48 results about "Sarcocheilichthys sinensis" patented technology

The invention belongs to the field of aquiculture, and relates to a method for breeding crabs and controlling diseases. The method provides an original biological environment with the best surviving and growing conditions for natural crabs, and obtains high quality Chinese Eriocheir sinensis as a crab seed by backcross and purification and rejuvenation. Breeding of the crab is returned nature by creating the original biological environment of crab survival, namely a natural crab pond, and carrying out a scientific and biological breeding method; and original biological environment and range suitable for crab growth are set, such as requirement on original biology of the crab pond, water quality, water temperature, breeding density, scientific bait dosage, water seed coverage rate and the like, and the unhealthy method which uses antibiotics, hormones and other medicaments and industrial synthetic feeds in a breeding process is eliminated, so that the bred crabs are big and strong, has rich cream, delicious meat and rich smell of wild crabs with average specification of between 150 and 250 grams. The crab can meet the requirements on export and pollution-free food, has high economic value and good social benefit.

The intensified cultivation and fattening technology of Chinese mitten crabs involves aquaculture technology, and is characterized in that: the adult crabs of Chinese mitten crabs that have moulted for the last time are selected as breeding and fattening objects, and the male and female are separated and put into fattening ponds respectively. After the crab enters the fattening period, it is mainly in the stage of fattening and gaining weight. The combination of plant-based concentrate feed and animal-based bait is fed once in the morning and evening, and the daily feeding amount generally accounts for 3-4% of the body weight of the mitten crab. Invention of Chinese mitten crabs through intensive cultivation, due to the plump fat and muscle filling, has enough energy to make the fattened Chinese mitten crabs pass the overwintering stage until April-May of the next year, so that through strengthening measures and corresponding supporting technologies, it can The market time of Chinese mitten crabs is greatly extended, and the balanced listing of Chinese mitten crabs is basically achieved, and Chinese mitten crabs with plump fat, muscle filling and delicious taste can be obtained, and the quality of Chinese mitten crabs can be improved in a short period of time. Get high benefits in a short time.

The invention discloses an ecological breeding method of Eriocheir sinensis. The method comprises the steps that a pond is prepared firstly, an annular groove, an anti-permeable membrane and an inlet and outlet water pipe with a filtering mesh are arranged, water is poured after exposure, water plants are planted and loaches and diatom are placed in the pond after disinfection,, a biological agent and chicken manure are scattered and fertilized after water adding, the temperature, the dissolved oxygen and the PH value of the water are adjusted, the breeding pond is obtained, young Eriocheir sinensis is thrown into the breeding pond after being sterilized, feeding is carried out according to different growth periods of the Eriocheir sinensis, plant ash, the biological agent and inorganic salt type nutrient substances are scattered and fertilized regularly, and periodic water cleaning and disinfecting are carried out. According to the ecological breeding method, the environment and nutrient substances needed in the growth period of the Eriocheir sinensis can be provided, fast growth of the Eriocheir sinensis can be promoted, the specification and yield of the Eriocheir sinensis can be improved, and the method is ecological, environmentally friendly and economical.

The invention relates to a directional mating and breeding method in Eriocheir sinensis breeding, comprising the following steps of: referring to the Eriocheir sinensis standard GB/T19783-2005, selecting male and female Eriocheir sinensis individuals with mature gonads from different species groups in the first ten-day period of November as parents, after labeling, breeding the male and female Eriocheir sinensis individuals separately, and strengthening cultivation; in the last ten-day period of November, pairing and putting the parents subjected to cultivation strengthening into cylindrical net cages weaved by polyethylene soft plastic, wherein each cylindrical net cage is 50 cm in diameter and 60 cm in height, and the salinity of water is 0.209; distributing a male crab for each net cage, wherein the ratio of female to male of full sib directional mating is 1:1, and the ratio of female to male of half-sib directional mating is 2: 1 or 3: 1; putting the obtained egg-carrying crabs into a 2*667 m<2>*1.5 m outdoor soil pond for overwintering cultivation; in the middle ten-day period of April of next year, pumping out the water in the pond to catch the egg-carrying crabs, putting the egg-carrying crabs into cylindrical net cages weaved by polyethylene soft plastic, wherein the cylindrical net cages are respectively 20 cm in diameter and 50 cm in height and are respectively arranged in net boxes in size of 3*2*1.5 m in other soil ponds respectively, and one egg-carrying crab is arranged in each net box; after the culturing of young crabs is finished, taking out the parents, and performing soil pond cultivation for the juvenile crabs according to the Eriocheir sinensis artificial breeding standard SC/T1099-2007.

