38 results about "Liver perfusion" patented technology

The invention discloses an isolated liver perfusion system and method. The system comprises a perfusate temperature control tank, an isolated liver preservation tank, a portal vein perfusion path, a hepatic artery perfusion path, a perfusate backflow path and a bile collector. The portal vein perfusion path is used for conveying perfusate to the portal vein of an isolated liver, the hepatic artery perfusion path is used for conveying perfusate to the hepatic artery of the isolated liver, the perfusate backflow path is used for recycling perfusate flowing out of the isolated liver into the perfusate temperature control tank, and the bile collector is connected with the isolated liver preservation tank and used for collecting bile. The isolated liver is perfused from the portal vein and the hepatic artery through the two paths respectively, the system and method are applicable to damage protection in the isolated liver preservation process, the reperfusion injury of the liver can be significantly relieved, and reliability, stability and repeatability are high. The system and method can effectively store the isolated liver at low temperature, mild hypothermia and normal temperature, and the limitation that the isolated liver needs to be stored at low temperature and the requirement for marginal donor liver preservation cannot be met in the prior art is changed.

The invention provides a CT liver-perfusion image post-processing method and a CT liver-perfusion method. The CT liver-perfusion image post-processing method includes: obtaining a CT liver-perfusion image sequence and identifying an abdominal aorta from the CT liver-perfusion image sequence so as to obtain a time curve of a hepatic-artery contrast-agent concentration; and based on the time curve of the hepatic-artery contrast-agent concentration, calculating a contrast-agent concentration time curve of each pixel point and obtaining liver-perfusion parameters. The invention also provides a CT liver-perfusion method which uses the CT liver-perfusion image post-processing method. The CT liver-perfusion image post-processing method and the CT liver-perfusion method are higher in accuracy.

The invention discloses a liver perfusion device, which comprises a main machine, an air-oxygen mixer assembly and a monitoring assembly. A consumable assembly is clamped on a worktable of the main machine, and when different livers need to be perfused, only the consumable assembly needs to be replaced, so that convenience is achieved; the main machine is internally provided with a temperature adjusting assembly in pipeline connection with the consumable assembly, thus the temperature of circulating water entering a first lung membrane oxygenator and a second lung membrane oxygenator for heatexchange can be adjusted through the temperature adjusting assembly, and then liver perfusion can be conducted under different temperature conditions according to the actual needs; and in addition, the main machine has different liver perfusion modes, a user can set hepatic artery and portal vein perfusion methods according to the actual needs, the perfusate oxygenation degree is adjusted according to an air-oxygen mixer, different drugs are injected by a micro-injection pump, and the effects of liver long-term preservation, improvement, repair and the like are achieved.

The invention relates to a, especially a perfusion preservation device for liver. The perfusion preservation device for liver includes a water-bath case perfused with a preserving liquid. The device is characterized in that the inside of the water-bath case is provided with a bile duct perfusion tube connected to a bile duct, a hepatic artery perfusion tube in connection with a hepatic artery and a portal vein perfusion tube connected to a portal vein. The ends of the bile duct perfusion tube, the hepatic artery perfusion tube and the portal vein perfusion tube are all connected to a liquid storage pot through a pipeline. And the other end of the liquid storage pot is in connection with a pressure adjustable hydraulic pump that is connected to the water-bath case by a pipeline directly. Earmarked for liver perfusion preservation, the device of the invention is characterized by simple structure, convenient use, long storage and the like.

A simple in vitro liver perfusion apparatus for digesting and separating liver cell characterized in that a container is installed on the engine block, inside the container being positioned a stainless reticular baffle plate, a heating device being installed in the container bottom under the stainless reticular baffle plate, a perfusion bottle with a side hole is fixed on the top surface of stainless reticular baffle plate by a fixing clamp, and a stainless filter net is fixed on the top part by bracket. An input end of peristaltic pump on the engine block is connected with an aseptic input conduct, while the out put end of the peristaltic pump is connected with an aseptic outputting conduct, needle handle and perfusion needle. It can realize two step liver perfusion for in vitro liver, obtain effective perfusion for anterior perfusion fluid and digestive fluid rapidly and continuously in the largest extent, and monitor liver tissue process

The present invention relates to the field of extracorporeal liver perfusion and, more particularly, to the design of a chamber in which a biological component can be housed to form e.g. a bio-artificial liver (BAL). It also relates to a bio-artificial liver per se, it's components and methodological steps associated with its development and use. The chamber (10) for the biological component (100) of a bio-artificial liver (200) is configured to allow: • Proliferation of the biological component, in situ; • Cryopreservation of the biological component, in situ, and • Perfusion of the biological component, in situ.

