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Method for extracting natural bioactive protein from unpolished rice or/and rice bran

A technology for biologically active proteins and salt-soluble proteins is applied in the field of extracting natural biologically active proteins, which can solve the problems of complex and uncertain components, insufficient cleanliness, complicated processes, etc., and achieves good economic and environmental benefits, low preparation cost, The effect of inhibiting lipid peroxidation

Inactive Publication Date: 2015-04-22
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As mentioned above, the main defects of the existing rice active peptide technology are: first, the preparation of rice protein mostly involves acid and alkali treatment, and the resulting waste water may pollute the environment, and the process is not clean enough; Active peptides are obtained by enzymatic hydrolysis steps, which are costly and complicated; third, the prepared active peptides are not naturally present in rice, but are obtained by enzymatic hydrolysis of rice protein, and the components are complex and uncertain

Method used

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  • Method for extracting natural bioactive protein from unpolished rice or/and rice bran
  • Method for extracting natural bioactive protein from unpolished rice or/and rice bran

Examples

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Effect test

Embodiment 1

[0028] 1) The commercially available Beidahuang Lvye organic brown rice from Beidahuang Green Food Distribution Co., Ltd. was used as the raw material, which was crushed and passed through a 40-mesh sieve to obtain the rice flour raw material. Acetone was used to degrease the rice flour raw material. The rice flour raw material (g) was mixed with acetone ( mL) Solid-to-liquid ratio 1:3g / mL, shake for 2 hours, centrifuge at 6000rpm for 20 minutes to collect the precipitate, and place it in a fume hood to dry naturally to obtain defatted rice noodles.

[0029] 2) Extraction of total salt-soluble protein from rice: Weigh 15g of defatted rice flour and add 120mL of 1.0mol / L NaCl aqueous solution. supernatant to obtain a total salt-soluble protein solution;

[0030] 3) Ethanol precipitation to remove impurities: add absolute ethanol to the total salt-soluble protein solution in step 2), the volume ratio of the total salt-soluble protein solution to absolute ethanol is 1:2, and stir...

Embodiment 2

[0033] 1) The commercially available Beidahuang Green Field organic brown rice from Beidahuang Green Food Distribution Co., Ltd. was used as the raw material, which was crushed and passed through a 50-mesh sieve to obtain the rice flour raw material. Acetone was used to degrease the rice flour raw material. The rice flour raw material (g) was mixed with acetone ( mL) solid-to-liquid ratio 1:3g / mL, after shaking for 2h, centrifuge at 6000rpm for 20min to collect the precipitate, and place it in a fume hood to dry naturally to obtain defatted rice noodles;

[0034] 2) Extraction of total salt-soluble protein from rice: Weigh 15g of defatted rice flour and add 150mL of 1.0mol / L NaCl aqueous solution, mix well, shake and extract at 30°C for 2h, centrifuge at 6000rpm for 20min to collect the supernatant, repeat step 2) once, and combine the supernatant solution to obtain the total salt-soluble protein solution;

[0035] 3) Ethanol precipitation to remove impurities: add absolute et...

Embodiment 3

[0038] 1) Use commercially available fine defatted rice bran powder produced by Jiangxi Tianyu Oil Co., Ltd. as raw material, as defatted rice flour;

[0039] 2) Extraction of total rice salt-soluble protein: Accurately weigh 15g defatted rice flour and add 1.0mol / L NaCl aqueous solution 150mL, mix well, shake and extract at 30°C for 2h, centrifuge at 6000rpm for 20min to collect the supernatant, repeat step 2) once, combine supernatant to obtain a total salt-soluble protein solution;

[0040] 3) Ethanol precipitation to remove impurities: add absolute ethanol to the total salt-soluble protein solution in step 2), the volume ratio of the total salt-soluble protein solution to absolute ethanol is 1:2.5, and stir and mix with a magnetic stirrer at the same time, place Precipitate at 4°C for 1 hour, centrifuge at 8000rpm for 20 minutes, remove the precipitated part, collect the supernatant to obtain the biologically active protein solution;

[0041] 4) Post-treatment, ethanol re...

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Abstract

The invention discloses a method for extracting natural bioactive protein from unpolished rice or / and rice bran. The method comprises the followings: processing unpolished rice or / and rice bran to obtain degreased rice flour; mixing degreased rice flour and soduim chloride water, then extracting through oscillation, and collecting supernatant fluid through first separation, namely total salt soluble protein solution is obtained; adding alcohol into the total soluble protein solution, mixing uniformly, and removing precipitation part through standing and second separation so as to obtain bioactive protein solution; removing alcohol and salinity, and drying through post-processing to the bioactive protein solution, and then obtaining the natural bioactive protein. The method provided by the invention adopts two food-grade raw materials of sodium chloride and alcohol, and can be used for extracting the natural bioactive protein from the unpolished rice or / and rice bran, the cost is low, and zero contamination is achieved; the natural bioactive protein prepared by the invention can be used for preparing health foods, taken as food additives and applied to cosmetics, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of bioactive protein preparation, in particular to a method for extracting natural bioactive protein from brown rice or / and rice bran. Background technique [0002] Rice protein is an edible protein with high nutritional value, mainly due to its reasonable amino acid composition, high utilization rate and biological potency, and hypoallergenicity. It is the only grain protein that can be exempted from allergy experiments (Wang Zhangcun et al. .Research progress of rice protein. Chinese Journal of Cereals and Oils, 2004, 19(2): 11-17). According to Osborne's solubility-based classification method, rice proteins can be roughly classified into albumins, globulins, gliadins, and glutenins. Among them, albumin and globulin are protein components in rice that can be dissolved and extracted with sodium chloride, also known as salt-soluble proteins, and are the main physiologically active proteins. The reported physiological...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/30C07K1/14
Inventor 赵艳
Owner ZHEJIANG GONGSHANG UNIVERSITY
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