Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Accelerant for proliferation and osteogenic differentiation of periodontal ligament stem cell

A periodontal ligament stem cell and osteogenic differentiation technology, applied in the field of periodontal ligament stem cells, can solve problems such as inability to restore periodontal support tissue, and achieve the effect of promoting osteogenic differentiation and proliferation

Active Publication Date: 2021-02-09
山东中医药大学第二附属医院
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods can only temporarily relieve the patient's symptoms, but cannot fundamentally restore the periodontal supporting tissue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Accelerant for proliferation and osteogenic differentiation of periodontal ligament stem cell
  • Accelerant for proliferation and osteogenic differentiation of periodontal ligament stem cell
  • Accelerant for proliferation and osteogenic differentiation of periodontal ligament stem cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0039] Silencing RP11-289F5.1 promotes ALPase activity in dental pulp stem cells

[0040] Since the expression of RP11-289F5.1 was gradually down-regulated during the osteogenic differentiation of dental pulp stem cells, we speculated that inhibiting the expression of RP11-289F5.1 could effectively promote the osteogenic differentiation of dental pulp stem cells.

[0041] 1. Design sh-RP11-289F5.1 and verify the suppression effect

[0042] (1) Design and synthesize shRNA of RP11-289F5.1, the sequence of sh-RP11-289F5.1 is as follows (the vector used is pENTR™ / U6):

[0043] sh-RP11-289F5.1 sequence Top Strand 5'-CACCGCTGAAGAACGCAATCCAACAGAGATGTTGGATTGCGTTTCTTCAGC-3', SEQ ID NO.6 Bottom Strand 5'-AAAAGCTGAAGAACGCAATCCAACATCTCTGTTGGATTGCGTTTCTTCAGC-3', SEQ ID NO.7

[0044] (2) Use fluorescent quantitative PCR to detect the inhibitory effect of sh-RP11-289F5.1, the experimental results are as follows figure 2 shown. It can be seen from the figure ...

Embodiment 3

[0052] Silencing RP11-289F5.1 to study its effect on the mineralization of periodontal ligament stem cells

[0053](1) The experiment was divided into three groups. The NC group was cultured with complete medium; the sh-NC group was transfected with sh-NC and cultured with osteogenic differentiation medium; the sh-RP11-289F5.1 group was transfected with RP11-289F5 .1 and use osteogenic differentiation medium for culture;

[0054] (2) Culture according to the experimental grouping in step (1). After 21 days of osteogenic differentiation, wash the cells with PBS, rinse the cells twice, and fix the cells with 4% paraformaldehyde for 30 min;

[0055] (3) Discard the fixative, rinse twice with PBS, prepare alizarin red staining solution, add 1.5ml alizarin red staining solution to each well, and incubate at room temperature for 30 minutes in the dark;

[0056] (4) Remove the alizarin red staining solution, rinse twice with deionized water, and take pictures for records.

[0057] ...

Embodiment 4

[0059] (1) The experiment was divided into three groups. The NC group was cultured with complete medium; the sh-NC group was transfected with sh-NC and cultured with osteogenic differentiation medium; the sh-RP11-289F5.1 group was transfected with RP11-289F5 .1 and use osteogenic differentiation medium for culture;

[0060] (2) After 14 days of osteogenic differentiation induction, the culture medium was removed and washed 3 times with PBS;

[0061] (3) Add cell lysate to lyse the cells, collect the lysate into a 1.5ml EP tube, ultrasonically crush and extract protein for 5min, 2s ultrasonication / 3s pause cycle;

[0062] (4) Centrifuge at 12000r / min for 20min at 4°C to collect the precipitate;

[0063] (5) Use the BCA method to measure the protein concentration, and add 5×SDS loading buffer;

[0064] (6) Configure 12% separating gel, load 20ug of sample, stacking gel 80V, 30min, separating gel 120V until bromophenol blue runs to the bottom of the separating gel;

[0065] (7...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an accelerant for proliferation and osteogenic differentiation of a periodontal ligament stem cell, and belongs to the technical field of stem cells. The accelerant discovers for the first time that the expression quantity of non-coding RNA RP11-289F5.1 in an osteogenic differentiation process of the periodontal ligament stem cell is down-regulated. Secondly, the accelerantdesigns and synthesizes the shRNA of silent RP11-289F5.1, and a result proves that the silent RP11-289F5.1 can effectively accelerate the proliferation and the osteogenic differentiation of the periodontal ligament stem cell.

Description

technical field [0001] The invention belongs to the technical field of periodontal ligament stem cells, in particular to a periodontal stem cell proliferation and osteogenic differentiation accelerator. Background technique [0002] Periodontitis is one of the common oral diseases, which is a chronic inflammatory disease of periodontal supporting tissues. Clinically, the main characteristics of patients with periodontitis are: bleeding on probing, redness and swelling of the gums, periodontal formation, and resorption of grooved bone, and eventually tooth loosening and loss. At present, the conventional treatment methods for periodontitis include basic treatment such as periodontal scaling and scaling, periodontal surgery such as periodontal flap surgery and root planing, and combined photodynamic therapy and other methods. However, these methods can only temporarily relieve the patient's symptoms, but cannot fundamentally restore the periodontal supporting tissue. [0003...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/12C12N15/113C12N5/077C12N5/0775
CPCC07K14/4705C12N5/0654C12N15/113C12N2310/14C12N5/0668C12N2501/999C12N2310/531
Inventor 杨桂梅孙玉梅颜淑云张瑜刘京群
Owner 山东中医药大学第二附属医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com