The invention relates to the technical field of needle mushroom cultivation, in particular to a cultivation method of bottle planted needle mushroom. The cultivation method includes three steps, namely budding, restraining and growing. In budding, a control and regulation system with temperature, illumination and carbon dioxide concentration as the features and ventilation quantity as the whole selects appropriate parameter values to regulate and control needle mushroom hyphae. In restraining, wind restraining, light restraining and temperature restraining are organically combined to control growing height, stipe toughness and pileus size of sporocarps. In growing, carbon dioxide concentration is improved, and the needle mushroom with uniform and moderate pilei and vertical stipes can be obtained. The cultivation method reasonably uses illumination, temperature, humidity and carbon dioxide concentration to meet growth requirements of the needle mushroom at different stages by combining the growth characteristics of the needle mushroom, proportionally controls the whole production process, stabilizes product quality, enables the cultivated needle mushroom to be good in quality, stable in quality and consistent in growing, and enables the pilei of the needle mushroom to be uniform and moderate.