrofuran under nitrogen flow, take the remaining suspension and pour the bottom area of ​​10cm 2 The 45S5 bioactive glass-TPE-NIM fluorescent probe-poly(lactic-co-glycolic acid) guided tissue regeneration membrane can be obtained after standing and ventilating at room temperature for 24 hours in a polytetrafluoroethylene mold, and stored in a dry place at room temperature and protected from light.

[0064] Using a field emission scanning electron microscope and an EDS energy spectrometer to study and analyze the surface morphology and elemental composition of the guided tissue re...

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Abstract

The invention discloses a guided tissue regeneration membrane containing a TPE-NIM fluorescent probe and a preparation method of the guided tissue regeneration membrane. The preparation method comprises the following steps: (1) synthesizing TPE-Br; (2) synthesizing TPE-B (OH) 2; (3) synthesis of 4-bromine-N-(dimethylaminopropyl)-1, 8-naphthalimide is carried out; and (4) synthesizing the TPE-NIM. The GTR membrane which contains the slow-release antibacterial component, does not generate drug resistance and has bacterial traceability is used in a guided tissue regeneration technology (GTR), a membrane material is used as an isolation barrier, and the GTR membrane is placed at a periodontal tissue defect position to block gingival epithelium, prevent the gingival epithelium from growing along a root surface in a healing process and block fibrous connective tissue from being in contact with the surface of a tooth root, so that the healing effect of the gingival epithelium is improved. Therefore, periodontal ligament cells with the ability of forming new adhesion are guided to occupy the root surface preferentially, so that new adhesion is formed, and a growth space is provided for bone tissue regeneration.

Description

technical field [0001] The invention relates to a guiding tissue regeneration film containing TPE-NIM fluorescent probe and a preparation method thereof, belonging to the technical field of oral biomedical materials. Background technique [0002] Periodontitis, the progressive loss of periodontal bone tissue, is the number one cause of tooth loss in adults. Tooth loss caused by periodontitis seriously affects the patient's oral masticatory function, increases the risk of oral diseases, and affects their quality of life. [0003] For bone defects > 3mm caused by periodontitis, flap curettage combined with "barrier membrane" is often used in clinical treatment to achieve bone tissue regeneration around teeth or implants. Guided Tissue Regeneration (GTR), the principle of which is to use membrane material as an isolation barrier, place it in the periodontal tissue defect to block the gingival epithelium, prevent it from growing along the root surface during the healing proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D221/14C09K11/06C09K11/02G01N21/64C08L67/04C08K3/40C08K5/3417C08J5/18A61L31/16A61L31/14A61L31/06A61L31/02
Inventor 马骞卢晓林张光东赛德·米尔·赛德卫羽雯徐晶
Owner AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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