Bone defect repair material based on modified perovskite quantum dots/amino carbon quantum dots and preparation method thereof

Abstract

The invention discloses a bone defect repair material based on modified perovskite quantum dots and a preparation method of the bone defect repair material. The bone defect repair material comprises the following raw materials in percentage by mass: 20-30% of hydroxyapatite, 10-20% of octacalcium phosphate, 0.5-2% of bone morphogenetic protein, 1-5% of collagen, 0.5-2% of chondroitin sulfate, 3-5% of carboxymethyl chitosan and the balance of a modified perovskite quantum dot solution. The modified perovskite quantum dot / amino carbon quantum dot solution is prepared from the following reaction raw materials in parts by mass: 0.5 to 1.5 parts of perovskite quantum dots, 8 to 16 parts of dipalmitoyl phosphatidyl ethanolamine, 3 to 7 parts of acidic amino acid, 1 to 3 parts of reduced glutathione, 0.5 to 1 part of EDC.HCl, 20 to 30 parts of chloroform and 100 parts of deionized water. Perovskite quantum dots and amino carbon quantum dots are introduced into the bone defect repair material for the first time, stem cell transfection is mediated by quantum dot and stem cell behavior changes are monitored by utilizing a fluorescence microscopy imaging technology; meanwhile, the stem cells are induced to differentiate towards osteoblasts and chondroblasts, and proliferation and regeneration of the osteoblasts are stimulated.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a bone defect repair material based on modified perovskite quantum dots / aminocarbon quantum dots and a preparation method thereof. Background technique [0002] A bone defect is a bone deficiency caused during a pathological process or during surgery, which disrupts the structural integrity of the bone. Clinically, trauma, tumors, osteomyelitis, various congenital diseases, and surgical operations may all cause bone defect symptoms. Due to the existence of bone defects, bone nonunion, delayed union or nonunion, and local dysfunction are often caused. At present, the methods for treating bone defects include autologous bone and allogeneic bone transplantation, tissue engineering technology, gene therapy rejection method, and physical factor therapy. However, autologous bone transplantation will cause secondary trauma, and allogeneic bone transplantation may cause immune reject...

AI Technical Summary

Problems solved by technology

However, due to its strong ionicity, high surface energy and metastable structure, perovskite quantum dots are highly sensitive to the environment, and are easy to cause phase transition, agglomeration or even degradation under the action of polar solvents, water and oxygen, thereby causing fluorescence quenching. off, especially with Sn 2+ As a B-site element, it is easily oxidized to Sn in humid air 4+ , exacerbating the main defects of halogen vacancies and interstitial metals, further accelerating the collapse of the perovskite structure

Due to the poor stability of perovskite quantum dots, its practical application is greatly limited

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