Kit for inducing differentiation from bone mesenchymal stem cells to osteoblasts, application of kit and method for inducing cell differentiation

Abstract

The invention provides a kit and method for inducing differentiation from bone mesenchymal stem cells to osteoblasts. The kit comprises an osteoblast inducing culture solution and an osteoblast identification solution, wherein the inducing culture solution comprises a cell culture solution A, a cell culture solution B, a cell culture solution C, a cell culture solution D and a cell culture solution E; the osteoblast identification solution comprises a cell immobilizing solution, coloring agents and a detergent; the cell culture solution A is an alpha-minimum essential medium (MEM) liquid culture medium; the cell culture solution B is fetal bovine serum; the cell culture solution C is a dexamethasone solution; the cell culture solution D is a beta-sodium glycerophosphate solution; the cell culture solution E is a vitamin C solution; the cell immobilizing solution is 4% paraformaldehyde; the coloring agents include sodium thiosulfate, silver nitrate and neutral red; and the detergent is double distilled water.

Description

technical field [0001] The present invention relates to a kit for inducing cell differentiation, its application and a method for inducing cell differentiation, in particular, to a kit for inducing differentiation of bone marrow mesenchymal stem cells into osteoblasts and its application and inducing bone marrow mesenchymal stem cells A method for differentiation of stem cells into osteoblasts. Background technique [0002] Bone marrow mesenchymal stem cells are a type of adult stem cells with multi-directional differentiation potential, which can be differentiated into various tissue cells such as osteoblasts, chondrocytes, osteoblasts, nerve cells, and hepatocytes. Gene therapy, cytokine replacement therapy, etc. Bone marrow mesenchymal stem cells have become an ideal source of seed cells for bone tissue engineering due to their incomparable advantages over other stem cells. [0003] The main reason for nonunion or delayed union after fracture is the lack of sufficient n...

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Problems solved by technology

[0004] In previous studies on the induction of bone marrow mesenchymal stem cells into osteoblasts, the inducer included newborn calf serum, penicillin, streptomycin, glutamine, dexamethasone, β-sodium glycerophosphate, vitamin C, low glucose Various materials such as DMEM not only increase the complexity of the operation, but also easily interfere with bone marrow mesenchymal stem cells and affect the transformation of bone marrow mesenchymal stem cells into osteoblasts

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