603results about How to "Non-cytotoxic" patented technology

The invention relates to the high degree of exchange Ca-A type zeolite hemostasis dressing and the preparation method and the purpose. The zeolite hemostasis dressing of the invention containing the zeolite has fast hemostasis speed, and has no bacterium, no pyrogen, no cell toxicity, no hypersensitive reaction and no skin irritation, the using is convenient and the cost is low.

The invention discloses biological ink for 3D printing. The biological ink for 3D printing comprises water-soluble synthetic polymer with the cross-linking function, water-soluble natural polymer with the cross-linking function, bioactive components capable of spontaneously forming special ultrastructures, cross-linking initiator and solvents and further comprises bioactive components. The biological ink for 3D printing overcomes the defects that traditional 3d printing ink is single in component structure, does not have good biological activity and needs to utilize organic solvents, and it is difficult to take cell compatibility, biological activity and mechanical properties into consideration, the biological ink which has biological activity and the fast curing function at the same time and can form a certain microstructure is obtained, and hydrogel obtained through curing of the ink has controllable mechanical properties, good structural stability and a good fidelity effect.

The invention discloses a method for preparing oligomeric hyalurate by a digestion method, and the oligomeric hyalurate and an application thereof. Bacillus hyaluronidase obtained through fermental cultivation of Bacillus sp. A50 CGMCC NO.5744 is used for degrading hyaluronic acid or a salt thereof, and the method comprises the steps of preparing the hyaluronic acid or the salt thereof, enzymolysis, inactivation, filtering, settling, dewatering and drying. According to the method, the hyaluronidase produced by the Bacillus is used for degrading the hyaluronic acid or the salt thereof, and the oligomeric hyalurate is prepared through dezymotizing, alcohol or ketone settling and dewatering and drying. The method is simple to operate and mild in condition, has no destroy on the product structure and no environmental pollution, the hyaluronidase for fermentation is low in cost and is suitable for large-scale industrial production, the prepared oligomeric hyalurate has the advantages of good percutaneous absorption capability, high purity, no cytotoxicity, strong oxidation resistance and the like, and can be used in fields such as cosmetics, foods and medicines.

The invention discloses a hyperglycosylated human coagulation factor VIII (FVIII) fusion protein and a preparation method and application thereof. The fusion protein successively contains human FVIII, a soft peptide connector, at least one human chorionic gonadotropin betasubunitcarboxyl terminal peptide rigid unit and prolonged half-life period portion (preferably ahumanIgG Fc variant) from an end N to an end C. The fusion protein has biological activity similar to that of recombinant FVIII and prolonged in-vivo activity half-life period, thereby improving the pharmacokinetics and drug efficacy.

The invention discloses a graphene oxide / polymer composite antibacterial material and a preparation method thereof. The composite antibacterial material is characterized in that a polymer is subjected to surface modification and then is combined with graphene oxide dispersed in water, thus obtaining a graphene oxide antibacterial coating bonded on a polymer matrix, wherein the thickness of the antibacterial coating is 1 to 50nm. The preparation method of the graphene oxide / polymer composite antibacterial material comprises the following steps: firstly, performing ultrasonic cleaning on the polymer; secondly, performing surface modification on the polymer subjected to ultrasonic cleaning; thirdly, immersing an activated polymer matrix into a coupling agent solution and soaking; fourthly, taking out the soaked polymer, leaching with deionized water, then putting into a graphene oxide suspension liquid for 0.2 to 24 hours, taking out, leaching and naturally drying in air to obtain the graphene oxide / polymer composite antibacterial material. The invention designs and prepares the composite antibacterial material by taking graphene oxide as an antibacterial ingredient; the composite antibacterial material has a transparent antibacterial layer, is excellent in antibacterial property and is firmly bonded with the matrix.