The invention relates to the medicine field, especially relates to a perfusion device, which can rapidly carry out connection and perfusion when obtaining a supplied liver in an organism (liver) transplant operation, and specifically relates to a supplied-liver quickly-fixed perfusion device for liver transplant. The perfusion device comprises a puncture guiding device and a connecting ring for connecting a perfusion pipe. The puncture guiding device is a puncture steel wire, a contractible and magnetic ring is arranged on the top end of the steel wire, the top end of the connecting ring is in a conical shape, the bottom of the connecting ring is connected to a perfusion pipe, a unidirectional valve is arranged in the bottom of the connecting ring, the top of the connecting ring is made of an iron-containing material, the top of the connecting ring is matched with the contractible ring, and the top of the connecting ring and the contractible ring absorb on each other through magnetic force. The operation of the device is simple, practicable, and pertinent. The device is mainly used to carry out rapid perfusion when obtaining a supplied liver in a liver transplant operation, and is capable of rapidly and effectively performing liver perfusion so as to obtain a high quality liver.

The invention relates to the medicine field, especially relates to a perfusion device, which can rapidly carry out connection and perfusion when obtaining a supplied liver in an organism (liver) transplant operation, and specifically relates to a supplied-liver quickly-fixed perfusion device for liver transplant. The perfusion device comprises a puncture guiding device and a connecting ring for connecting a perfusion pipe. The puncture guiding device is a puncture steel wire, a contractible and magnetic ring is arranged on the top end of the steel wire, the top end of the connecting ring is in a conical shape, the bottom of the connecting ring is connected to a perfusion pipe, a unidirectional valve is arranged in the bottom of the connecting ring, the top of the connecting ring is made of an iron-containing material, the top of the connecting ring is matched with the contractible ring, and the top of the connecting ring and the contractible ring absorb on each other through magnetic force. The operation of the device is simple, practicable, and pertinent. The device is mainly used to carry out rapid perfusion when obtaining a supplied liver in a liver transplant operation, and is capable of rapidly and effectively performing liver perfusion so as to obtain a high quality liver.

The invention provides a simulation method and a quantitative test method of liver perfusion. The simulation method of liver perfusion includes the steps that a liver model is built, wherein the input of the liver model comprises at least one contrast agent concentration curve input to the liver model and weight coefficients corresponding to the contrast agent concentration curves, the contrast agent concentration curves are dominant parameters, and the weight coefficients corresponding to the contrast agent concentration curves are hidden parameters; a hepatic cell contrast agent concentration curve of the liver model is acquired according to different combinations of the hidden parameters and the dominant parameters and serves as the output of the liver model to simulate liver perfusion. The quantitative test method of liver perfusion is carried out based on the simulation method of liver perfusion. According to the simulation method and the quantitative test method of liver perfusion, cost is low, and operation is simple.

The invention relates to a system, a method and a device for controlling liver perfusion. The system comprises a first centrifugal blood pump subsystem and a second centrifugal blood pump subsystem, wherein the first centrifugal blood pump subsystem is connected with a liver artery and is used for carrying out pulse type blood supply; the second centrifugal blood pump subsystem is connected with a portal vein and is used for carrying out constant blood supply on the portal vein. A pulse blood pressure data treatment mode is carried out on liver artery perfusion line blood pressure, a constant blood pressure data treatment mode is carried out on portal vein perfusion line blood pressure, and a blood supply mode approximate to impulse blood supply of a real heart for the liver artery and a blood supply mode approximate to stable blood supply of a real portal vein in a human body for the portal vein are respectively generated by using blood pressure as a main control parameter and blood flow as an auxiliary monitoring parameter. The system, the method and the device for controlling the liver perfusion, disclosed by the invention, are beneficial for increasing the repairing and preserving effects on marginal donor organs such as liver and reducing a liver reperfusion injury risk.

An ultrasonic diagnostic imaging system and method acquire a sequence of image data as a bolus of contrast agent washes into and out of the liver. The image data of contrast intensity is used to compute time-intensity curves of contrast flow in the hepatic artery, the portal vein, and at one or more regions of interest in the liver. The time-intensity curve of contrast flow in the hepatic artery is scaled to the time-intensity curve of contrast flow in a region of interest to produce time-intensity curve data representing the flow of blood at the region of interest due to arterial flow. The time-intensity curve data representing the flow of blood at the region of interest due to arterial flow is subtracted from the time-intensity curve of contrast flow in the region of interest to produce time-intensity curve data representing the flow of blood at the region of interest due to venous flow.