The invention relates to a breeding technology for large-size parents of Eriocheir sinensis. The breeding technology comprises the step of selecting the athletic parents of the Eriocheir sinensis, wherein the size of the female crab is above 150g and the size of the male crab is above 200g; the breeding technology is characterized by comprising the following steps: from the last ten-day of October to the first ten-day of November per year, soaking the selected parents of the Eriocheir sinensis into fresh water with tiny running water and sufficient oxygen for 2-3 minutes, and then taking out; after 3-5 minutes, disinfecting by using potassium permanganate; temporarily breeding in an indoor glass reinforced plastic over-wintering pool; starting the over-wintering breeding for the parents of the Eriocheir sinensis in the middle ten days of December; at the beginning of March per year, at a mating moment, controlling the water temperature at 9-12 DEG C, wherein the male and female ratio is (1.5-2):1 and the mating density is 7-13 pieces/m<2>; after oogenesis, continuously aerating and increasing the inflating volume, wherein the breeding temperature for the larva of the Eriocheir sinensis is at 24-25 DEG C and the breeding seawater salinity is 1.8%; and feeding rotifera, fairy shrimp larva and fresh water cladocerans biological baits.

The invention discloses a method for breeding Eriocheir sinensis in an indoor water tank. The method comprises the steps: (1) construction of a breeding environment in the indoor test water tank; (2) selection of Eriocheir sinensis; (3) realization of reasonable stocking density; (4) daily management and bait feeding; and (5) management during molting. The method for breeding the adult Eriocheir sinensis in the indoor water tank is developed by combining ecological breeding engineering and aiming at breeding in the indoor water tank according to the characteristics of the breeding biology and the autecology of the Eriocheir sinensis. Proved by the technical practices of the inventor, the adult Eriocheir sinensis breeding in the indoor water tank succeeds, and the method has great significance in the field of breeding and cultivating Eriocheir sinensis.

The invention discloses an Eriocheir sinensis sexual precocity property related SNP marker and its application. The SNP is positioned in a 3'-UTR region of ecdysis inhibition hormone gene MIH(AY310313.1) and is concretely positioned in 3088bp, and base variation information is SNPA3088T T>G. Property-genotype relevance analysis results show that a significant correlation exists between SNP mutation and river crab sexual precocity properties, wherein the sexual precocity property risk of the GG genotype river crab individuals is 2.175 times higher that of TT genotype individuals. The polymorphism SNP marker of the river crab sexual precocity related gene, and can be used for the subsequent and river crab sexual precocity property related genetic and breeding researches.

The invention relates to the field of water purification, in particular to a method for managing pool eutrophication by planting rice. The method for managing pool eutrophication by planting the rice includes the following steps: a pool is aired and cleaned, and pests in the pool are killed; organic fertilizers for fermentation treatment are applied in middle of March; food organisms are started to be cultivated at the beginning of April; the food organisms are placed into the pool; reed rice seedlings are started to be cultivated at the beginning of May; rice seedling transplanting is started to be carried out in the middle of May; urea is applied after rice seedling transplanting is carried out; large-specification juvenile eriocheir sinensis, odontobutis obscura fries and an appropriate number of aristichthysnobilis fishes are input in the middle of June; compound fertilizers are applied at the beginning of July; harvesting is started to be carried out in the middle of November. By means of the method, rice is planted in the pool, the eriocheir sinensis and the odontobutis obscura which are high in economical value are bred in a mixed mode, aquatic products which are high in quality and high in economic value are produced while the water is purified, the price is higher the price of river crabs in the pool, ecological rice is achieved, and the pool cultivation comprehensive benefit is further improved.

The invention discloses an SNP mark related to the weight of eriocheir sinensis and application of the SNP mark. The SNP mark related to the weight of the eriocheir sinensis is located on the5'- side wing area of an MIH gene, the specific position is the 372bp part of the MIH gene, and nucleotide variation information is SNP g.372T>C. Obvious relevance exists between the SNP position point and the weight trait of the eriocheir sinensis species, and the average weight of the CC gene type individual is obviously higher than the average weight of the TT gene type individual. SNP g.372T>C genetic typing is carried out on a river crab breeding colony and morphology characteristic analysis is combined so that the individual with the gene type of CC can be preferably selected as a breeding parent, and the research has the significant guiding meaning on breeding of a novel large-specification river crab variety.

