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Method for extracting and purifying neutral pseudo-ginseng polysaccharide, research and application for pharmacological activity for promoting cell proliferation

A technology of notoginseng polysaccharide and purification method, which is applied in the field of medicine, and can solve the problems that are not involved in the application and have not been chromatographically purified.

Active Publication Date: 2017-10-24
云南多糖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this invention is only a preliminary extraction, without chromatographic purification, and does not involve specific applications.

Method used

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  • Method for extracting and purifying neutral pseudo-ginseng polysaccharide, research and application for pharmacological activity for promoting cell proliferation
  • Method for extracting and purifying neutral pseudo-ginseng polysaccharide, research and application for pharmacological activity for promoting cell proliferation
  • Method for extracting and purifying neutral pseudo-ginseng polysaccharide, research and application for pharmacological activity for promoting cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A method for extracting and purifying neutral notoginseng polysaccharide, specifically comprising the following steps:

[0065] (1) Extraction of Panax notoginseng crude polysaccharide: Weigh 1 kg of the waste residue extracted from Panax notoginseng saponins, add 10 times the amount of water for the first time, cook in a boiling water bath for 8 hours, collect the filtrate, add 5 times the amount of water for the second time, and put it in a boiling water bath 8h. The two filtrates were combined, centrifuged at 4500rpm for 5min, and the supernatant was taken. Spin and concentrate at 80°C to 1 / 8 of the original volume, let it stand for 24 hours, centrifuge, discard the precipitate, slowly add three times absolute ethanol, stir while adding, place at 4°C for 12 hours, and filter to obtain the precipitate. Wash the precipitate several times with 75% ethanol, evaporate the ethanol to dryness, dissolve it with an appropriate amount of water, centrifuge, collect the superna...

Embodiment 2 10

[0087] Example 2 Effect of Panax notoginseng polysaccharide on the proliferation of human periodontal ligament stem cells, mouse osteoblast MC3T3-E1 and human skin epidermal cells in vitro

[0088] 1. MTT method to determine the effect of Panax notoginseng polysaccharide on the proliferation of human periodontal ligament stem cells in vitro

[0089] Cells were cultured using conventional cell culture methods. Human periodontal ligament stem cells in the logarithmic growth phase of 7 passages were taken, and the cell concentration was adjusted after counting. They were seeded in a 96-well plate, 100 cells / well, 90 μl per well, and cultured in a CO2 incubator for 12 hours. After adding PNPS I, PNPSII, and PNPS respectively 10μl each of III, PNPS IV and Panax notoginseng crude polysaccharide solution, so that the final concentration on the cells is 6.72mg / ml, 4.48mg / ml, 2.24mg / ml, 1.12mg / ml, 560ug / ml, 280ug / ml, 140ug / ml, 70ug / ml, 6 parallel wells were set up for each concentrat...

Embodiment 3

[0113] The notoginseng polysaccharide toothpaste was prepared by using the notoginseng neutral polysaccharide PNPS I with the highest content and the most significant cell proliferation-promoting activity as raw material.

[0114] This example discloses a toothpaste containing the notoginseng neutral polysaccharide PNPS I and its preparation method and application. The notoginseng polysaccharide toothpaste includes notoginseng neutral polysaccharide PNPS I and friction agent, wetting agent, adhesive, foaming agent, essence, sodium saccharin, sodium benzoate, food coloring, deionized water. The active ingredient in the toothpaste is 0.1-2.0% by weight of Panax notoginseng neutral polysaccharide PNPS I; the friction agent is calcium hydrogen phosphate dihydrate, silicon dioxide, calcium carbonate, hydroxide One or more of aluminum; the wetting agent is one or more of glycerin, sorbitol, and polyethylene glycol accounting for 2 to 55% by weight of the toothpaste; the binder is 0....

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Abstract

The invention relates to a method for extracting and purifying neutral pseudo-ginseng polysaccharide, a research and an application for pharmacological activity for promoting cell proliferation. The pseudo-ginseng waste residue of the notoginsenoside extracted in the production process is taken as a raw material, a water extract and alcohol precipitation method is adopted for acquiring crude pseudo-ginseng polysaccharide and the DEAE Sepharose Fast Flow anion exchange chromatography is adopted for purifying the crude pseudo-ginseng polysaccharide so as to acquire five components: neutral pseudo-ginseng polysaccharide PNPS I and acidic pseudo-ginseng polysaccharide PNPS II, PNPS III, PNPS IV and PNPS V five samples. The research on the influence of the pseudo-ginseng polysaccharide on human periodontal ligament stem cell, mice osteoblast and human skin epidermis cell in vitro proliferation proves that the neutral pseudo-ginseng polysaccharide PNPS I is effective in boosting the periodontal ligament stem cell, mice osteoblast and human skin epidermis cell in vitro proliferation and the neutral pseudo-ginseng polysaccharide PNPS I can be used as an important drug intermediate for developing a new drug and a natural raw material for a functional healthcare food and also can be used as an active raw material of the household chemicals.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a method for extracting and purifying neutral notoginseng polysaccharides and research and application of pharmacological activity for promoting cell proliferation. Background technique [0002] Polysaccharides widely exist in animals, plants and microorganisms. As one of the components of living matter, it is widely involved in the regulation of various life phenomena and physiological processes of cells, such as the transmission and perception of information between immune cells, cell transformation, division and regeneration and other activities. Since the 1980s, scientists have had a keen interest in the study of polysaccharides from traditional Chinese medicines. There are more than 100 kinds of polysaccharides reported so far, including ginseng polysaccharide, lentinan, ganoderma lucidum polysaccharide, astragalus polysaccharide, poria cocos polysaccharide, cordyceps polysaccharide, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C11D9/02C11D9/38C11D9/26C11D9/50A61K8/73A61K8/02A61K31/715A61Q19/00A61Q11/00A61Q19/10A61P1/02A61P7/04A61P17/00A23L33/125
CPCA23L33/125A23V2002/00A61K8/0212A61K8/73A61K31/715A61Q11/00A61Q19/00A61Q19/10C08B37/0003C11D3/48C11D9/005C11D9/02C11D9/262C11D9/38A23V2200/318A23V2200/312
Inventor 陈彤
Owner 云南多糖生物科技有限公司
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