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A bioink for 3D printing

A bio-ink and 3D printing technology, applied in the field of ink, can solve the problems of difficult to balance cell compatibility, bioactivity and mechanical properties, scaffold mechanical properties, poor simulation and stability, and inability to mix and print, etc., to achieve excellent biological Compatibility, good structural stability, reduced shrinkage or swelling effects

Active Publication Date: 2018-07-31
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although 3D printing technology has attracted enough attention in the fields of biomedicine, such as disease models, drug sustained release, tissue engineering and regenerative medicine, so far, the use of 3D printing technology to manufacture organs or tissues that are truly suitable for implantation in the body still faces great challenges. challenges, of which developing biomaterials suitable for 3D printing is key
Most of the above-mentioned synthetic materials are 3D printed with organic solvents or high temperature. The printed scaffold structure is stable, but the accuracy is limited, and it cannot be mixed with cells for printing.
Water-soluble natural polymer materials can be printed simultaneously with cells and maintain their activity, but the mechanical properties, simulation and stability of the scaffold are poor
Existing materials are difficult to balance cell compatibility, bioactivity and mechanical properties whether used alone or in combination

Method used

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  • A bioink for 3D printing
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A bio-ink for 3D printing is composed of polyether ester, hyaluronic acid, bioactive components, cross-linking initiators and solvents. The bioactive components of this embodiment use acellular matrix, and its preparation method is as follows:

[0027] (1) Use water-soluble PEG to initiate TMC polymerization to obtain PTMC-PEG-PTMC triblock polyether ester copolymer, and modify the end group with double bonds to obtain water-soluble polyether ester PFMC;

[0028] (2) modifying the hydroxyl group of hyaluronic acid with a double bond to obtain a water-soluble natural polymer with a cross-linking function;

[0029] (3) Decellularize the nerve tissue by a decellularization method to obtain decellularized nerve tissue, and pulverize the decellularized nerve tissue to obtain a water-soluble decellularized nerve matrix material;

[0030] (4) Take 0.5% decellularized matrix powder by mass fraction and digest it with pepsin hydrochloric acid solution (i.e. digestive juice) to b...

Embodiment 2

[0033]A bio-ink for 3D printing is composed of polyether ester, hyaluronic acid, bioactive components, cross-linking initiators and solvents. The bioactive components of this embodiment use acellular matrix, and its preparation method is as follows:

[0034] (1) Use water-soluble F127 to initiate TMC polymerization to obtain PTMC-F127-PTMC three-block polyether ester copolymer, and modify the end group with double bonds to obtain water-soluble polyether ester PFMC;

[0035] (2) modifying the hydroxyl group of hyaluronic acid with a double bond to obtain a water-soluble natural polymer with a cross-linking function;

[0036] (3) Decellularize the tendon tissue by a decellularization method to obtain decellularized tendon tissue, and pulverize the decellularized tendon tissue to obtain a water-soluble decellularized nerve matrix material (biologically active component);

[0037] (4) Take 1% acellular matrix powder by mass fraction and digest it with pepsin hydrochloric acid solu...

Embodiment 3

[0042] A bio-ink for 3D printing is composed of polyether ester, hyaluronic acid, bioactive components, cross-linking initiators and deionized water. The bioactive components of this embodiment use short peptides, and the preparation method is as follows:

[0043] (1) Use water-soluble F127 to initiate TMC polymerization to obtain PTMC-F127-PTMC three-block polyether ester copolymer, and modify the end group with double bonds to obtain water-soluble polyether ester PFMC;

[0044] (2) modifying the hydroxyl group of hyaluronic acid with a double bond to obtain a water-soluble natural polymer with a cross-linking function;

[0045] (3) According to the mass fraction, 1% RADARADARADARADA-RGD short peptide, 1% hyaluronic acid, 8% water-soluble polyether ester PEMC, and 0.05% photoinitiator I2959 were dissolved in 100% deionized water to prepare a bioink.

[0046] Take 20ml of the bio-ink and 1ml 5×10 5 After the neural stem cells in cells / ml are mixed, they can be photocrosslinke...

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PUM

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Abstract

The invention discloses biological ink for 3D printing. The biological ink for 3D printing comprises water-soluble synthetic polymer with the cross-linking function, water-soluble natural polymer with the cross-linking function, bioactive components capable of spontaneously forming special ultrastructures, cross-linking initiator and solvents and further comprises bioactive components. The biological ink for 3D printing overcomes the defects that traditional 3d printing ink is single in component structure, does not have good biological activity and needs to utilize organic solvents, and it is difficult to take cell compatibility, biological activity and mechanical properties into consideration, the biological ink which has biological activity and the fast curing function at the same time and can form a certain microstructure is obtained, and hydrogel obtained through curing of the ink has controllable mechanical properties, good structural stability and a good fidelity effect.

Description

technical field [0001] The invention relates to an ink, in particular to a bio-ink used for 3D bio-printing to manufacture bio-scaffold materials such as bio-tissues and organs. Background technique [0002] 3D printing technology (also known as 3D rapid prototyping technology or additive manufacturing technology) is an emerging manufacturing technology that has emerged in recent years. This technology is mainly under computer control, according to the computer-aided design (CAD) model or computer tomography of the printed object Scanning (CT) is a digital molding technology that quickly manufactures 3D objects of a specified shape through the accurate 3D accumulation of materials and the converted model after the data is obtained. With the continuous advancement of 3D printing technology, its application fields include daily necessities, mechanical equipment, biomedicine, etc. Although 3D printing technology has attracted enough attention in the fields of biomedicine, such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D11/104C09D11/04C09D11/14C09D11/03
Inventor 全大萍刘小林刘晟王涛杨伟红刘江辉杨习锋曾晨光朱庆棠郑灿镔
Owner SUN YAT SEN UNIV
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