Artificial liver tissue and preparation method thereof

Abstract

The invention provides an artificial liver tissue and a preparation method thereof. The artificial liver tissue comprises a lower chip, a first microporous membrane, a middle chip, a second microporous membrane and an upper chip from the top to the bottom in sequence in a laminated manner. Three layers of cavities of a lower cavity, a middle cavity and an upper cavity are formed in the lower chip, middle chip and upper chip respectively. Three cavities are all grouted with culture solution containing growth factor, extracellular matrix containing hepatic parenchymal cells and suspension liquid containing endothelial cells. Adjacent cavities are separated by the microporous membrane. The microporous membrane can separate the matrix flow outside of the cell, and cause no influence to the cell migration. The culture solution and is fed through an inlet and an outlet of the upper cavity and lower cavity. Under the guidance of the growth factor, endothelial cells pass through the extracellular matrix of the middle cavity to form a lumen, and form the artificial liver tissue containing capillaries. The artificial liver tissue can simulate the human body liver tissue in a good way, and simulate the intrahepatic capillary functions of mass transfer, selective permeability, toxic reaction and the like.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to an artificial liver tissue and a manufacturing method thereof. Background technique [0002] At present, due to the lack of enough human donors and the large difference between animal-derived liver tissue and human-derived liver tissue, in order to meet the source requirements of human liver pathology research and drug detection and screening, the use of human-derived cells to construct in vitro liver models has been become a widely recognized method. [0003] Due to its advantages of small size, low consumption, high integration, good controllability, and the ability to construct complex structures, microfluidic chips have become one of the preferred solutions for constructing in vitro liver models. The commonly used microfluidic chip material is polydimethylsiloxane (PDMS), which has good biocompatibility, physical and chemical stability, light transmission and oxygen permeab...

AI Technical Summary

Problems solved by technology

Since the capillaries in the liver have many functions in the liver, such as material transfer, selective permeability, macromolecular toxicant screening and toxic reaction, for models lacking capillaries, whether it is used for pathological research of the liver or for drug detection screening, there are inaccurate problems

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