The invention discloses a green mud crab antibacterial peptide Sphistin and application thereof, relates to the technical field of genetic engineering of crustaceans, and provides the green mud crab antibacterial peptide Sphistin and the application thereof. A molecular formula of the green mud crab antibacterial peptide Sphistin is C170H293N61O46S1 and molecular weight of the green mud crab antibacterial peptide Sphistin is 3,959.61 Dalton. The antibacterial peptide Sphistin consists of 38 amino acids, is derived from a part of sequences at end N of green mud crab histone 2A protein and is apolypeptide with an antibacterial function. By adopting the conventional solid-phase chemical synthesis method, the green mud crab antibacterial peptide Sphistin with purity of over 95 percent can beobtained. The green mud crab antibacterial peptide Sphistin has an obvious antibacterial effect on various gram negative bacteria and gram positive bacteria, has a broad antibacterial spectrum and high sterilization rate, shows extremely high medical value and is excellently applied to preparing antibacterial agents.

The invention belongs to the fields of enzymology and pharmaceutical chemistry, and relates to the low molecular hyaluronate, preparation method and purpose thereof. Concretely, the preparation method comprises a degradation step of hyaluronic acid or salt thereof whose molecular weight is greater than 1000 kDa by hyaluronidase prepared by Bacillus spp whose preservation number is CGMCC No. 5744 or SEQ ID No:2 coded hyaluronidase. The preparation of low molecular hyaluronic acid or salts thereof by using hyaluronidase produced by Bacillus spp of the present invention has the advantages of simple operation, mild condition, non-destroy of the product structure and low cost. The product is suitable for large scale industrialized production. The low molecular hyaluronate prepared by the invention has the advantages of good transdermal absorbency, good purity, non-cytotoxicity, good antioxidation, etc., and can be used in the fields of cosmetic, foodstuff and medicine.

The invention provides a double-layered composite biological dressing and an adhesive-free compound technology thereof. The double-layered composite dressing comprises a chitosan fiber non-woven fabric layer and a spongy-shaped functional layer, wherein no adhesive is needed to bond the two layers. The preparation technology comprises the following steps: (1) chitosan fiber non-woven fabric is pre-cooled; (2) macromolecule chitosan, macromolecule collagen protein, micromolecule collagen protein and micromolecule chitosan in mass ratio of 0.7-1.3 to 0.1-0.3 to 0.7-1.3 to 0.1-0.3 are added in a faintly acid aqueous solution to prepare a spongy layer material solution; (3) the pre-cooled chitosan non-woven fabric is taken out and the spongy layer material solution is rapidly and uniformly sprayed on the non-woven fabric, wherein the spraying amount is 20-60 ml / 20*20 cm<2>; (4) the chitosan non-woven fabric sprayed with the spongy layer material solution is freeze-dried. The prepared double-layered composite dressing is firm in embedding connection between the two layers; the spongy functional layer has the function of promoting wound healing and prevents adhesion; the chitosan fiber non-woven fabric layer has the functions of favorable air permeability, comfort and bacterium resistance.

The invention discloses a preparation method of an amino-enriched polymeric thin film. A double-terminal amino compound and a catechol compound are copolymerized by Michael addition and Schiff alkali reaction, so as to form a film with abundant amino functional groups. Regulation and control on the density of the surface amino functional group can be achieved by regulating and controlling the conditions such as reaction time, reactant concentration, oxygen concentration, the temperature and the like. A target film surface not only contains abundant amino functional groups, is high in intermolecular crosslinking degree, and good in film stability, and provides abundant reaction sites for different types of biological material surface-grafted biological molecules, but also has good biocompatibility on vascular cells.

The invention discloses a preparation method of the acellular dried active amnion, comprising the following steps: (1) material processing: taking a human placenta which passes the detection of pathogen and obtained from a full term cesarean section, washing the human placenta with aseptic normal saline, removing blood components from the human placenta, blunt dissecting the amnion from the human placenta perfectly, striking off matrix components from the amnion substrate with a cell curet; (2) repeatly freezing and thawing; (3) washing; (4) freeze drying; and (5) disinfecting and sterilizing with gamma-ray. The method adopts the combination of repeat freeze-thaw method and using decontaminating agent to remove amnion cells, overcomes the defects of bad effects of removing cells by using freeze-thaw method alone, removes amnion cells effectively, keeps the integrity of collagen structure and basement membrane in tissue well, and keeps good mechanical property and no cytotoxicity.