The invention discloses a hepatic perfusion method used for separating liver lymphocytes of small animals, which is characterized in that after the animal is killed the perfusion is carried out by the vena cava posterior; the method of the invention has simple and quick operation, sufficiently removes the circulated blood, requires no special equipments, has low experiment cost, achieves the ideal hepatic perfusion effect, and is suitable for separating the liver lymphocytes of small animals such as rats, etc. for most laboratories.

The invention discloses a method for separating and preparing primary liver cells. In the method for separating and preparing the primary liver cells, composite enzymes are used for liver perfusion isolation to obtain the primary liver cells. The method belongs to the technical field of medical biology, the method for separating and preparing the primary liver cells can be used for effective separation of the primary liver cells, by interaction of the composite enzymes, different surface growth matrix of each cell can be fully hydrolyzed so as to obtain a single cell suspension. A suitable environment is provided for action of the composite enzymes by matching of a buffer system, so that enzymolysis is more complete, and the yield of the isolated primary liver cells is improved. A necessary environment is provided for cell life by catalytic properties of the composite enzymes and the buffer system, and the isolated primary liver cells have a higher survival rate.

The invention relates to a cell separation culture method of biological tissues and use thereof, in particular to a method for separating and culturing hepatic cells of a newborn rat (or mouse) and using the hepatic cells as a substitutional animal experiment model for toxicological evaluation of exogenous compounds. The method for separating and culturing the hepatic cells of the newborn rat (or mouse) of the invention has simple operation, does not need a liver perfusion process and expensive instruments, equipment and reagents, and can obtain more than 90% of liver parenchyma cells which have good uniformity and stability and high sensitivity and are easy for manual control. The hepatic cells separated and cultured by the invention are very sensitive to the toxicity of the exogenous compounds, have some functions and activity of in vivo hepatic cells, especially reserve the activity of drug metabolic enzyme, can well simulate the physiological environment of the in vivo liver, have the advantage of combining in vitro and integral animal toxicity tests, and can be expected to be used in high flux rapid detection of the toxicity of the exogenous compounds.

The invention discloses a liver perfusion consumable assembly. A sealed bionic storage device is arranged in a box body of the liver perfusion consumable assembly, so that the liver perfusion consumable assembly can meet the sterility requirement in the liver perfusion process; the bionic storage device is provided with an ice adding chamber, so that ice can be continuously added by the liver perfusion consumable assembly in the liver perfusion process; and besides, a liver suspending component is arranged in a storage chamber of the bionic storage device, so that a physiological environment for livers in the liver perfusion process is relatively close to that in a living human body.

The invention provides a CT liver-perfusion image post-processing method and a CT liver-perfusion method. The CT liver-perfusion image post-processing method includes: obtaining a CT liver-perfusion image sequence and identifying an abdominal aorta from the CT liver-perfusion image sequence so as to obtain a time curve of a hepatic-artery contrast-agent concentration; and based on the time curve of the hepatic-artery contrast-agent concentration, calculating a contrast-agent concentration time curve of each pixel point and obtaining liver-perfusion parameters. The invention also provides a CT liver-perfusion method which uses the CT liver-perfusion image post-processing method. The CT liver-perfusion image post-processing method and the CT liver-perfusion method are higher in accuracy.

The invention discloses an in-vitro liver perfusion device which comprises a support, a clamping mechanism, a hepatic portal vein perfusion channel and a hepatic artery perfusion channel, wherein the clamping mechanism is arranged on the support and is used for clamping a ligament of the in-vitro liver so as to hang the in-vitro liver; the hepatic portal vein perfusion channel is slidably arranged on the support, and one end of the hepatic portal vein perfusion channel is communicated with a hepatic portal vein of the in-vitro liver; the hepatic artery perfusion channel is slidably arranged on the hepatic portal vein perfusion channel, and one end of the hepatic artery perfusion channel is communicated with the hepatic artery of the in-vitro liver. The ligament of the in-vitro liver is clamped through the clamping mechanism arranged on the support, so that the in-vitro liver is hung, the in-vitro liver is in a free sagging state, the whole in-vitro liver is not extruded, and the situation that the liver function is damaged due to stacking of the in-vitro liver when the in-vitro liver is directly contained in a container is prevented; and the hepatic portal vein perfusion channel and the hepatic artery perfusion channel are arranged, so that a perfusion tube can be conveniently inserted and is not easy to fall off, and perfusate can be prevented from leaking at the insertion part.

