16results about How to "Non-cytotoxic" patented technology

The invention provides a high-water-absorption sodium alginate hydrogel dressing which is composed of a solution A and a solution B. The solution A is a sodium alginate solution, and the solution B is a calcium ion solution which comprises poloxamer, zinc hyaluronate and carbomer 941. The mass concentration range of the sodium alginate solution is 1%-7%, and the concentration range of the calcium ion solution is 100-400 mM; the volume ratio of the solution A to the solution B is (1: 2)-(3: 1). The smooth and transparent moist dressing can be rapidly obtained, the dressing can be customized according to the shape and size of a wound, the moisture retention rate is 86% or above, the swelling rate is 4000% or above, wound seepage can be absorbed, the moist environment of the wound is kept, and therefore wound healing is promoted.

The invention discloses a medicine for treating osteoarthritis, and belongs to the field of medicine, the medicine comprises a reagent capable of stimulating chondrocyte development, and the medicine L-arginine can promote expression of chondrocyte anabolic factors and reduce expression of cartilage degradation markers, so that chondrocytes are protected to a certain extent; in addition, the medicine provided by the invention has an excellent cartilage repair function, can inhibit the progress of degenerative osteoarthritis, has good biocompatibility, and has no cytotoxicity to cartilage cells.

The invention discloses an application of combination of valnemulin and polymyxin in preparation of a bactericide. The valnemulin and the polymyxin cooperate to kill bacteria, the antibacterial activity of the polymyxin on gram-negative bacteria which are drug-resistant and sensitive to the polymyxin such as escherichia coli, klebsiella pneumoniae and acinetobacter pideri can be effectively recovered in vitro and in vivo, in addition, the valnemulin has no cytotoxicity to mammalian cells, and the antibacterial activity of the polymyxin on the gram-negative bacteria can be effectively recovered. The application of valnemulin effectively reduces the therapeutic dose of polymyxin, and reduces the side effect risk of polymyxin on mammals.

The invention discloses an induction type gelation antibacterial dressing, and belongs to the technical field of medical instruments. According to the induction type gelation antibacterial dressing, the hypochlorous acid and the white pine essential oil are used as antibacterial agents, and the hypochlorous acid and the white pine essential oil are easy to penetrate through cell walls and cell membranes of microorganisms and invade into the cells to carry out oxidation reaction by utilizing the neutral small molecule characteristics of the hypochlorous acid and the white pine essential oil, so that the microorganisms are killed; the gel further comprises a gel induction agent, namely boloxamer, sophorolipid, ethylenediamine tetraacetic acid and undecylenamide propyl betaine. By utilizing the characteristic that the boloxamer is sensitive to temperature and taking the boloxamer as a gelling agent of the dressing, the conversion between a gel state and a liquid state of the dressing is realized through temperature change, and necrotic tissues can be softened in 2-5 minutes, so that the residual dressing, the necrotic tissues and pollutants on a wound surface can be conveniently removed, and painless debridement is realized.

The present application relates to the technical field of biology, and particularly discloses a polypeptide, a recombinant humanized collagen of type XIX and application thereof. The amino acid sequence of the polypeptide comprises n repeating units; the amino acid sequence of the repeating unit is shown in SEQ ID NO. 2. The polypeptide has a collagen triple helix structure, has good cell adhesion activity, can perform the function of human collagen, has no immune rejection and allergic reaction, and can be used in various fields such as cartilage repair, biological dressing, tissue filling and the like.

Disclosed are ketone amide derivatives and applications thereof, and specifically disclosed are compounds of formula (IV) and pharmaceutically acceptable salts thereof.

The invention relates to a premixed calcium silicate / calcium formate self-curing root canal filling material as well as a preparation method and application thereof. The premixed root canal filling material which is short in curing time, high in mechanical strength and excellent in injectable performance is prepared by taking the calcium formate powder and the calcium silicate compound as main phases and adding at least one organic phase solvent which can be mixed and dissolved with water and at least one radiation-resistant material. The material keeps fluid under a closed condition, and is hydrated, solidified and hardened after being filled into teeth and contacted with physiological body fluid. The invention combines the advantages of calcium formate and calcium silicate materials, has the advantages of short curing time, good biological activity, good sealing property and antibacterial property, is convenient for clinical use, and can be used as a filling material in the dental restoration fields such as pulp capping, root canal therapy and the like.

This invention discloses an antimicrobial peptide, Cih1, and its applications. The amino acid sequence of antimicrobial peptide Cih1 is shown in SEQ ID NO.1. The antimicrobial peptide Cih1 provided by this invention is a cationic peptide, rich in cysteine ​​(-cys) residues, which can form an amphiphilic β-sheet secondary structure. This structure endows it with excellent antibacterial and antiviral activity, and can effectively inhibit the proliferation and invasion of pathogens in aquaculture water. Based on the broad-spectrum resistance of antimicrobial peptide Cih1 to pathogens, it can help aquatic animal hosts resist infection by various pathogens, and has broad application prospects in the field of anti-infective drug development for aquaculture.