The invention provides an ultrasound gel. The ultrasound gel contains the following components in percentage by weight: 0.1 to 10 percent of glycosaminoglycan, and 5 to 15 percent of glycerol. The ultrasound gel has the characteristics of high-efficiency disinfection and sterilization, no cytotoxicity, no skin allergy and no irritation.

The invention discloses a preparation method of a nano collagen membrane. The method includes the following steps of step one, dissolving collagen in hexafluoroisopropanol to obtain an 80-120mg / ml of collagen hexafluoroisopropanol solution; and step two, subjecting the collagen hexafluoroisopropanol solution obtained from the step one to electrostatic spinning with voltage of 15-19 KV, the receiving distance of 12.5-15cm and the advance speed of 0.5-1.2 ml / h so as to remove hexafluoroisopropanol to obtain the nano collagen membrane. The invention further discloses a preparation method of a double-layer collagen membrane, the prepared collagen membrane and application of the collagen membrane. The collagen membrane is excellent in mechanical property, free of cytotoxicity, low in hemolysis and antigenicity and high in biological activity.

The invention provides a premixed calcium silicate based root canal filling material with suspension stability as well as a preparation method and an application thereof. At least one calcium silicate compound is used as a main phase, and at least a second phase for improving solidification performance, at least one nonaqueous phase solvent which can be mixed with water, at least one suspension stabilizing agent and at least one radiopaque material are added in order to prepare the root canal filling material with excellent injectable performance. An inorganic dispersant with suspension stabilizing effect is added into the premixed type calcium silicate based root canal filling material system, based on reservation of excellent biological compatibility, biological activity, marginal sealability, and the like of the calcium silicate material, layering, deposition or flocculation of the premixed system are effectively prevented, long-term dispersion stability of the premixed paste is improved, and the material can be used as a filling material for dental pulp capping, root canal therapy, and other dental restoration fields.

The invention relates to a bone repair material containing nano hydroxyapatite / collagen particles, and a preparation method thereof. The invention provides a bone repair material which comprises: 1 to 2 parts by weight of the nano hydroxyapatite / collagen particles, 1 part by weight of chitosan and 2 to 3 parts by weight of Beta-sodium glycerophosphate (which is supplemented by the weight proportion of the components). The invention also provides a preparation method of the bone repair material; the bone repair material is placed at 37 DEG C or injected into human body, thus forming in-situ gelatin. The bone repair material provided by the invention not only can retain the characteristics of modeled natural bone structure, but also ensures the new performance of syringeability of the material and has temperature sensitivity, thus being applicable to minimal invasive surgery and convenient for clinical application.

The present invention provides a composite nano antimicrobial material and its preparation method. It includes nano titanium oxide and nano silver system antimicrobial material. The average grain size of said nano material granules is 5-100nm, and the weight ratio of main component is as follows: nano zinc oxide: nano titanium oxide: nano silver system antimicrobial material; nano silver-copper system antimicrobial material=1:0.1-0.5:0.5-1.5:1.5-3.5. Said invention also provides the concrete steps of its preparation method.

The invention discloses a preparation method of a human skin stem cell factor nano-liposome-exosome complex. According to the method, the exosome and the liposome are compounded, and through the synergistic effect between the exosome and the nano-liposome coated with the cytokine, the obtained compound system has good biocompatibility and immune escape performance, is free of cytotoxicity, not prone to being degraded by macrophages, good in stability and capable of continuously playing a role in vivo.