The invention belongs to the technical field of animal liver perfusion for separation of liver cells and in particular relates to a liver perfusion method for separating rat liver cells and a perfusion apparatus of the method. In the method, according to a principle design of a classic two-step perfusion method, weight changes of rat livers are monitored in real time in a second step of enzyme liquid perfusion, and stop of the perfusion process is automatically controlled at the right time. The perfusion apparatus comprises a weighing platform, a perfusion system and a data processing system, wherein the perfusion system comprises a buffer solution tank, a peristaltic pump, a thermostat, a bubble trap and a puncture needle connected in sequence by virtue of infusion tubes; the weighing platform is electrically connected with the data processing system; rat livers are arranged on the weighing platform. The invention provides a perfusion apparatus capable of continuously monitoring liver weight changes in the perfusion process, and perfusion can be automatically stopped at the right time, so that viability and quantities of liver cells separated after liver perfusion are obviously improved, and the perfusion apparatus has significant substantive features.

The invention discloses a simple in-vitro liver perfusion device for digesting and separating liver cells. The simple in-vitro liver perfusion device comprises a liver tissue separation sterile box and a fixing mechanism, wherein the fixing mechanism is arranged in the liver tissue separation sterile box, the fixing mechanism comprises a left extrusion unit and a right extrusion unit with the samestructure, the liver tissue is placed between the two extrusion units, a puncture needle and a sleeve are inserted into the liver tissue, perfusate flows into the liver tissue from the puncture needle, the liver tissue is clamped through opposite movement of two extrusion units, meanwhile, multiple sets of expansion units are arranged on the sleeve outside the puncture needle, the expansion unitsare formed by movably connecting multiple pairs of supporting bodies with sliding rings capable of sliding through hinge points, when the two extrusion units get close to each other, the expansion units can automatically expand outwards under the action of thrust so as to clamp and fix the liver tissue till the liver tissue is completely separated, the separation time is shortened, and thereforethe liver tissue perfusion operation efficiency is further improved.

The invention provides a rat hepatic stellate cell separation method, which comprises the following steps of (a) injecting 10-percent chloral hydrate into the abdominal cavity for anaesthetizing a rat, and laparotomizing the abdominal cavity of the rat so as to expose the liver and the portal vein; (b) performing heparin intravenous injection, and preheating perfusion liquid for liver perfusion; (d) taking the liver subjected to sufficient perfusion, cutting the liver into pieces, and performing oscillation digestion in preheated digestion liquid; (e) filtering digested liver tissues through a multilayer sieve screen; performing low-speed centrifugation; sucking supernatant; performing centrifugation again to obtain cell precipitates; and performing once cleaning by cleaning liquid; (f) resuspending the obtained cell precipitates, and performing Nycoden gradient centrifugal separation; (g) cleaning the cells for many times, resuspending the cell precipitates by a complete culture medium, and performing inoculated culture; and (h) performing cell immunofluorescent identification on rat hepatic stellate cells. The rat hepatic stellate cell separation method provided by the invention can be used for obtaining rat hepatic stellate cells which have high yield, high purity, high activity and can be activated.

The invention discloses a liver perfusion consumable assembly. A sealed bionic storage device is arranged in a box body of the liver perfusion consumable assembly, so that the liver perfusion consumable assembly can meet the sterility requirement in the liver perfusion process; the bionic storage device is provided with an ice adding chamber, so that ice can be continuously added by the liver perfusion consumable assembly in the liver perfusion process; and besides, a liver suspending component is arranged in a storage chamber of the bionic storage device, so that a physiological environment for livers in the liver perfusion process is relatively close to that in a living human body.

The invention discloses an in-vitro liver perfusion activity maintaining device and a using method thereof. The in-vitro liver perfusion activity maintaining device comprises a box chamber, a liver inlet pipeline, a liver outlet pipeline and a bile duct pipeline. The box chamber comprises an outer-layer temperature control box and an inner-layer storage box arranged in the outer-layer temperature control box, the outer-layer temperature control box is used for controlling the inner-layer storage box to keep constant temperature, and the inner-layer storage box is used for storing an in-vitro liver; the liver inlet pipeline is used for being connected with the artery and / or portal vein of the in-vitro liver, the liver outlet pipeline is used for being connected with the inferior vena cava of the in-vitro liver, and the bile duct pipeline is used for being connected with the bile duct of the in-vitro liver.

