Method for screening new subset of periodontal ligament specific stem cells based on single cell sequencing and application thereof

A single-cell sequencing and periodontal ligament technology, applied in the biological field, can solve the problems that periodontal tissue regeneration and repair cannot be improved
CN112481364AActive Publication Date: 2021-03-12SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Publication Date
2021-03-12

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Abstract

The invention discloses a method for screening a new subset of periodontal ligament specific stem cells based on single cell sequencing and application thereof. The screened new subset specifically expresses FBLN2 and membrane protein CADM3, and meanwhile, the invention discloses application of the new subset of periodontal ligament specific stem cells in preparation of drugs for treating periodontal diseases. According to the invention, the subset of periodontal ligament specific stem cells is obtained through single cell sequencing, and in vitro flow sorting and cell subset performance verification are carried out through marker genes; According to the invention, the screened cell subset is beneficial to search for intervention targets of periodontal steady-state imbalance, and creates possibility for development of new drugs; periodontal ligament specific stem cells are used as seed cells to form organoids, and a scientific basis is provided for research and verification of new medical instrument materials; and the autologous / allogeneic transplantation feasibility of the periodontal ligament stem cells is further explored, and a new remodeling thought is provided for treating periodontal diseases.
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Description

technical field

[0001] The invention belongs to the biological field, and in particular relates to a method for screening a new subset of periodontal ligament-specific stem cells based on single-cell sequencing and an application thereof. Background technique

[0002] In the complex oral microenvironment, periodontal tissue is highly dynamic. Periodontal homeostasis is the biological basis for normal oral physiological functions such as mastication and pronunciation, as well as clinical treatment methods such as orthodontics and implants. The imbalance of periodontal homeostasis can lead to loss of periodontal attachment, alveolar bone destruction, and periodontal disease. Periodontal disease has the characteristics of high incidence, easy damage, and difficult recovery. It is the most common cause of tooth loss in humans. It is closely related to a variety of systemic diseases. It has been listed by the World Health Organization as a disease that endangers human health and...

Claims

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