The invention discloses a preparation method of a carbon nanotube reinforced hydroxyapatite composite material, relating to a composite material for prosthesis materials. According to the preparation method, a carbon nanotube is synthesized in hydroxyapatite powder, the carbon nanotube is subjected to surface modification by using hydroxyapatite, and the carbon nanotube reinforced hydroxyapatite composite material is further prepared. The preparation method comprises the following steps of: firstly preparing carbon nanotube-hydroxyapatite powder from nickel carbonate and the hydroxyapatite powder, then preparing hydroxyapatite-modified carbon nanotube-hydroxyapatite powder, and finally preparing the carbon nanotube reinforced hydroxyapatite composite material. The preparation method disclosed by the invention overcomes the defects that the carbon nanotube is difficult to disperse in a hydroxyapatite matrix, the wettability and the interfacial strength between the carbon nanotube and the hydroxyapatite matrix are low, the hydroxyapatite powder of which the surface is loaded with the carbon nanotube is difficult to form and the biocompatibility of the composite material is poorer in the prior art.

The invention belongs to the technical field of medical dressing, and discloses a carbon quantum dot / nano-silver alginate dressing and a preparation method and application thereof. A carbon quantum dot / nano-silver composite material is prepared from silver nitrate, polyethylene glycol 400, sodium citrate, ascorbic acid and carbon quantum dots; and the carbon quantum dot / nano-silver composite material is added to a sodium alginate solution to prepare the carbon quantum dot / nano-silver alginate dressing through an electrostatic spinning technology and a non-woven technology. According to the dressing prepared by the technology, the mechanical property of the alginate dressing can be significantly improved; meanwhile, the dressing has an excellent fluorescent display tracking function; the migration change and the accumulation condition of silver ions in a body can be effectively inspected; and a fast and effective detection method is provided for clinical supervision of the silver ions.

The invention belongs to the technical field of natural polymer materials and particularly discloses bi-network high-intensity fibroin hydrogel and a preparation method thereof. Fibroin hydrogel subjected to chemical crosslinking (a first network) of horse radish peroxidase is soaked in a solution which can induce fibroin beta to fold and transform, physical crosslinking (a second network) is formed, and then the bi-network high-intensity fibroin hydrogel with the solid content being 2-20 wt% can be prepared. The hydrogel is good in elasticity, high in intensity and easy to regulate and has biocompatibility, the performance is stable under the condition of the simulated in-vivo environment, and the bi-network high-intensity fibroin hydrogel can be widely used for fields of drug sustained release carriers, cartilage tissue, ligament tissue and other biomedicine and regeneration medicine engineering.

The invention discloses a method for preparing a hyaluronate oligomer according to a digestion method, a prepared hyaluronate oligomer and an application thereof. Bacillussp. A50 CGMCC NO.5744 is fermented and cultured, thereby obtaining Bacillussp hyaluronidase for degrading hyaluronic acid or salt solution thereof. The method comprises the following steps of: preparing a solution of the hyaluronic acid or salt solution thereof; performing enzymolysis; inactivating; filtering; depositing; and dewatering and drying. According to the invention, the Bacillussp hyaluronidase generated by the Bacillussp is utilized to degrade the hyaluronic acid or salt thereof; the hyaluronate oligomer is prepared according to the steps of dezymotizing, ethanol precipitation, dewatering and drying; the method is simple in operation, mild in condition, free from damage to product structure and free from environmental pollution; the cost of the hyaluronidase as a fermentation source is low; the method is suitable for large-scale industrial production; the prepared hyaluronate oligomer has the advantages of excellent transdermal absorption property, high purity, zero cytotoxicity, strong oxidation resistance, and the like; and the hyaluronate oligomer can be applied to the fields of cosmetics, food and medicines.