The invention relates to a large-scale family juvenile crab soil pool culture method for eriocheir sinensis. The method is characterized by comprising the following steps that firstly, a soil pool with good water body flowability is selected to be used as a culture pool; secondly, bolting silk net piece is used for cutting the soil pool into a plurality of partition spaces which do not influence the water body flowability; thirdly, each family oogenesis female crab is singly placed into a cage, then, a cage containing the female crab is hung in the corresponding partition spaces, the water temperature and the seawater salinity are controlled, the water body flowability is maintained for young crab releasing; fourthly, the cage containing the female crab is moved away after the young crab releasing, the family larva culture is carried out until the larva grows into megalopa; and finally, the water desalting and the final night lamp light trapping are carried out in the original partition space, and the juvenile crab culture is completed. The method provided by the invention has the advantages that the requirement on a hatching pool is low, but the larva production environment is directly connected with the pool bottom, the pool bottom microbe purification capability is good, and the production environment is excellent, so few pools can be used for producing more family juvenile crabs, and in addition, the emergence rate and the survival rate of each family are greatly improved.

The present invention discloses molecular genetic marker method for detecting and identifying plasm germ of Eriocheir sinensis (river crab). The detecting and identifying process includes extracting DNA of river crab; and performing PCR amplification with the DNA as template and primer TGACCCGCCT (SEQ ID No. 1) and amplification product analysis. When the 947 bp band appears in the frequency of 80-100 %, the sample is Yangtse River crab; and when the 947 bp band appears in the frequency of 0-20 %, the sample is Liaohe River crab. The method is used in distinguishing Yangtse River crab from Liaohe River crab fast. The present invention also provides corresponding detection reagent kit.

The invention discloses an SNP locus significantly relevant to the precocious puberty trait of eriocheir sinensis and the application. The SNP locus is positioned in a Vg gene 5'-flanking region-437bpposition of the eriocheir sinensis, and nucleotide variation information is SNP g.-437T) G. PCR primer used for detecting the SNP locus is SEQ ID NO.1 and SEQ ID NO.2, and restriction enzyme is MboI.The SNP locus has significant correlation with precocious puberty of the eriocheir sinensis, and the risk of precocious puberty trait of GG gene eriocheir sinensis individual is obviously smaller than that of precocious puberty trait of TT gene individual. The SNP locus and the PCR primer used for genetic typing are applied to the predication of the risk of precocious puberty of the eriocheir sinensis and/or the variety breeding of the eriocheir sinensis.

The invention relates to a fish feed, and particularly relates to a feed for preventing fin rots of sarcocheilichthys sinensis, and the invention further relates to a preparation method of the feed for preventing the fin rots of the sarcocheilichthys sinensis. The feed for preventing the fin rots of the sarcocheilichthys sinensis comprises the following components in parts by mass: 20 to 30 parts of fish meal, 8 to 15 parts of earthworm powder, 4 to 10 parts of squid powder, 6 to 12 parts of rice bran, 3 to 7 parts of houttuynia cordata, 3 to 8 parts of honeysuckle, 2 to 6 parts of radix clematidis, 3 to 7 parts of cassia twig, 2 to 5 parts of grapefruit, 2 to 6 parts of aster, 1 to 5 parts of asparagus cochinchinensis, 1 to 4 parts of cuttle bone, 2 to 6 parts of purple perilla, 1 to 4 parts of purslane, 1 to 4 parts of olive oil and 1 to 4 parts of compound vitamin. The feed for preventing the fin rots of the sarcocheilichthys sinensis is provided according to energy metabolism modes and nutritional requirements of the sarcocheilichthys sinensis; after the sarcocheilichthys sinensis is fed, the feed coefficient is low, the specific growth rate is high, and the superficial ulcer resistant capability is strong.

The present invention relates to a method fox mixed-raising crab and clam. It is characterized by that in every ma of water surface in which 1000 breeding clams are being bred 5-8 kg of juvenile crabs can be bred. Besides, said invention adopts special-purpose bag for raising breeding clams, every bag can hold 3-4 breeding clams, and regularly adds food and changes fresh water, and regularly spread quicklime powder so as to attain the goal for mixed-raising clams and crabs.

