8results about How to "Non-cytotoxic" patented technology

The invention discloses a medicine for treating osteoarthritis, and belongs to the field of medicine, the medicine comprises a reagent capable of stimulating chondrocyte development, and the medicine L-arginine can promote expression of chondrocyte anabolic factors and reduce expression of cartilage degradation markers, so that chondrocytes are protected to a certain extent; in addition, the medicine provided by the invention has an excellent cartilage repair function, can inhibit the progress of degenerative osteoarthritis, has good biocompatibility, and has no cytotoxicity to cartilage cells.

The present application relates to the technical field of biology, and particularly discloses a polypeptide, a recombinant humanized collagen of type XIX and application thereof. The amino acid sequence of the polypeptide comprises n repeating units; the amino acid sequence of the repeating unit is shown in SEQ ID NO. 2. The polypeptide has a collagen triple helix structure, has good cell adhesion activity, can perform the function of human collagen, has no immune rejection and allergic reaction, and can be used in various fields such as cartilage repair, biological dressing, tissue filling and the like.

Disclosed are ketone amide derivatives and applications thereof, and specifically disclosed are compounds of formula (IV) and pharmaceutically acceptable salts thereof.

This invention discloses an antimicrobial peptide, Cih1, and its applications. The amino acid sequence of antimicrobial peptide Cih1 is shown in SEQ ID NO.1. The antimicrobial peptide Cih1 provided by this invention is a cationic peptide, rich in cysteine ​​(-cys) residues, which can form an amphiphilic β-sheet secondary structure. This structure endows it with excellent antibacterial and antiviral activity, and can effectively inhibit the proliferation and invasion of pathogens in aquaculture water. Based on the broad-spectrum resistance of antimicrobial peptide Cih1 to pathogens, it can help aquatic animal hosts resist infection by various pathogens, and has broad application prospects in the field of anti-infective drug development for aquaculture.

Disclosed are ketone amide derivatives and applications thereof, and specifically disclosed are compounds of formula (IV) and pharmaceutically acceptable salts thereof.

This invention discloses a method for preparing soluble regenerated silk fibroin lyophilized powder with controllable molecular weight, yielding a lyophilized powder that exists primarily in a SilkⅠ aggregated state and can be rapidly and completely dissolved. By optimizing the degumming, dissolution, dialysis purification, and lyophilization processes, combined with clarification filtration and low-temperature control, this invention overcomes the stability and quality problems of existing soluble regenerated silk fibroin preparation methods. By controlling key production process parameters, it is possible to prepare regenerated silk fibroin lyophilized powders with different molecular weight ranges for application in the preparation of biodegradable medical materials with varying properties.

The invention discloses a complex microbial inoculant as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The complex microbial inoculant disclosed by the invention is prepared by co-fermentation of lactobacillus plantarum CGMCC (China General Microbiological Culture Collection Center) NO.12849 and enterococcus faecium CGMCC The complex microbial inoculant has the characteristics of high antibacterial activity, safety, no toxicity, high stability and the like, and can be used for killing clinically common gram-negative pathogenic bacteria. By utilizing the high-density fermentation culture medium, the fermentation process and the preparation process provided by the invention, the complex microbial inoculant can be produced on a large scale and is low in cost, and the complex microbial inoculant is expected to replace antibiotics to become a safe, green and efficient ideal antibacterial agent and has a very wide application prospect.

The present application provides a kind of polypeptide sustained-release hydrogel coating for preventing catheter urinary tract infection and its preparation method, the coating includes the adhesive bottom layer attached to the surface of catheter substrate, and the polypeptide sustained-release hydrogel functional layer compounded outside the adhesive bottom layer;The functional layer includes hydrophilic hydrogel gel-forming matrix, targeted antibacterial polypeptide, polypeptide sustained-release carrier and biocompatible auxiliary agent.The present application solves the interface bonding problem of hydrophobic catheter substrate and hydrophilic hydrogel through the adhesive bottom layer, the coating has strong adhesion, is resistant to urine immersion and rubbing;Through polypeptide sustained-release carrier, antibacterial polypeptide is released for 30 days, and there is no risk of burst release;The targeted antibacterial polypeptide used is specific to urinary tract infection pathogenic bacteria, has no drug resistance induction risk, has excellent biocompatibility, can effectively prevent catheter-related urinary tract infection caused by short-term and long-term indwelling catheter, the preparation process is simple and controllable, is suitable for large-scale production, and can be widely applied to antibacterial modification of urinary intervention medical devices.