The invention discloses a preparation method of medical temperature-sensitive hydrogel. The preparation method comprises the following steps: taking carboxymethyl chitosan, polyvinyl alcohol, sodium alginate and silver nitrate as raw materials; dissolving and mixing; then adding an ascorbic acid water solution and glutaraldehyde; obtaining crude gel by adopting a microwave radiation method; washing the crude gel with diluted acetic acid and water; drying the crude gel in vacuum at 40 DEG C until the weight is constant, so as to obtain the medical temperature-sensitive hydrogel. The medical temperature-sensitive hydrogel disclosed by the invention has a relatively good antibacterial effect, has a wide antibiotic spectrum and no cell toxicity and has high efficiency and low toxin; after the medical temperature-sensitive hydrogel is uniformly sprayed, one layer of a gel thin film is formed on the surface of a wound and has a lasting antibacterial effect and good air permeability and moisture retention; the medical temperature-sensitive hydrogel has the effects of promoting wound healing, stopping bleeding, stopping pain, resisting inflammation and sterilizing, can be used for large-area irregular traumas including burns, scalds, traumas and the like and can also be used for other skin inflammations including bedsores, skin ulcers, tinea manus and pedis, female vaginitis, cervicitis, cervical erosion and the like.

The invention discloses a vascular stent coating with a drug sustained-release function, which is prepared from the following raw materials: polycaprolactone, chitosan, collagen, medicine, nano magnesium, nano titanium dioxide, dendrobium polysaccharide, tea polyphenol, dopamine, gelatin, nano zirconia, hirudin, and ethanol. The invention also discloses a preparation method of the vascular stent coating with the drug sustained-release function. The binding strength of the vascular stent coating and a substrate is 4.8 to 5.9 MPa, the binding strength of the vascular stent coating and the stent substrate is high, the degradation time of the vascular stent coating is 68 to 86 days, the vascular stent coating can be completely degraded after 90 days, the large-area shedding and swelling phenomena do not occur in the degradation process, the stability and biocompatibility are good, no cell toxicity, coagulation and thrombosis are formed, and no inflammatory response is generated.

The invention discloses aquagel, a preparation method therefor and an application of the aquagel. The aquagel is a product obtained through subjecting an O-quaternary ammonium salt chitosan solution and an aldehyde-group sodium alginate solution to a mixed reaction. Simultaneously, the invention further discloses the preparation method for the aquagel. The method comprises the following steps: 1)modifying chitin to prepare O-quaternary ammonium salt chitosan; 2) modifying sodium alginate to prepare aldehyde-group sodium alginate; and 3) preparing the aquagel. The invention further discloses the application of the aquagel. The aquagel disclosed by the invention has bacterial inhibition, moisture preserving property and high biocompatibility and has a broad application prospect in the fields of biomedicine, reshaping facial beautification and the like. Aquagel products can serve as wound surface dressing in medical clinics and products of skin repairing, nursing and the like of reshaping facial beautification.

A polylysine-starch nanoparticle composed of the anionic starch nanocore and the cationic polylysine shell layer is prepared through adding the hydrolytic starch liquid to oil phase, stirring, addingcross-linking agent, reaction while stirring, washing with alcohol, drying to obtain said nanocores, dispersing them in buffering phosphoric acid solution, adding the aqueous solution of polylysine, waving and centrifugal separation. It can be used as gene carrier with advantages of high transduction efficiency, no immunogenicity, no cell toxic, and good biodegradability.

The invention discloses an artificial bone containing hydroxyapatite whiskers and diphasic calcium phosphate and a preparation method of the artificial bone. The hydroxyapatite whiskers, hydroxyapatite powder and beta-tricalcium phosphate are used as raw materials of the artificial bone, the artificial bone has an induction effect on growth of osteocytes, and growth and healing of the osteocytes are promoted; by controlling the ratio of the hydroxyapatite whiskers, the hydroxyapatite powder and the beta-tricalcium phosphate, the strength of the artificial bone is increased; the preparation method of the artificial bone is a 3D printing technology, on the one hand, the 3D printing technology can customize a stent according to the shape of a bone defect portion, and the shortcomings that bymanufacturing through a traditional mold, molding is required at first, then size is limited after molding, and the cost of the mold is high are overcome; and on the other hand, by the method, the aperture, the porosity and the connectivity of the stent can be controlled precisely, and an open porous structure and an interconnected network are of great important on cytotrophy supply, proliferationand differentiation and formation of tissues and blood vessels.

