Scaffold material for in vitro osteogenesis induced differentiation of mesenchymal stem cells, and application of scaffold material

Abstract

The invention discloses a scaffold material for in vitro osteogenesis induced differentiation of mesenchymal stem cells, and application of the scaffold material. The scaffold material is characterized in that a porous silk protein scaffold is taken as a main body, the scaffold is enveloped with organic silicon by means of biological silicification, and the porous silk protein scaffold enveloped with the organic silicon is taken as a culture carrier for the in vitro osteogenesis induced differentiation of human marrow mesenchymal stem cells; the human marrow mesenchymal stem cells are taken as seed cells and are inoculated onto the porous silk protein scaffold enveloped with the organic silicon according to certain cell density, and the porous silk protein scaffold enveloped with the organic silicon is placed in an osteogenesis induced differentiation micro-environment for culturing. A three-dimensional osteogenesis induced differentiation system established by using the scaffold material not only can be used for directionally inducing the human marrow mesenchymal stem cells to differentiate into osteoblasts in bone tissue engineering so as to enable the osteoblasts with stable traits to be more efficiently obtained, but also can provide important theoretical guidance for the research of the bone tissue engineering so as to provide new ideas for the clinical treatment of osteoporosis, bone injury and other orthopedic diseases.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a scaffold material for culturing human bone marrow mesenchymal stem cells in vitro and its application. Background technique [0002] Bone marrow mesenchymal stem cells have become the most important seed cells in the field of bone tissue engineering research because they are easy to obtain, easy to culture and proliferate in vitro, and have good osteogenic, chondrogenic, adipogenic and even myogenic cell differentiation potentials, and have a wide range of applications prospect. [0003] Targeted induction of human bone marrow mesenchymal stem cells to differentiate into osteoblasts provides important theoretical guidance for the study of bone tissue engineering, and provides new ideas for the clinical treatment of osteoporosis, bone injury and other orthopedic diseases. [0004] Many progresses have been made in the research on the induction of specific osteo...

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Problems solved by technology

[0004] Many progresses have been made in the research on the induction of specific osteogenic differentiation of BMSCs, and a variety of scaffold materials have been successfully used for osteogenic induction and differentiation. However, most of the scaffold materials with high mechanical strength that can promote osteogenic differentiation are synthetic materials, which are difficult to form An ideal material-cell interface affects the adhesion, proliferation, and differentiation of seed cells in the material. At the same time, the contradiction between the mechanical strength and degradation speed of the material cannot be resolved and the material still has a certain antigenicity in the body; it is more conducive to cell Growth-added natural scaffold materials have poor plasticity and weak mechanical strength

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