20 results about "Liver perfusion" patented technology

The present invention provides a liver hypothermic machine perfusion preservation device and a method. The device includes a perfusion loop and a controller, wherein the perfusion loop includes a main pump, a main reservoir, an auxiliary pump and an auxiliary reservoir. The main pump is connected to an inlet of the main reservoir and configured to pump a perfusate from an organ cassette into the main reservoir; an outlet of the main reservoir is connected to a portal vein and a hepatic artery of a liver, and the main reservoir is configured to supply the perfusate to the portal vein and the hepatic artery; and the auxiliary reservoir and the auxiliary pump provide an additional hepatic artery perfusion flow path. According to the present invention, for a poor internal environment of the hepatic artery, the main reservoir and the auxiliary reservoir cooperate with each other to carry out perfusion, so that the perfusion efficiency and effect of the hepatic artery can be improved. The device according to the present invention also has a molecular-sieve oxygen generation function, and a free adjustment range of oxygen concentration from 21% to 90% can be achieved by means of combined control of a molecular-sieve oxygen generation channel and an air channel, thus providing a more flexible solution for the perfusion.

Methods are provided for effectively distinguishing the abilities of a human liver and an extracorporeal liver in an extracorporeal liver perfusion system to extract cholate and, thus, measure relative liver function.

The invention belongs to the technical field of in-vitro organ perfusion, and provides a sequential liver perfusion machine integrating acquisition, storage and transplantation. The device comprises an organ containing module which is provided with an organ containing basin; the in-vitro organ perfusion module is used for establishing perfusate circulation for the in-vitro organs placed in the organ accommodating basin; the in-vitro lung simulation module is used for inputting gas components into perfusate; the temperature adjusting module is used for adjusting the temperature of the perfusate; a perfusate exchange module; and the NRP module is used for injecting blood into the abdominal aorta of the donor and collecting blood flowing out of the inferior vena cava. Compared with the prior art, a clinical transplantation scheme is improved; a UW-MP, oxygen-containing carrier colloidal fluid and blood continuous exchange scheme is provided, and the organ quality is effectively improved; the convenience of preparation and use of the machine is improved, and the cost of consumables is reduced; the blood vessel tension change can be continuously and quickly coped with; in conclusion, the safety and stability of the perfusion system are obviously improved, and the mental pressure of medical staff is reduced.

The utility model relates to the field of perfusion devices, and discloses a mouse liver perfusion temperature and speed control device which comprises a perfusion table, a temperature control mechanism and a speed control mechanism. The temperature control mechanism comprises a thermostatic bath formed in the top of the filling table, a heating assembly fixedly installed on the surface of the inner side wall of the thermostatic bath, a temperature sensor fixedly installed on the surface of the inner bottom wall of the thermostatic bath and a liquid storage assembly movably placed in the thermostatic bath. The speed control mechanism comprises a base and a speed control assembly arranged on the base, one end of a liquid guide pipe is connected with the liquid storage assembly, and the other end of the liquid guide pipe is wound around the speed control assembly and then connected with an infusion needle. According to the mouse liver perfusion temperature and speed control device, the independent temperature control mechanism and speed control mechanism are designed, and the accuracy and flexibility of experimental operation are effectively enhanced through the design characteristics.

This invention belongs to the field of biomedical technology and provides a high-throughput drug screening system and its usage method for enriching circulating tumor cells (CTCs) in liver cancer. It includes an information processing and control unit; a diseased liver perfusion device comprising an organ chamber containing injection fluid at the bottom, an arterial supply line, an arterial end sensor, an arterial end clamp valve, at least two membrane lungs, a portal vein supply line, a portal vein end clamp valve, and a portal vein end sensor; a CTC enrichment device, which uses erythrocyte lysis to remove erythrocytes and immunomagnetic bead labeling to remove leukocytes, enabling the processing of large volumes of perfusion fluid in a single step to obtain enriched circulating tumor cells; a high-throughput drug screen; and phototubes, arterial end sensors, and portal vein end sensors, each electrically connected to the information processing and control unit. Compared with existing technologies, the high-throughput drug screening system and its usage method of this invention have the advantages of establishing a room-temperature mechanically perfused ex vivo liver cancer model, realizing the processing of large volumes of liquid specimens, and providing automated high-throughput drug screening functionality.

