Aggregation-induced emission nanoparticle and preparation method and application thereof

A technology of aggregation-induced luminescence and nano-particles, which is applied in the field of medical materials, can solve the problems of interfering with the normal physiological functions of cells, the toxicity of quantum dots with heavy metals, and the reduction of fluorescence intensity, so as to achieve cell survival and differentiation ability without interference and good tracer effect, the effect of high luminous efficiency

Active Publication Date: 2016-11-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fluorescent protein and luciferase require gene transfection, which may interfere with the normal physiological functions of cells; quantum dots are toxic to heavy metals, and their fluorescence intensity decreases

Method used

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  • Aggregation-induced emission nanoparticle and preparation method and application thereof
  • Aggregation-induced emission nanoparticle and preparation method and application thereof
  • Aggregation-induced emission nanoparticle and preparation method and application thereof

Examples

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[0038] Example 1

[0039] A method for preparing aggregation-induced luminescent nanoparticles includes the following steps:

[0040] (1) Distearoylphosphatidylethanolamine-polyethylene glycol-2000 (1mg), distearoylphosphatidylethanolamine-polyethylene glycol-2000-maleic anhydride (1mg), compound shown in formula I (R 1 -R 9 All hydrogen) (1 mg) was dissolved in 1 mL of tetrahydrofuran, and 9 mL of distilled water was added under ultrasound (80% output, SCIENTZ-II D ultrasonic instrument), and the ultrasound was continued for 2 minutes to prepare AIE nanoparticles modified with maleic anhydride.

[0041] (2) The tetrahydrofuran is subsequently removed by volatilization, and further filtered through a 0.2 μm filter to remove precipitates and large particulate matter. The cell penetrating polypeptide Tat (RKKRRQRRRC) (SEQ ID No. 1) was added to the filtrate, and after reacting overnight at room temperature, the excess polypeptide was removed by dialysis to prepare AIE nanoparticles th...

Example Embodiment

[0043] Example 2

[0044] A method for preparing aggregation-induced luminescent nanoparticles includes the following steps:

[0045] (1) Distearoylphosphatidylethanolamine-polyethylene glycol-2000 (1mg), distearoylphosphatidylethanolamine-polyethylene glycol-2000-carboxyl (1mg), the compound shown in formula I (R 3 It is tert-butyl and other substituents are hydrogen) (1mg) dissolved in 1mL of tetrahydrofuran, added to 9mL of distilled water under ultrasound (80% output, SCIENTZ-II D sonicator), and directly prepared surface modified Arima by nanoprecipitation method AIE nanoparticles made of acid anhydride.

[0046] (2) The tetrahydrofuran is subsequently removed by volatilization, and further filtered through a 0.2 μm filter to remove precipitates and large particulate matter. Add cell penetrating polypeptide Tat (RKKRRQRRRC) (SEQ ID No.1), 69μg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 78μg N-hydroxyl to the filtrate After reacting overnight at room temper...

Example Embodiment

[0047] Example 3

[0048] A method for preparing aggregation-induced luminescent nanoparticles includes the following steps:

[0049] (1) Distearoylphosphatidylethanolamine-polyethylene glycol-2000 (1mg), distearoylphosphatidylethanolamine-polyethylene glycol-2000-amino (1mg), the compound shown in formula I (R 3 It is a carboxyl group and other substituents are hydrogen) (1mg) dissolved in 1mL of tetrahydrofuran, added to 9mL of distilled water under ultrasound (80% output, SCIENTZ-II D sonicator), and directly prepared surface-modified maleic anhydride by nanoprecipitation AIE nanoparticles.

[0050] (2) The tetrahydrofuran is subsequently removed by volatilization, and further filtered through a 0.2 μm filter to remove precipitates and large particulate matter. Add cell penetrating polypeptide Tat (RKKRRQRRRC) (SEQ ID No.1), 69μg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 78μg N-hydroxyl to the filtrate After reacting overnight at room temperature, sulfosucc...

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Abstract

The invention discloses an aggregation-induced emission nanoparticle and preparation method and application thereof. The preparation method comprises the following steps: dissolving an aggregation-induced emission compound shown in a formula I and an amphiphilic polymer in a water-soluble organic solvent, then adding the mixture to water, and conducting ultrasonic reaction; removing organic solvents by volatilization to form nanoparticles loaded with the aggregation-induced emission compound; adding cell penetrating peptides in aqueous solution of the nanoparticle, reacting to form aggregation-induced emission nanoparticles capable of efficiently staining cells. The fluorescent particles can be used for tracing long-term differentiation of stem cells, especially the tracing of mouse bone marrow mesenchymal stem cells in the process of osteogenic differentiation. Compared with the commercial tracer reagent in the prior art, which can only trace 6 generations of cell proliferation, the nanoparticle has a better tracing effect, which can trace to more than 12 generations of cell proliferation. The aggregation-induced emission nanoparticle has the advantages of high cell staining efficiency, long-term tracing ability, high light stability and low cell toxicity.

Description

technical field [0001] The invention belongs to the field of medical materials, and in particular relates to an aggregation-induced luminescent nanoparticle and its preparation method and its application in stem cell tracing, especially its ability in tracing the differentiation process of mouse bone marrow mesenchymal stem cells to osteoblasts use. Background technique [0002] Bone injuries caused by trauma or disease are very common clinically. Traditional clinical treatment is mainly to achieve repair through autologous bone grafting. However, there are many defects in bone grafting, such as limited bone source, long operation time, and comprehensive complications after operation. [0003] In recent years, the application of stem cell therapy in bone repair has attracted increasing attention. For example, bone marrow mesenchymal stem cells are rich in sources, have strong differentiation and proliferation ability, can be cultured, expanded and differentiated in large ...

Claims

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Application Information

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IPC IPC(8): C09K11/07A61K49/00G01N21/64
CPCA61K49/0021C09K11/07G01N21/6486
Inventor 唐本忠高蒙赵祖金任力王迎军陈军建
Owner SOUTH CHINA UNIV OF TECH
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