Reciprocally turning perfusion bioreactor for biological artificial liver and processing method thereof

Abstract

The invention discloses a reciprocally turning perfusion bioreactor for biological artificial liver and a processing method thereof. The invention discloses a bioreactor, which comprises a cylindrical container, wherein the upper and lower ends of the circumferential side wall of the container are both provided with a liquid inlet, and the two end parts of the container both have a liquid outlet. Pressure buffering rings are arranged at the positions of the liquid inlets in the container respectively, and cell screens are arranged at the positions of the liquid outlets at the two ends parts in the container respectively. The invention also provides a reciprocally turning perfusion bioreactor for biological artificial liver, which has the bioreactor, and a processing method thereof. The bioreactor has the advantages that: liver cells cultured on a micro carrier are uniformly distributed in the entire bioreactor; and in a reciprocating motion, the bioreactor causes little damage to the liver cells, allows liver failure plasma to be contacted fully for effectively avoiding perfusion dead cavity and ineffective perfusion, can detoxify plasma with high efficiency, is simple in operation, low in cost and remarkable in effect, and allows the biological artificial liver to play a treatment effect to a maximum degree.

Description

technical field [0001] The invention belongs to the technical field of biomedical equipment, in particular to a reciprocating flip-perfusion bioreactor for a bioartificial liver and a processing method thereof. Background technique [0002] Bioartificial liver or hybrid bioartificial liver uses part of the functions of liver cells in the bioreactor to biotransform toxic substances in the plasma of patients with liver failure on the one hand, and to secrete a variety of bioactive substances into the blood of patients on the other hand. In order to achieve the balance of improving the homeostasis of the patient. Bioreactor is the core part of bioartificial liver or hybrid bioartificial liver, and is the main place where hepatocytes detoxify the patient's plasma and secrete active substances. Therefore, as an efficient reactor, it should have the following characteristics: hepatocytes should react with The liquid in the bioreactor has a sufficient contact time, the liver cells...

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Problems solved by technology

[0003] At present, the hollow fiber bioreactor is the most widely used, but the disadvantages of the hollow fiber reactor are uneven cell distribution, poor cell adhesion, membrane fouling and clogging, and cell growth or excessive gas generation will destroy the fiber, both Affect the function of cells and affect the observation of the functional state of cells in the bioreactor

[0004] In addition, the currently used perfusion cylindrical bioreactor adopts a single inlet and a single outlet, the liquid is directly perfused, the shear force is large, and there is a perfusion dead space at a normal flow rate, or even ineffective perfusion. When the flow rate is high, the perfusate The shear force is high, and the liver cells are easily damaged, which affects its detoxification function of plasma

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