The present invention relates to the field of image processing, specifically to a liver perfusion control method and system based on image recognition. The method identifies thermal imaging images of an isolated liver during perfusion, obtains temperature data, and uses this data to calibrate abnormal activity areas of the isolated liver; identifies the changing trends of abnormal activity areas of the hepatic artery and portal vein, and estimates the circulatory degradation time nodes of the hepatic artery and portal vein; obtains metabolic differences based on metabolic data associated with the circulatory degradation time nodes, and uses this data to determine target perfusion parameters; and dynamically adjusts the perfusion operations of the hepatic artery and portal vein by combining real-time perfusion parameters and target perfusion parameters. The present invention determines the metabolic differences between the two circulations by performing thermal imaging recognition on the isolated liver and metabolic detection of the hepatic artery and portal vein circulations, effectively taking into account the metabolic balance of the two circulations, thereby improving the organ preservation lifespan and reducing organ tissue damage.

A perfusion loop assembly for ex vivo liver perfusion includes a pump providing perfusion fluid through a line branching at a branching point into a first branch line and a second branch line. The first branch line provides a first portion of the perfusion fluid to the hepatic artery of the liver, the first branch line coupled with a gas exchanger, where the first branch line includes a flow rate sensor and / or a pressure sensor. The second branch line provides a second portion of the perfusion fluid to the portal vein of the liver; the second branch line includes a valve for controlling flow of perfusion fluid into the portal vein. The second branch line includes a flow rate sensor and / or a pressure sensor. A liver chamber assembly holds the liver ex vivo, and an outlet line for the perfusion fluid connects the liver chamber assembly and the pump.

The present application discloses a liver perfusion system, comprising: an organ basin; a first circulatory tubing, comprising a first outflow tube and a first perfusion tube, the first outflow tube being configured for allowing perfusate to flow into the organ basin, and the first perfusion tube being configured for allowing perfusate to flow out from the first outflow tube; a second circulatory tubing, comprising a second outflow tube and a second perfusion tube, the second outflow tube being configured for allowing perfusate to flow into the organ basin, and the second perfusion tube being configured for allowing perfusate to flow out from the second outflow tube; a first tube connector, comprising a first opening, a second opening, and a third opening, the first opening being in communication with the first outflow tube, the second opening being in communication with the first perfusion tube, and the third opening being configured for communicating with a testing apparatus; and a second tube connector, comprising a fourth opening, a fifth opening, and a sixth opening, the fourth opening being in communication with the second outflow tube, the fifth opening being in communication with the second perfusion tube, and the sixth opening being configured for communicating with the testing apparatus. The liver perfusion system of the present application allows workers to easily perform sterility testing.

The invention relates to a separation method of rabbit primary hepatocytes. The method comprises the following steps: perfusing the liver of a rabbit, preparing a cell suspension from the perfused liver, and purifying the cell suspension to obtain primary hepatocytes; the perfusion liquid for perfusion contains calcium ions and enzyme. The rabbit liver perfusion method is developed in a targeted manner, the specific perfusion liquid is designed, it is ensured that the perfusion process is mild and effective, cell activity can be effectively maintained, cell damage can be effectively reduced, the cell separation efficiency and purity can be improved, in addition, the perfusion rate, time and the concentration of all components in the perfusion liquid are further designed, and the cell separation efficiency and purity can be improved. The cell separation efficiency and purity can be further improved.

The present invention provides a liver hypothermic machine perfusion preservation device and a method. The device includes a perfusion loop and a controller, wherein the perfusion loop includes a main pump, a main reservoir, an auxiliary pump and an auxiliary reservoir. The main pump is connected to an inlet of the main reservoir and configured to pump a perfusate from an organ cassette into the main reservoir; an outlet of the main reservoir is connected to a portal vein and a hepatic artery of a liver, and the main reservoir is configured to supply the perfusate to the portal vein and the hepatic artery; and the auxiliary reservoir and the auxiliary pump provide an additional hepatic artery perfusion flow path. According to the present invention, for a poor internal environment of the hepatic artery, the main reservoir and the auxiliary reservoir cooperate with each other to carry out perfusion, so that the perfusion efficiency and effect of the hepatic artery can be improved. The device according to the present invention also has a molecular-sieve oxygen generation function, and a free adjustment range of oxygen concentration from 21% to 90% can be achieved by means of combined control of a molecular-sieve oxygen generation channel and an air channel, thus providing a more flexible solution for the perfusion.

