Anti-inflammatory anti-infection bacitracin fixing titanium alloy prosthesis capable of promoting osteogenic differentiation and bone mineralization and preparation method of prosthesis

A technology of osteogenic differentiation and titanium alloy, applied in prosthetics, medical science, etc., to achieve the effect of inhibiting inflammatory reactions, mild reaction conditions, and appropriate setting of reaction parameters

Inactive Publication Date: 2016-02-03
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the titanium alloy prosthesis modified by bacitracin immobilization

Method used

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  • Anti-inflammatory anti-infection bacitracin fixing titanium alloy prosthesis capable of promoting osteogenic differentiation and bone mineralization and preparation method of prosthesis
  • Anti-inflammatory anti-infection bacitracin fixing titanium alloy prosthesis capable of promoting osteogenic differentiation and bone mineralization and preparation method of prosthesis
  • Anti-inflammatory anti-infection bacitracin fixing titanium alloy prosthesis capable of promoting osteogenic differentiation and bone mineralization and preparation method of prosthesis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 Preparation of bacitracin-fixed titanium alloy prosthesis of the present invention (1)

[0045] 1. Introduction of functional functional group amino group on the surface of titanium sheet

[0046] A functional functional group amino group was introduced on the surface of titanium alloy by a two-step method. First, the titanium alloy Ti6AL4V (Shanghai Yantai Metal Materials Co., Ltd.) immersed in NaOH solution with a concentration of 5M, and reacted at 80°C for 12h. Then take out the titanium sheet and ultrasonically clean it three times with deionized water, soak it in deionized water after taking it out to remove the residual alkaline solution, and dry it. The titanium sheet was then immersed in 2 mg / ml dopamine Tris-HCl (pH=8.5) solution, reacted at 30° C. for 8 h, ultrasonically cleaned three times with deionized water, and dried.

[0047] 2. Covalent bond immobilization of bacitracin on the surface of titanium sheet

[0048] Put the titanium sheet ...

Embodiment 2

[0067] Embodiment 2 Preparation of bacitracin-fixed titanium alloy prosthesis of the present invention (two)

[0068] 1. Introduction of functional functional group amino group on the surface of titanium sheet

[0069] A functional functional group amino group was introduced on the surface of titanium alloy by a two-step method. Firstly, the titanium alloy disc was immersed in NaOH solution with a concentration of 10M, and reacted at 60°C for 10h. Then take out the titanium sheet and ultrasonically clean it three times with deionized water, soak it in deionized water after taking it out to remove the residual alkaline solution, and dry it. The titanium sheet was then immersed in a 5 mg / ml dopamine Tris-HCl (pH=9) solution, reacted at 25° C. for 12 hours, ultrasonically cleaned three times with deionized water, and dried.

[0070] 2. Covalent bond immobilization of bacitracin on the surface of titanium sheet

[0071] Put the titanium sheet introduced with the functional func...

Embodiment 3

[0071] Put the titanium sheet introduced with the functional functional group amino into the acetic acid solution of bacitracin (the concentration of bacitracin is 5 mg / ml, prepared with 5 μM acetic acid), pH=3, add 1-(3-dimethylaminopropyl)- 3-Ethylcarbodiimide (EDC) aqueous solution and N-hydroxysuccinimide (NHS) aqueous solution, so that the final concentration of EDC is 2.5 mg / mL, and the final concentration of NHS is 0.35 mg / mL, under inert gas nitrogen reflux The reaction was terminated after 6 hours of protection reaction, and the temperature during the reaction was 37°C. Ultrasonic cleaning with deionized water and vacuum drying. Embodiment 3 Preparation of bacitracin-fixed titanium alloy prosthesis of the present invention (three)

[0072] 1. Introduction of functional functional group amino group on the surface of titanium sheet

[0073]A functional functional group amino group was introduced on the surface of titanium alloy by a two-step method. Firstly, the tita...

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Abstract

The invention relates to an anti-inflammatory anti-infection bacitracin fixing titanium alloy prosthesis capable of promoting osteogenic differentiation and bone mineralization and a preparation method of the prosthesis. According to the preparation method of the prosthesis, bacitracin is fixed on titanium alloy through a covalent grafting method, and an obtained bacitracin modified titanium sheet can keep antibacterial biological activity and can promote mesenchymal stem cell osteogenic differentiation and bone mineralization, in addition, a function of inhibiting inflammatory factor secretion is also realized, and moreover, the preparation process is simple, the reaction condition is mild, the efficiency is high, the cost is low, and the repeatability is good. The anti-inflammatory anti-infection bacitracin fixing titanium alloy prosthesis capable of promoting osteogenic differentiation and bone mineralization provided by the invention can effectively improve the defect of the single biological function of the titanium alloy applied clinically at present, and reduce the related complications such as the infection after the titanium alloy is implanted into a body and the looseness caused by excessive inflammatory reaction in an early stage.

Description

technical field [0001] The invention relates to the technical field of orthopedic prosthesis, in particular to a bacitracin-fixed titanium alloy prosthesis for anti-inflammation, anti-infection and promotion of osteogenic differentiation and bone mineralization and a preparation method thereof. Background technique [0002] A perfect orthopedic implant should avoid complications after implantation. Zhao (Zhao, Journal of biomedical materials research Part B, Applied biomaterials 2009, 91 (1): 470-480) proposed that antibacterial coating is an important means to improve the infection of internal plants. Zhang (Zhang, IntJMolSci2014, 15(7): 11878-11921.) believes that implants that promote osseointegration and resist infection have the ability to reduce the risk of aseptic and septic loosening. Goodman (GoodmanSB, Biomaterials2013, 34(13):3174-3183) proposed that the future focus of orthopedic biocoating implants should be to improve osseointegration, reduce infection, and re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/06A61L27/54
Inventor 岳冰聂彬恩敖海勇
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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