Filling bracket perfusion type bioreactor for artificial liver

Abstract

The invention belongs to the technical field of medical biology, and relates to a perfusion type bioreactor for filling a scaffold for an artificial liver. The appearance is a cylindrical container, the liquid inlets 1 and 2 are at the upper and lower ends of the cylindrical container; the outlets 3 and 4 are symmetrically distributed in the center of the side wall; the physical support 6 has 14 to 16 staggered partitions to place and support the liver cell biology The bracket 5 has a liquid flow buffer 7 in the middle of the bracket in the reactor, and a cell screen and a cell screen brush are arranged at the outlet. The upstream of the two inlets of the bioreactor is connected to the membrane oxygenator 14 , and the downstream of the two outlets is connected to the immunoadsorber 15 . Liver cell bioscaffolds are chitosan scaffolds or alginic acid / chitosan and modified alginic acid / galactosylated chitosan / heparin scaffolds, which have good biocompatibility and are more suitable for the growth and function of liver cells . The physical scaffold with a staggered structure is similar to each functional unit of the liver, effectively preventing the collapse of the liver cell biological scaffold.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a medical device, a core device for the treatment of a biological or hybrid biological artificial liver-a artificial liver filling stent perfusion bioreactor. Background technique [0002] The bioartificial liver uses part or all of the bioactive cells of the liver cells to enter the bioreactor, and the blood or some components flow through the reactor. During this process, the bioactive cells absorb the toxic substances in the blood, Transformation and detoxification, on the other hand, synthesize a variety of biologically active substances and release them into the blood to achieve the purpose of removing toxic components in the blood. The bioreactor is the core device of the biological and hybrid artificial liver support system. It is the place where exogenous liver cells and the patient's blood or plasma exchange substances. As a reactor, it generally meets the following ba...

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Problems solved by technology

[0003] At present, the most widely used one is the hollow fiber reactor, but the disadvantage of the hollow fiber reactor is that the cells are not evenly distributed, and the cell adhesion is poor. Metabolic function, and it is easy to cause blockage of semi-permeable membrane, which is not conducive to material exchange

The perfusion scaffold type reactor can show its unique advantages in improving the efficiency of material exchange, which is particularly important for the function of the bioreactor, and the material requirements for the scaffold used in the bioreactor of the artificial liver must have: biocompatibility Good, easy to cast into various shapes, suitable mechanical and physical properties, can promote cell adhesion and mutual signal transmission, etc., the cell scaffold materials used in traditional packed bed scaffold reactors (polyethylene resin, polyurethane foam, etc.) The biocompatibility is not good, and the cell inoculation is not easy to be uniform, the single inlet mechanical liquid flow passes through the scaffold, the shear force is large, and the bigger disadvantage is that the scaffold packed bed scaffold reactor may also exist during liquid perfusion Ineffective perfusion and perfusion dead space make it difficult to ensure the nutrients in the center and edge of the entire stent reactor and die. These contradictions are more prominent after the stent reactor is enlarged, and there are still limitations in application. When compatible with better chitosan scaffolds and alginic acid / chitosan scaffolds, due to insufficient mechanical strength, cell scaffolds are prone to collapse, which is not conducive to the function of scaffold-type reactors, so how to improve or overcome the above shortcomings It is the key to constructing a new type of support type reactor

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