The invention relates to the technical field of medical equipment control, and discloses a multi-modal perception and controllable execution in-vitro liver perfusion system and a control method, monitoring data of at least two heterogeneous modals are collected and preprocessed, regulation and control parameters and uncertainty evaluation are reasoned and output through an edge intelligent terminal by using a first machine learning model, and the in-vitro liver perfusion system with the multi-modal perception and controllable execution function is obtained. And the data are uploaded to the cloud for further processing. The cloud generates a regulation result through a second machine learning model, adjusts the confidence of the cloud model based on the communication quality, and generates a to-be-executed regulation instruction in combination with local and cloud results, and when the network quality is lower than a threshold value, the system adopts a conservative regulation instruction to ensure the safety of the perfusion process; and the executability of the regulation and control instruction is ensured through security constraint verification, and manual intervention information is recorded. The in-vitro liver perfusion method can effectively cope with emergencies such as network instability and cloud failure while ensuring stable execution of the in-vitro liver perfusion process, and has relatively high robustness and reliability.

Methods are provided for effectively distinguishing the abilities of a human liver and an extracorporeal liver in an extracorporeal liver perfusion system to extract cholate and, thus, measure relative liver function.

The invention provides an in-vitro liver perfusion function evaluation method, device and medium based on hyperspectral imaging, a hyperspectral multi-index quantitative model of the liver in the normal-temperature mechanical perfusion process is established, and mapping calculation of oxygen saturation, hemoglobin, perfusion, moisture and hemoglobin index physiological parameters is achieved; spectral features are directly converted into functional parameters with clear physiological significance through a wave band absorption difference ratio and an integral reflection algorithm; a multi-index fusion model (LVI) is provided, and quantitative coupling between image parameters and metabolic parameters (lactic acid and pH) is achieved; a dynamic time sequence evaluation mechanism is formed, the activity change of the liver tissue can be tracked noninvasively, the function recovery trend can be predicted, and evaluation is provided for liver perfusion maintenance and subsequent liver transplantation.

Systems and methods are provided for collecting and processing data from liver cancer patients that can be used to personalize treatment plans for these patients, including by determining and considering their liver functional reserve LFR. Specifically, systems and methods are provided for using fLV (functional liver volume) and PHM (perfused hepatic mass), before and after treatment, to determine personalized treatment plans and improve overall survival rates.

The invention relates to the field of image processing, in particular to a liver perfusion regulation and control method and system based on image recognition, and the method comprises the steps: recognizing a thermal imaging image of an in-vitro liver during perfusion, obtaining temperature data, and calibrating an activity abnormal region of the in-vitro liver; identifying the change trend of an activity abnormal region of the hepatic artery and the portal vein, and estimating circulation degradation time nodes of the hepatic artery and the portal vein; according to the metabolic data associated with the cycle degradation time node, obtaining a metabolic difference so as to determine a target perfusion parameter; the perfusion operation of the hepatic artery and the portal vein is dynamically adjusted in combination with the real-time perfusion parameters and the target perfusion parameters. The in-vitro liver is subjected to thermal imaging recognition and hepatic artery and portal vein circulation metabolism detection, the metabolism difference between two cycles is determined, the metabolism balance of the two cycles is effectively considered, the preservation life of organs is prolonged, and organ tissue damage is reduced.

