Preparation method of epicardial cells from stem cells

A technology of epicardial and pluripotent stem cells, applied in biochemical equipment and methods, embryonic cells, animal cells, etc. Complicated use and operation

Inactive Publication Date: 2017-05-10
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These two methods are costly and complicated to operate, and the animal-derived components contained in the differentiation medium are not conducive to the clinical use and research of the differentiated epicardial cells
These shortcomings hinder the large-scale study and use of epicardial cells

Method used

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  • Preparation method of epicardial cells from stem cells
  • Preparation method of epicardial cells from stem cells
  • Preparation method of epicardial cells from stem cells

Examples

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Embodiment Construction

[0035] The following examples are only used to further illustrate the content of the present invention, and are not intended to limit the present invention in any respect. Those skilled in the art will appreciate that modifications may be made to the detailed description described below without departing from the scope, spirit and spirit of the invention as claimed in the appended claims.

[0036] In all the following examples, unless otherwise stated, methods, instruments, reagents and experimental schemes commonly used by those skilled in the art are used. The basal medium for differentiation was RPMI 1640 (Gibco), and the differentiation supplement was S12 (patent pending) developed by our laboratory or commercial supplement B27 (Gibco). In all the following examples, unless otherwise stated, S12 is used as the differentiation supplement.

[0037] The human pluripotent stem cells used in the present invention are commercially used human embryonic stem cell lines from cell ...

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Abstract

The invention provides a preparation method of epicardial cells from multipotent stem cells. The method comprises the following steps: (1) by activating WNT signal pathways, inducing the multipotent stem cells to be differentiated into mesodermal cells; 2) by inhibiting the WNT signal pathways, converting the mesodermal cells obtained in the step 1) into myocardial precursor cells; 3) by activating retinoic acid signal pathways and the WNT signal pathways, converting the myocardial precursor cells obtained in the step 2) into anterior epicardial cells; and 4) preparing the epicardial cells from the anterior epicardial cells obtained in the step 3). The invention further relates to a method for preparing vascular smooth muscle cells and cardiac fibroblasts from the multipotent stem cells, the epicardial cells, the vascular smooth muscle cells and the cardiac fibroblasts prepared with the method, and application of the epicardial cells, the vascular smooth muscle cells and the cardiac fibroblasts.

Description

technical field [0001] The present application relates to a method for preparing epicardial cells from pluripotent stem cells, and also relates to a method for preparing vascular smooth muscle cells and cardiac fibroblasts from pluripotent stem cells, and epicardial cells, vascular smooth muscle cells and cardiac fibroblasts prepared by the above method Fibroblasts and their uses such as epicardial cells for cardiac injury repair and screening of new therapies for cardiac injury, and redifferentiation into vascular smooth muscle cells for disease and drug screening models, vascular tissue engineering. Background technique [0002] Epicardial lineage cells are required during embryonic heart development. Pre-Epicardial Cell Precursors: Pro-epicardial cells, derived from visceral mesoderm, develop in the inflow tract of the heart tube immediately posterior to the atrium and sinus venosus. The precise source of pre-epicardial cells is not well understood, but genetic lineage t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0656C12N5/0657C12N5/0661C12N2501/115C12N2501/15C12N2501/385C12N2501/415C12N2506/02C12N2506/45
Inventor 马跃赵建民
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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