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Constructing device and constructing method of artificial liver lobule functional unit

A functional unit and artificial liver technology, applied in the field of liver tissue engineering and bioartificial liver, can solve problems such as hindering the ability of material transmission and affecting the results of drug metabolism function testing

Inactive Publication Date: 2018-09-07
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the porous membrane will hinder the material transport ability between the two cells to a certain extent, and will also affect the results of drug screening and metabolic function testing

Method used

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  • Constructing device and constructing method of artificial liver lobule functional unit
  • Constructing device and constructing method of artificial liver lobule functional unit
  • Constructing device and constructing method of artificial liver lobule functional unit

Examples

Experimental program
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Effect test

Embodiment 1

[0041] See Figure 1-2 , The artificial liver lobule functional unit construction device of the present invention includes a liver plate basal layer 1, liver parenchymal cell area 2, and fluid channel layer 3 which are sequentially combined from bottom to top, and the liver plate basal layer 1 is processed with The patterned groove of the liver plate, the fluid channel layer 3 includes a fluid inlet channel 31, an endothelial cell oriented growth area 32, and a fluid outlet channel 33, and the liver parenchymal cell area 2 is laid in the patterned groove of the liver plate, The cell packing in the liver parenchymal cell area 2 is in a gel shape, and the top of the cell packing is directly exposed at the bottom of the endothelial cell directed growth zone 32.

[0042] An array of micropillars is arranged inside the patterned groove of the liver plate, the height of the micropillars 11 is between 10 μm and 1000 μm, the diameter of the micropillars 11 is between 10 μm and 500 μm, an...

Embodiment 2

[0053] Example 2 is basically the same as Example 1, except for the preparation method of collagen mixed with hepatocytes HepG2. Weigh 1g gelatin and 0.1g sodium alginate, pour them into the serum bottle, and use a pipette into the serum bottle Add 10ml of PBS solution, stir the material with a glass rod to make it as evenly mixed as possible, put the material in a 70°C drying oven, take it out and cool it for 30 minutes every 30 minutes, repeat 3 times. The concentration of the material is 10% (w / v). Add 100 μL of HepG2 plated hepatocytes to the collagen and stir thoroughly. The prepared collagen mixed with hepatocytes HepG2 is dripped onto the basal layer 1 (1) of the liver plate, scraped with a cell scraper, and then dripped with 1% (w / v) sodium alginate for curing, and the collagen is completely cured , Proceed to the next step.

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Abstract

The invention discloses a constructing device and a constructing method of an artificial liver lobule functional unit. The constructing device comprises a liver plate basal layer, a liver parenchyma cell zone and a fluid passageway layer, wherein a liver plate patterning groove is formed in the liver plate basal layer through processing; the fluid passageway layer comprises a fluid inlet passageway, an endothelial cell oriented growth zone and a fluid outlet passageway. The constructing method corresponding to the constructing device comprises the following steps: paving collagen containing liver parenchyma cells to the inner part of the liver plate patterning groove, bonding the liver plate basal layer with the fluid passageway layer after solidifying the collagen, pouring an endothelialcell suspension solution to the endothelial cell oriented growth zone, and enabling a cell culture medium solution to unidirectionally flow in the fluid inlet passageway, the endothelial cell orientedgrowth zone and the fluid outlet passageway after increasing endothelial cells to a certain amount. According to the constructing device and the constructing method, disclosed by the invention, reallayer-to-layer position relationship between endodermis and liver parenchyma cells is realized under dynamic culturing, the response of the liver parenchyma cells to physicochemical and biochemical irritation under the protection of the endodermis of blood vessels more tends to reality, and a more accurate result can be obtained when the artificial liver lobule functional unit is applied to drug screening, case research and therapy detection.

Description

Technical field [0001] The invention relates to the field of liver tissue engineering and bioartificial liver, in particular to a construction device and construction method of artificial liver lobule functional unit. Background technique [0002] The research of tissue engineering and regenerative medicine integrates the application of many fields including life science and engineering technology to manufacture functional human tissue. The development purpose of liver tissue engineering is to provide living donor transplantation or do liver function-related functional tests, drug metabolism and toxicity tests for patients with liver function impairment. The traditional construction method is through a top-down construction method, that is, liver cells are transplanted onto a polymer scaffold, and directed growth under the action of the cell matrix. However, this method cannot build a precise in vitro cell population on the microstructure. Relatively speaking, the bottom-up con...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/071
CPCC12M21/08C12M23/02C12M25/00C12N5/0671C12N2533/54
Inventor 弥胜利孙伟李柏翰李伟易晓满徐圆圆蒲海涛夏圣悦
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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