The utility model provides a blood storage device and a liver perfusion system, and the blood storage device comprises a tank body which is provided with a blood storage cavity and two blood outlets; the upper cover is arranged on the tank body in a covering mode and seals the blood storage cavity, the upper cover is provided with a blood inlet and at least one exhaust port, and the exhaust port is communicated with the atmospheric environment; and the filtering mechanism is arranged in the blood storage cavity, is positioned between the blood inlet and the blood outlet, and is used for filtering the blood entering from the blood inlet and discharging the filtered blood from the blood outlet. The design of the single blood inlet and the double blood outlets is adopted, the liver physiological structure can be matched, the exhaust port communicated with the atmospheric environment is arranged, the negative pressure in front of the centrifugal pump can be balanced, and therefore the problem that the inferior vena cava sucks flat cannot occur. The blood can be filtered through the filtering mechanism after entering the blood storage device, and the filtered blood flows out through the two blood outlets respectively, so that the risk problem caused by the fact that thrombus flows back to the liver is avoided.

Methods are provided for effectively distinguishing the abilities of a human liver and an extracorporeal liver in an extracorporeal liver perfusion system to extract cholate and, thus, measure relative liver function.

1. Name of the design product: Self-check guidance and parameter setting graphical user interface for a liver perfusion system used in an electronic device. 2. Use of the design product: For an electronic device. 3. Design points of the design product: Lie in the content of the graphical user interface on the screen of the electronic device. 4. Picture or photo that best shows the design points: Front view. 5. Use of the graphical user interface: The interface of this design product is used for data monitoring and control of the liver perfusion system. 6. Human-computer interaction method of the graphical user interface: The front view is the self-check page of the liver perfusion system; click "Confirm alarm prompt and next" below the front view to enter Interface Change State Diagram 1; click "Next" below Interface Change State Diagram 1 to enter Interface Change State Diagram 2; click "Next" below Interface Change State Diagram 2 to enter Interface Change State Diagram 3; click "Complete" below Interface Change State Diagram 3 to enter Interface Change State Diagram 4; click "Start zero calibration" below Interface Change State Diagram 4 to enter Interface Change State Diagram 5; click "Complete" below Interface Change State Diagram 5 to enter Interface Change State Diagram 6; in Interface Change State Diagram 6, click the switch buttons on the right side of "Water temperature °C", "Hepatic artery", and "Portal vein" in sequence to enter Interface Change State Diagram 7; click the setting button on the right side of "Rotation speed" below "Hepatic artery" in Interface Change State Diagram 7 (the position of the 2000 button to the right of 1970 in the illustration) to enter Interface Change State Diagram 8.

The invention relates to the technical field of biology, in particular to a mouse liver cell extraction and cryopreservation method. Comprising the following steps: performing liver perfusion and digestion: performing inferior vena cava intubation on a mouse corpse, cutting open vena cava, clamping superior vena cava, performing perfusion with perfusate for 6-8 minutes, and performing circulating perfusion with digestive juice for 10-15 minutes; separating and purifying the cells: taking out the liver, tearing a liver membrane in precooling termination digestion liquid at 3-5 DEG C, filtering by a screen, collecting filtrate, performing primary centrifugation on the filtrate, supplementing a complete culture medium, then performing secondary centrifugation, collecting cell precipitates, washing with the complete culture medium for 2-3 times, performing centrifugation again, and retaining the cell precipitates at the bottom to obtain purified parenchymal liver cells; and resuspending the purified parenchymal hepatic cells by using a precooled cryopreservation solution until the final concentration is (1-2) * 10 < 7 > cells / mL, sub-packaging and cryopreserving. The method is stable in operation, high in cell obtaining rate, good in activity and high in recovery rate after cryopreservation.

According to the remote monitoring system and method for the in-vitro liver perfusion equipment, medical staff can move and operate a tablet personal computer within a certain range in an operating room without constraint of the physical position of an equipment host, a patient and the equipment can be taken into consideration at the same time, it can be guaranteed that the equipment is normally controlled in a small space or in a multi-person operation scene, and the operation efficiency is improved. The flexibility of the working process is improved; through the Internet cloud, remote specialists can observe the whole perfusion process in real time as if they are personally on site, so that instant remote guidance is provided for evaluation of marginal donor liver and processing of complex conditions, and the utilization efficiency and the treatment level of medical resources are improved; the dual network architecture provides communication redundancy to a certain extent. Based on the alarm pushing function of the cloud platform, 7 * 24-hour state monitoring is realized, even if field personnel leave temporarily, key alarms can be known in time through mobile phones, and the safety is greatly improved.