The invention provides a preparation method of an adsorption material capable of efficiently adsorbing HIV viruses and the adsorption material, and belongs to the technical field of adsorption materials.The preparation method comprises the following steps that CA is added into a solution containing acetone and dimethyl sulfoxide under stirring, stirring continues to enable CA to be completely dissolved, and a CA solution is obtained; adding MIL-125 (Ti) into the CA solution, stirring, and further performing ultrasonic dispersion, so that the MIL-125 (Ti) is uniformly dispersed into the CA solution; water is used as a precipitation bath, the obtained solution is dropwise added into the precipitation bath, and the CA / MIL-125 (Ti) composite microspheres are obtained through phase inversion. The CA / MIL-125 (Ti) composite microspheres prepared by the invention have the advantages of multi-stage adsorption sites, excellent adsorption performance, good blood compatibility and high mechanical strength.

Disclosed are ketone amide derivatives and applications thereof, and specifically disclosed are compounds of formula (IV) and pharmaceutically acceptable salts thereof.

This invention discloses a method for preparing soluble regenerated silk fibroin lyophilized powder with controllable molecular weight, yielding a lyophilized powder that exists primarily in a SilkⅠ aggregated state and can be rapidly and completely dissolved. By optimizing the degumming, dissolution, dialysis purification, and lyophilization processes, combined with clarification filtration and low-temperature control, this invention overcomes the stability and quality problems of existing soluble regenerated silk fibroin preparation methods. By controlling key production process parameters, it is possible to prepare regenerated silk fibroin lyophilized powders with different molecular weight ranges for application in the preparation of biodegradable medical materials with varying properties.

The invention relates to the field of organic synthesis, in particular to a polyhydroxy benzophenone derivative and a preparation method thereof, the structural formula of the polyhydroxy benzophenone derivative is as shown in the following formula (1): # imgabs0 #, the # imgabs0 # has a diphenyl and polyhydroxy structure similar to flavonoid compounds and resveratrol, can provide reactive hydrogen to inactivate free radicals, has better scavenging capacity on. DPPH free radicals at low concentration, and can be used for preparing a novel compound. The structure of the hydroxyquinoline plays a positive role in improving the antioxidant activity, the synthesis mode is simple, no cytotoxicity exists basically at low concentration, and reference and help can be provided for research and development of novel cosmetics and various antioxidants.

The invention discloses a complex microbial inoculant as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The complex microbial inoculant disclosed by the invention is prepared by co-fermentation of lactobacillus plantarum CGMCC (China General Microbiological Culture Collection Center) NO.12849 and enterococcus faecium CGMCC The complex microbial inoculant has the characteristics of high antibacterial activity, safety, no toxicity, high stability and the like, and can be used for killing clinically common gram-negative pathogenic bacteria. By utilizing the high-density fermentation culture medium, the fermentation process and the preparation process provided by the invention, the complex microbial inoculant can be produced on a large scale and is low in cost, and the complex microbial inoculant is expected to replace antibiotics to become a safe, green and efficient ideal antibacterial agent and has a very wide application prospect.

The present application provides a kind of polypeptide sustained-release hydrogel coating for preventing catheter urinary tract infection and its preparation method, the coating includes the adhesive bottom layer attached to the surface of catheter substrate, and the polypeptide sustained-release hydrogel functional layer compounded outside the adhesive bottom layer;The functional layer includes hydrophilic hydrogel gel-forming matrix, targeted antibacterial polypeptide, polypeptide sustained-release carrier and biocompatible auxiliary agent.The present application solves the interface bonding problem of hydrophobic catheter substrate and hydrophilic hydrogel through the adhesive bottom layer, the coating has strong adhesion, is resistant to urine immersion and rubbing;Through polypeptide sustained-release carrier, antibacterial polypeptide is released for 30 days, and there is no risk of burst release;The targeted antibacterial polypeptide used is specific to urinary tract infection pathogenic bacteria, has no drug resistance induction risk, has excellent biocompatibility, can effectively prevent catheter-related urinary tract infection caused by short-term and long-term indwelling catheter, the preparation process is simple and controllable, is suitable for large-scale production, and can be widely applied to antibacterial modification of urinary intervention medical devices.

The present invention provides the use of phenylacetic acid compounds in the preparation of osteoclast differentiation inhibitors, and relates to the field of marine natural products. A phenylacetic acid compound is disclosed, and its structural formula is shown in formula (I), and it is named 4-butoxy-benzeneacetic acid. It has a significant inhibitory effect on LPS-induced NF-κB luciferase at a concentration of 10 μM, and can significantly inhibit the differentiation of RANKL-induced osteoclast precursor cells RAW264.7 cells and BMMs cells into osteoclasts at a concentration of 6 μM. It has a protective effect on bone destruction in mice with osteoporosis caused by ovariectomy by inhibiting osteoclasts in vivo. It can effectively replace existing bisphosphonates and denoximab, inhibit the bone resorption activity of osteoclasts, and is an ideal candidate compound for development into a new osteoclast differentiation inhibitor drug. #imgabs0#

The invention belongs to the technical field of anti-inflammatory pharmacy, and relates to a compound for inhibiting IL-1beta as well as a preparation method and application thereof, and the structural formula of the compound for inhibiting IL-1beta is shown as a formula I or a formula II. The compound for inhibiting the IL-1beta or the pharmaceutically acceptable salt thereof has the activity of inhibiting the IL-1beta, does not generate cytotoxicity, and can be used for preparing medicines for treating diseases caused by high expression of the IL-1beta; the invention develops a method for rapidly constructing a series of compounds for inhibiting IL-1beta from the raw materials which are simple and easy to obtain, and the anti-inflammatory potential of the Disprocochleroids A-C derivatives can be further explored. # imgabs0 #