Antibacterial supermolecule hybrid hydrogel, preparation method and applications thereof

A hydrogel and supramolecular technology, applied in medical science, absorbent pads, bandages, etc., can solve problems such as poor biocompatibility, complicated preparation process, and impact on antibacterial performance, and achieve easy injection, good biocompatibility, low cost effect

Active Publication Date: 2016-11-23
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, many documents and patents have reported the use of silver nanoparticle gel as an antibacterial agent, but most of them are unevenly dispersed, and the agglomeration phenomenon is serious, and the antibacterial performance is seriously affected (Chinese invention patent publication CN102218155A); In the case of particles, the chemical reagents with high toxicity are used, the cytotoxicity is high, and the biocompatibility is relatively poor (Chinese Invention Patent Publication CN101664563A); Invention patent publication CN102698313A)

Method used

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  • Antibacterial supermolecule hybrid hydrogel, preparation method and applications thereof
  • Antibacterial supermolecule hybrid hydrogel, preparation method and applications thereof
  • Antibacterial supermolecule hybrid hydrogel, preparation method and applications thereof

Examples

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Embodiment 1

[0037] Weigh 10.0 mg of Fmoc-Glu-OMe solid powder, dissolve it in 100 μL dimethyl sulfoxide, disperse ultrasonically for 1 min, and dissolve it completely to prepare a Fmoc-Glu-OMe stock solution with a concentration of 100 mg / mL; weigh 20.0 Add 1.0mL of ultrapure water to dissolve it in a brown light-proof centrifuge tube, take a little silver nitrate solution in a 15mL test tube that is protected from light with tin foil, add a certain amount of ultrapure water to dilute, and prepare The silver nitrate aqueous solution that becomes 2mmol / L is used as silver nitrate stock solution; Take the chitosan of 80mg, add the ultrapure water of 8.0mL to dissolve it, then add the glacial acetic acid that volume ratio is 1% to promote the dissolving of chitosan , dubbed 10mg / mL chitosan stock solution.

[0038] Take 20 μL of Fmoc-Glu-OMe stock solution and add it to a 5.0 mL screw bottle that has been washed, dried and protected from light, quickly add 490 μL of silver nitrate stock solu...

Embodiment 2

[0043] Take 20 μL of the Fmoc-Glu-OMe stock solution in the above Example 1, add it to a 5.0 mL screw bottle that has been washed, dried and protected from light, and quickly add 490 μL of the 4 mmol / L silver nitrate stock solution prepared in the same way as in Example 1. Solution, shake up rapidly, then add 490 μ L of the chitosan stock solution of 20 mg / mL identical to the preparation method of Example 1, shake up rapidly. A clear and transparent supramolecular hybrid hydrogel can be formed after putting the screw bottle into an ultrasonic cleaner for one hour.

[0044] Mouse experiment steps: the mouse model was selected to study the effect of the supramolecular hybrid hydrogel of the present invention on wound healing. The whole experiment was completed in Xiangya Medical College. The 6 mice used in the experiment were male, SD rats with a mass of about 260g, to evaluate the therapeutic effect of the present invention on wounds. First weigh the mouse, anesthetize the mou...

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Abstract

The present invention provides a supermolecule hybrid hydrogel having excellent antibacterial property, a preparation method and applications thereof. The preparation method comprises that Fmoc-Glu-OMe adopted as a gelatin factor, silver nitrate and chitosan are subjected to co-assembly under an ultrasound effect to form a clarified and transparent supermolecule hybrid hydrogel, wherein the uniform size and monodispersed silver nanoparticles are subjected to in situ synthesis in the hydrogel, and the antibacterial property of the supermolecule hybrid hydrogel is substantially improved with the synergistic effect of the three components such as the Fmoc-Glu-OMe, the silver nanoparticles and the chitosan. According to the present invention, the supermolecule hybrid hydrogel has performances such as self-healing, injectability, capability of coating, and the like; the preparation method is simple, has the low cost, and can be commercialized; during the preparation process, any reducing agents and any dispersants are not added, and the silver nanoparticles are synthesized through the ultrasonic in situ synthesis, such that the green environment protection advantage is achieved; and various components of the supermolecule hybrid hydrogel provide the synergistic effect to make the antibacterial property of the composite material be significantly increased, such that the supermolecule hybrid hydrogel has wide application prospects in the field of the antibacterial material and the wound healing promotion.

Description

technical field [0001] The present invention relates to the field of supramolecular hydrogels, in particular to using Fmoc-Glu-OMe as a hydrogel factor, ultrasonically synthesizing silver nanoparticles in situ, adding chitosan, and co-assembling with amino acids to form an antibacterial supramolecular hybrid Preparation method and application of composite hydrogel. Background technique [0002] Despite the rapid development of medical technology, millions of people around the world suffer from infectious diseases caused by bacteria every year. In addition, bacterial resistance to traditional antimicrobial agents is increasing, and the problem of infectious diseases caused by bacteria remains an important health problem to be solved urgently. Therefore, it is becoming more and more important to design and develop new antibacterial agents, especially in the field of biomedical chemistry (Proc. Natl. Acad. Sci. U.S.A., 2006, 103, 15997-16002). Antimicrobial agent is a materia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L5/08C08K3/08A61L15/28A61L15/20A61L15/18A61L15/44A61L15/42
Inventor 张翼张珍珠孙志方袁孟颖
Owner CENT SOUTH UNIV
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