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Bi-network high-intensity fibroin hydrogel and preparation method thereof

A silk protein, high-strength technology, used in medical science, prosthesis, tissue regeneration, etc., can solve the problems of limitation, surfactant cytotoxicity, lack of mechanical properties of hydrogels, etc., to achieve excellent performance and good repeatability , good biocompatibility and biodegradability

Inactive Publication Date: 2016-11-23
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the research on the preparation of high-strength silk protein hydrogel, it has been reported that the characteristics of surfactants are used to prepare high-strength silk protein hydrogel (silk protein / water: 15 / 85 (w / w)), and its compression mold However, the hydrogel prepared by silk protein at a low concentration does not have good mechanical properties, and the surfactant will have certain cytotoxicity; some studies have also reported the use of silk protein and cellulose derivatives preparation of high-strength composite hydrogel (CN 104845382 A), which also has certain restrictions on the concentration of silk protein

Method used

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  • Bi-network high-intensity fibroin hydrogel and preparation method thereof
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Examples

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Embodiment 1

[0032] Take 2 mL of mulberry silk protein solution with a mass fraction of 10 wt%, add 40 μL of 0.5 mg / mL horseradish peroxidase, mix evenly, add 40 μL of 0.05% hydrogen peroxide aqueous solution, and form a gel at 37 °C network; then soak it in 75% ethanol solution for 24 h to obtain a high-strength silk protein hydrogel with a compressive modulus of 1.37 MPa and a tensile modulus of 3.35 MPa. Tests show that it has stable performance and good cell compatibility under simulated in vivo environmental conditions.

Embodiment 2

[0034] Take 4 mL of tussah silk protein solution with a mass fraction of 2 wt%, add 80 μL of 1 mg / mL horseradish peroxidase, mix evenly, add 80 μL of 0.2% hydrogen peroxide aqueous solution, and form a gel at 40 °C network; then soak it in 70% isopropanol solution for 12 h to obtain a high-strength silk protein hydrogel with a compressive modulus of 0.28 MPa and a tensile modulus of 0.2 MPa. Tests show that it has stable performance and good cell compatibility under simulated in vivo environmental conditions.

Embodiment 3

[0036] Take 2 mL of tussah silk protein solution with a mass fraction of 20 wt%, add 40 μL of 0.1 mg / mL horseradish peroxidase, mix evenly, add 40 μL of 0.01% hydrogen peroxide aqueous solution, and form a gel at 25 °C network; then soak it in 0.5M sodium dodecyl sulfate solution for 48 h to obtain a silk protein hydrogel with good mechanical properties, with a compressive modulus of 3.2 MPa and a tensile modulus of 3.5 MPa. Tests show that it has stable performance and good cell compatibility under simulated in vivo environmental conditions.

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Abstract

The invention belongs to the technical field of natural polymer materials and particularly discloses bi-network high-intensity fibroin hydrogel and a preparation method thereof. Fibroin hydrogel subjected to chemical crosslinking (a first network) of horse radish peroxidase is soaked in a solution which can induce fibroin beta to fold and transform, physical crosslinking (a second network) is formed, and then the bi-network high-intensity fibroin hydrogel with the solid content being 2-20 wt% can be prepared. The hydrogel is good in elasticity, high in intensity and easy to regulate and has biocompatibility, the performance is stable under the condition of the simulated in-vivo environment, and the bi-network high-intensity fibroin hydrogel can be widely used for fields of drug sustained release carriers, cartilage tissue, ligament tissue and other biomedicine and regeneration medicine engineering.

Description

technical field [0001] The invention belongs to the technical field of natural macromolecular materials, and in particular relates to a double-network high-strength natural biological macromolecular hydrogel and a preparation method thereof. Background technique [0002] With the development of the field of tissue engineering, the demand for biomedical materials and their preparation technologies with excellent performance and suitable for use in a biologically friendly environment is increasing. Hydrogel is a cross-linked network rich in a large number of water molecules, has strong permeability, and is similar to extracellular matrix in structure and function, so hydrogel is not only an important class of cell scaffolds that can be used to rebuild tissue engineering It is also a kind of promising drug sustained-release carrier material. [0003] As a natural polymer material, silk protein has abundant sources, excellent mechanical properties, controllable degradation prop...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075A61L27/52A61L27/22C08L89/00
CPCC08J3/24A61L27/227A61L27/52A61L2430/40C08J3/075C08J2389/00C08L89/00
Inventor 邵正中苏帝翰宓瑞信陈新
Owner FUDAN UNIV
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