The invention discloses an injectable articular cartilage tissue repair material and is characterized in that starting materials of the composite material contain the following components of: by weight, 100 parts of I-type collagen and 10-50 parts of hyaluronic acid, wherein oxidisability of hyaluronic acid is 20-60%. A monocyte layer in bone marrow fluid in vivo is embedded in the repair material. The thickness of the composite material is 1-5mm. Hyaluronic acid is oxidized into oxidized hyaluronic acid by the use of sodium periodate. The collagen solution, 5*PBS and a buffer are mixed at the volume ratio of 7:2:1 and the pH is adjusted to neutral. After dissolving oxidized hyaluronic acid by the use of ultrapure water, the dissolved oxidized hyaluronic acid is added into the neutral collagen solution and the mixed solution is uniformly stirred and stands in a refrigerator of 4 DEG C. The separated and extracted monocyte layer in the bone marrow fluid by a density gradient centrifugation method is uniformly mixed with the above collagen-oxidized hyaluronic acid solution. The mixed liquor is putted into a constant temperature incubator of 37 DEG C for standing or injection to the organism articular cartilage defects so as to form the composite material.

The invention relates to a graphene anti-bacterial compound solution and a preparation method thereof. The graphene anti-bacterial compound solution is prepared from the following components in percentage by weight: 5 to 20 percent of an anionic surfactant, 3 to 15 percent of a non-ionic surfactant, 5 to 10 percent of an anti-redeposition component, 1 to 10 percent of a thickening agent, 1 to 15 percent of an emulsifying agent, 0.1 to 1 percent of graphene powder, 0.1 to 0.7 percent of pigment, 0.1 to 0.5 percent of perfume and the balance of water. According to the graphene anti-bacterial compound solution disclosed by the invention, graphene is grafted with a surfactant through a nano-microcapsule technology, so that functional graphene is embedded in the surfactant, and cell structures of bacteria and mites are destroyed by a nano knife formed by utilizing graphene nano-microcapsules. In the graphene anti-bacterial compound solution disclosed by the invention, the graphene and the surfactant have a synergistic effect, and anti-bacterial and anti-mite functions are realized; compared with an anti-bacterial agent and an anti-mite agent existing in the market, the graphene antibacterial compound solution has the advantages that the graphene antibacterial compound solution is green, environmentally friendly and nontoxic.

The present invention provides a supermolecule hybrid hydrogel having excellent antibacterial property, a preparation method and applications thereof. The preparation method comprises that Fmoc-Glu-OMe adopted as a gelatin factor, silver nitrate and chitosan are subjected to co-assembly under an ultrasound effect to form a clarified and transparent supermolecule hybrid hydrogel, wherein the uniform size and monodispersed silver nanoparticles are subjected to in situ synthesis in the hydrogel, and the antibacterial property of the supermolecule hybrid hydrogel is substantially improved with the synergistic effect of the three components such as the Fmoc-Glu-OMe, the silver nanoparticles and the chitosan. According to the present invention, the supermolecule hybrid hydrogel has performances such as self-healing, injectability, capability of coating, and the like; the preparation method is simple, has the low cost, and can be commercialized; during the preparation process, any reducing agents and any dispersants are not added, and the silver nanoparticles are synthesized through the ultrasonic in situ synthesis, such that the green environment protection advantage is achieved; and various components of the supermolecule hybrid hydrogel provide the synergistic effect to make the antibacterial property of the composite material be significantly increased, such that the supermolecule hybrid hydrogel has wide application prospects in the field of the antibacterial material and the wound healing promotion.

The invention relates to a preparing method of a heterogeneous acellular dermal matrix substrate with good biocompatibility. The method concretely comprises the following steps of (1) split thickness skin graft preparation; (2) epidermis and dermis separation; (3) acellular processing; (4) cross linking; (5) virus inactivation; (6) refining; (7) Co-60 irradiation sterilization. The ecellular tissue engineering materials obtained by the method provided by the invention has the advantages that the toxicity is stable, and the cell compatibility is better.