The invention discloses a bionic perfusion method for a branch blood vessel model for liver perfusion. A mathematical simulation method is used for simulating a donor perfusion cleaning process before liver transplantation, and comprises the following steps: 1, determining specific dimensions and parameters of liver blood vessels at different branch angles according to medical image data; 2, constructing liver blood vessel models at different branch angles; 3, introducing intermittent double pulsating flow into the blood vessels, observing blood vessel kinetic phenomena generated in a perfusion process through medical measures, and determining blood vessel kinetic parameters, which are used for evaluating the blood vessel perfusion quality, according to the medical data; and finally, analyzing and comparing kinetic characteristic results obtained by perfusion of the branch blood vessels at different branch angles, and indicating that when the branch angles are about 60 degrees, a relatively good kinetic characteristic is realized. Therefore, a certain theoretical foundation is supplied to a mounting angle of a blood vessel stent of medical clinical application to relieving of hemadostenosis jam.

This application provides a whole-liver type bioartificial liver system dedicated pipeline, including a plasma separation pipeline, a blood storage tank, a blood storage tank drainage line, a pre-hepatic pipeline, a post-hepatic pipeline and a whole liver perfusion device; The pipeline includes a plasma separation catheter and a plasma separation pump, and the plasma separation catheter is connected with the blood storage tank to input recipient plasma; the blood storage tank drainage line includes a blood drainage catheter and a blood drainage pump, and the prehepatic pipeline includes a prehepatic catheter, liver The posterior pipeline includes the retrohepatic catheter, the blood drainage catheter, the prehepatic catheter and the retrohepatic catheter are connected sequentially in the direction from the output end of the blood storage tank to the input end, and the whole liver perfusion device is set between the anterior hepatic catheter and the retrohepatic catheter; The blood storage tank lead tube, pre-hepatic line, post-hepatic line and whole liver perfusion device constitute an extracorporeal liver perfusion cycle, and the plasma separation line and extracorporeal liver perfusion cycle are connected in parallel through the blood storage tank. The special pipeline provided by the present invention is adapted to the whole-liver bioartificial liver system, can realize the docking of various equipment in the system, and is easy to install.

The invention discloses a liver perfusion device, which comprises a main machine, an air-oxygen mixer assembly and a monitoring assembly. A consumable assembly is clamped on a worktable of the main machine, and when different livers need to be perfused, only the consumable assembly needs to be replaced, so that convenience is achieved; the main machine is internally provided with a temperature adjusting assembly in pipeline connection with the consumable assembly, thus the temperature of circulating water entering a first lung membrane oxygenator and a second lung membrane oxygenator for heatexchange can be adjusted through the temperature adjusting assembly, and then liver perfusion can be conducted under different temperature conditions according to the actual needs; and in addition, the main machine has different liver perfusion modes, a user can set hepatic artery and portal vein perfusion methods according to the actual needs, the perfusate oxygenation degree is adjusted according to an air-oxygen mixer, different drugs are injected by a micro-injection pump, and the effects of liver long-term preservation, improvement, repair and the like are achieved.

The invention discloses a gas delivery system for liver perfusion, the control module of which controls the work of the oxygen delivery control component and the compressed air delivery control component respectively according to the output results of the hepatic artery neural network training model and the portal vein neural network training model, so that the Based on the neural network training model and the preset oxygen partial pressure, carbon dioxide partial pressure and blood oxygen saturation, the real-time detection results of the hepatic artery detection module and portal vein detection module are trained respectively, and the gas in the hepatic artery and portal vein can be adjusted in real time and accurately delivery volume, so that the oxygen partial pressure, carbon dioxide partial pressure and blood oxygen saturation can be output stably according to the predetermined value; moreover, the bottom of the base is also equipped with universal wheels, so that the gas delivery system can be used and installed in different surgical sites ; In addition, when the gas storage capacity in the oxygen storage cylinder and the compressed air storage cylinder reaches a critical value, the relevant information can be notified to the operator in time through the alarm module.

The present invention discloses a temperature control system for liver perfusion. The system comprises a control module, a power module for supplying power to the temperature control system, a water tank for accommodating circulating water, a temperature regulator, a temperature monitoring component for monitoring the temperature of circulating water, and a centrifugal pump for driving circulatingwater into a pulmonary membrane oxygenator for heat exchange and returning the water to the water tank. The control module controls the temperature regulator to adjust the temperature of the circulating water according to the monitoring result of the temperature monitoring component, so that the temperature of the circulating water can be adjusted by using the temperature monitoring component andthe temperature regulator, and the temperature of the perfusate can be adjusted according to the temperature requirement of the liver perfusion.