Filling bracket perfusion type bioreactor for artificial liver

Abstract

The invention discloses a filling rack perfusion biological reactor of artificial liver in the medically biological technical domain, which is characterized by the following: the appearance is cylinder shaped container; the liquid outlets 1 and 2 are upper and lower ends of the cylinder container; the outlet 3 and 4 are distributed in the center of lateral wall symmetrically; the physical rack 6 has 14-16 biological racks as crossing arrangement to deposit separation area and support liver cell; the liquid buffer area 7 is set in the middle of the inner rack of the reactor; setting cell screen and cell screen brush at outlet; connecting the upstream of two outlets of biological reactor with membrane oxygenator 14; connecting the downstream of two outlets with immune adsorber 15; the biological rack of the liver cell is chitose rack or alginic acid / chitose and modified alginic acid / galactose chitose / heparin rack; possessing good biological compatibility; fitting for growing liver cell and developing the function; avoiding collapse of the biological rack of the liver cell.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a medical device, a core device for the treatment of a biological or hybrid biological artificial liver-a artificial liver filling stent perfusion bioreactor. Background technique [0002] The bioartificial liver uses part or all of the bioactive cells of the liver cells to enter the bioreactor, and the blood or some components flow through the reactor. During this process, the bioactive cells absorb the toxic substances in the blood, Transformation and detoxification, on the other hand, synthesize a variety of biologically active substances and release them into the blood to achieve the purpose of removing toxic components in the blood. The bioreactor is the core device of the biological and hybrid artificial liver support system. It is the place where exogenous liver cells and the patient's blood or plasma exchange substances. As a reactor, it generally meets the following ba...

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Problems solved by technology

[0003] At present, the most widely used one is the hollow fiber reactor, but the disadvantage of the hollow fiber reactor is that the cells are not evenly distributed, and the cell adhesion is poor. Metabolic function, and it is easy to cause blockage of semi-permeable membrane, which is not conducive to material exchange

The perfusion scaffold type reactor can show its unique advantages in improving the efficiency of material exchange, which is particularly important for the function of the bioreactor, and the material requirements for the scaffold used in the bioreactor of the artificial liver must have: biocompatibility Good, easy to cast into various shapes, suitable mechanical and physical properties, can promote cell adhesion and mutual signal transmission, etc., the cell scaffold materials used in traditional packed bed scaffold reactors (polyethylene resin, polyurethane foam, etc.) The biocompatibility is not good, and the cell inoculation is not easy to be uniform, the single inlet mechanical liquid flow passes through the scaffold, the shear force is large, and the bigger disadvantage is that the scaffold packed bed scaffold reactor may also exist during liquid perfusion Ineffective perfusion and perfusion dead space make it difficult to ensure the nutrients in the center and edge of the entire stent reactor and die. These contradictions are more prominent after the stent reactor is enlarged, and there are still limitations in application. When compatible with better chitosan scaffolds and alginic acid / chitosan scaffolds, due to insufficient mechanical strength, cell scaffolds are prone to collapse, which is not conducive to the function of scaffold-type reactors, so how to improve or overcome the above shortcomings It is the key to constructing a new type of support type reactor

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