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Injectable articular cartilage tissue repair material and its preparation method

A technology for articular cartilage and tissue repair, applied in medical science, prosthesis, etc., can solve the problems of inconvenient application of seed cells and small amount of chondrocytes extracted at one time, and achieve good biological safety, no cytotoxicity, trauma small effect

Inactive Publication Date: 2012-06-13
成都普川生物医用材料股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If autologous chondrocytes are used as seed cells for cartilage tissue engineering scaffolds, since chondrocytes cannot be obtained immediately and the amount of one-time extraction is not large, it needs to be subcultured several times to obtain a sufficient amount of seed cells, so this kind of seed cells is clinically used. Very inconvenient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 uses 100 parts of type I collagen and 10 parts of oxidized hyaluronic acid; the degree of oxidation of oxidized hyaluronic acid is 20%, and the thickness of the composite material is 3 mm.

[0042] (1) In an ice-water bath, dissolve collagen in a hydrochloric acid solution with a concentration of 0.003M, stir slowly, prepare a collagen solution with a concentration of 9mg / ml, and place it in a refrigerator at a temperature of 4°C;

[0043] (2) Dissolving hyaluronic acid powder in ultrapure water, stirring to make it fully dissolve, and preparing a hyaluronic acid solution with a concentration of 10 mg / ml;

[0044] (3) Dissolving the sodium periodate powder in the dark in ultrapure water, stirring and preparing a sodium periodate solution with a concentration of 20mM;

[0045] (4) Add 5ml of the sodium periodate solution of step (3) to the hyaluronic acid solution of 20ml of step (2), stir at room temperature in the dark, react for 5h, dialyze the reaction solu...

Embodiment 2

[0053] Example 2 uses 100 parts of type I collagen and 20 parts of oxidized hyaluronic acid; the degree of oxidation of oxidized hyaluronic acid is 20%, and the thickness of the composite material is 3 mm.

[0054] (1) In an ice-water bath, dissolve collagen in a hydrochloric acid solution with a concentration of 0.003M, stir slowly, prepare a collagen solution with a concentration of 9mg / ml, and place it in a refrigerator at a temperature of 4°C;

[0055] (2) Dissolving hyaluronic acid powder in ultrapure water, stirring to make it fully dissolve, and preparing a hyaluronic acid solution with a concentration of 10 mg / ml;

[0056] (3) Dissolving the sodium periodate powder in the dark in ultrapure water, stirring and preparing a sodium periodate solution with a concentration of 20mM;

[0057] (4) Add 5ml of the sodium periodate solution of step (3) to the hyaluronic acid solution of 20ml of step (2), stir at room temperature in the dark, react for 5h, dialyze the reaction solu...

Embodiment 3

[0065] Example 3 uses 100 parts of type I collagen and 20 parts of oxidized hyaluronic acid; the degree of oxidation of oxidized hyaluronic acid is 40%, and the thickness of the composite material is 3 mm.

[0066] (1) In an ice-water bath, dissolve collagen in a hydrochloric acid solution with a concentration of 0.003M, stir slowly, prepare a collagen solution with a concentration of 9mg / ml, and place it in a refrigerator at a temperature of 4°C;

[0067] (2) Dissolving hyaluronic acid powder in ultrapure water, stirring to make it fully dissolve, and preparing a hyaluronic acid solution with a concentration of 10 mg / ml;

[0068] (3) Dissolving the sodium periodate powder in the dark in ultrapure water, stirring and preparing a sodium periodate solution with a concentration of 20mM;

[0069] (4) Add 10 ml of the sodium periodate solution of the step (3) to the hyaluronic acid solution of the 20 ml step (2), stir at room temperature in the dark, react for 8 hours, dialyze the ...

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Abstract

The invention discloses an injectable articular cartilage tissue repair material and is characterized in that starting materials of the composite material contain the following components of: by weight, 100 parts of I-type collagen and 10-50 parts of hyaluronic acid, wherein oxidisability of hyaluronic acid is 20-60%. A monocyte layer in bone marrow fluid in vivo is embedded in the repair material. The thickness of the composite material is 1-5mm. Hyaluronic acid is oxidized into oxidized hyaluronic acid by the use of sodium periodate. The collagen solution, 5*PBS and a buffer are mixed at the volume ratio of 7:2:1 and the pH is adjusted to neutral. After dissolving oxidized hyaluronic acid by the use of ultrapure water, the dissolved oxidized hyaluronic acid is added into the neutral collagen solution and the mixed solution is uniformly stirred and stands in a refrigerator of 4 DEG C. The separated and extracted monocyte layer in the bone marrow fluid by a density gradient centrifugation method is uniformly mixed with the above collagen-oxidized hyaluronic acid solution. The mixed liquor is putted into a constant temperature incubator of 37 DEG C for standing or injection to the organism articular cartilage defects so as to form the composite material.

Description

technical field [0001] The invention relates to an injectable articular cartilage tissue repair material and a preparation method thereof, belonging to the field of preparation of tissue repair materials. technical background [0002] Articular cartilage defect is a common disease, but there has been no very effective clinical treatment, and the content of cartilage tissue cells is low, unable to migrate, and lacks blood vessels, lymph and nerves, etc., and its self-repair ability is very limited. Applying tissue engineering to construct cartilage tissue engineering scaffolds to repair cartilage defects will be a very potential clinical treatment. [0003] Collagen is an important component of the extracellular matrix of animals. It has good biocompatibility, biodegradability and weak antigenicity, and has become one of the most widely used materials in the field of biomedicine. As a component of natural cartilage extracellular matrix, hyaluronic acid can specifically bind ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/24A61L27/20
Inventor 樊渝江岩晓丽郭立坤蒋波张兴栋
Owner 成都普川生物医用材料股份有限公司
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