Establishment method of turbot hepatic parenchymal cell line and turbot hepatic parenchymal cell line

Abstract

The invention relates to an establishment method of turbot hepatic parenchymal cell line and the turbot hepatic parenchymal cell line. The establishment method of the turbot hepatic parenchymal cell line comprises the following steps: obtaining turbot liver tissue; cutting the liver tissue into tissue blocks, and carrying out washing; carrying out digestion treatment by adopting type 4 collagenase, and carrying out washing again; carrying out digestion treatment on the tissue blocks obtained after the digestion treatment by adopting trypsin again; adding a primary complete medium to terminate digestion to obtain treated tissue blocks; uniformly attaching the treated tissue blocks into a culture bottle, placing the attached culture bottle into an incubator at 23 DEG C for 6 hours; inverting the culture bottle for 6 hours; adding 4 ml of a primary complete culture medium after the culture bottle is upright; performing primary culture; and then, performing subculture. The turbot hepatic parenchymal cell line established by the method can realize continuous passage so as to provide a large number of turbot hepatic cells; and the established stable and sustainable turbot hepatocyte line can be applied to the molecular cell biology research of turbot nutrition metabolism, thereby overcoming defects in the prior art at the cellular level.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a method for establishing a turbot hepatic parenchymal cell line and the cell line. Background technique [0002] The liver is one of the most important organs in the body, and it has many important functions such as nutrient metabolism and immune regulation. In the liver, hepatic parenchymal cells are the most important cell type in the liver, and have important functions such as storage of glycogen, protein metabolism, and fat metabolism. In the study of aquatic animals, the liver of fish is used in the research of growth, immunity, toxicity and other experiments. However, due to the large individual differences of aquatic animals, the data obtained in scientific research often have large differences within the group, and there are differences between the experimental results and the real situation. Therefore, the application of hepatocyte culture in aquatic ...

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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to overcome the deficiencies of the prior art, and provide a method for establishing a turbot hepatic parenchymal cell line and a cell line, so as to solve the problem that the prior art cannot obtain continuous passage and maintain a good growth state. Problems with turbot hepatocytes

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