The present invention relates to a method for ecological breeding of Chinese mitten crabs, comprising the following steps: firstly, determining a pond for the cultivation of Chinese mitten crabs; secondly, planting aquatic plants in the breeding ponds every January-February; thirdly, 2-2 In March, stock Chinese mitten crab species in the breeding pond; fourth, in late March, feed animal bait into the breeding pond; fifth, in early April, start to put snails in the breeding pond; sixth, 4 In the second ten days of the month, set up insecticidal lamps in the breeding ponds; seventh, in May-September, apply EM bacteria to the breeding ponds; eighth, in the first ten days of August, start to add plant bait to the breeding ponds; ninth, in August In the second half of the year, the animal bait was added to the breeding ponds. The invention greatly reduces the input of compound pellet feed and other baits, significantly reduces the cost of production input, supplements EM bacteria to eliminate toxic and harmful substances, realizes only water intake without changing water, and does not apply any drugs, basically realizing The breeding of pollution-free high-quality Chinese mitten crabs.

The invention discloses a caring method for waterweeds in Chinese mitten crab cultivating pond. By utilizing the principle that the waterweeds are firmly rooted in the pond and can not die because the roots of the waterweeds are taken off, the caring method can make the waterweeds can not die caused by detached roots and deficiency in nutrients in summer with high temperature and higher water level through controlling the water level and applying fertilizer. The caring method disclosed by the invention is characterized in that the growth of the waterweeds is controlled by the water level, andthe growth of the roots of the waterweed is promoted by applying a certain ratio of fertilizer, so that the caring method can make the rooting of the waterweeds firm and prevent the floating roots ofthe waterweeds from dying so as to keep the waterweed alive in the summer. In addition, the excessive waterweeds around the pond can be cut so as to ensure the sufficient supplement of the fertilizerfor the growth of the waterweeds and make the Chinese mitten crab capable of being fed easily on the bank. The caring method disclosed by the invention can care the waterweeds by controlling the water level and reasonably applying the fertilizer so as to make the waterweeds alive in summer and all the other seasons, improve the water quality, and reduce the negative effects of high temperature insummer to the growth of the Chinese mitten crabs, thereby providing the basis for ecologically and efficiently cultivating Chinese mitten crabs.

The invention relates to an intercropping ecological breeding method of Siganus guttatus and Eriocheir Sinensis. The method comprises the following steps: (1) a pond is cleaned firstly before the water enters the pond, the pond is aired for 18-20 days before the water enters the pond, and the salinity of the water is 2-5; (2) aquatic plants are planted around the pond and in the middle of the pond, and then Eriocheir Sinensis juvenile crabs are put in the pond, after two months, the Siganus guttatus and Eriocheir Sinensis are bred in an intercropping mode, and a special compound feed for the Eriocheir Sinensis is fed for two times every day. According to the breeding method disclosed by the invention, after the pond is cleaned, and the average weight of the eriocheir sinensis in the pond where the Siganus guttatus is also intercropped is more than 320 g, and the average weight of the eriocheir sinensis is increased by more than 6.2% compared with that of the Eriocheir sinensis which isnot bred in the pond where the Siganus guttatus is also intercropped; and the yield of the Eriocheir sinensis in the pond where the Siganus guttatus is also intercropped is 150 kg per mu and the yield of the Eriocheir sinensis is increased by more than 19.6% in comparison with the yield of the Eriocheir sinensis which is not bred in the pond where the Siganus guttatus, the breeding effect is remarkable, and the application prospect is good.

The present invention discloses an in-vivo nondestructive sampling method for eriocheir sinensis and an application thereof. Fertilized eggs of egg-carrying parents of eriocheir sinensis are sampled,and DNAs and RNAs are extracted from the fertilized eggs for detection of a relevant pathogen. The parents free of the detection object pathogen are segregated for breeding, and then specific pathogenfree (SPF) megalopae of eriocheir sinensis are obtained through captive breeding. The method has the advantages that the bodies of parents are not injured by sampling, thereby guaranteeing normal eggcarrying and hatching of the parent crabs, and the obtained megalopae of eriocheir sinensis are specific pathogen free. The in-vivo nondestructive sampling method for eriocheir sinensis solves the difficult problem of in-vivo nondestructive sampling in SPF breeding of eriocheir sinensis and provides a simple and feasible approach for SPF breeding of eriocheir sinensis. Thus, healthy SPF seeds areexpected to be bred, so that the economic benefit of breeding of eriocheir sinensis can be increased.

The invention discloses an ecological breeding method for interbreeding Australian freshwater lobster and Eriocheir sinensis, which is characterized by comprising the following steps: (1) cleaning andsterilizing a pond, and starting to feed water after sunning the pond for 10 to 15 days; (2) planting bitter grass and sporochloa rosea in circumferential ditches on the periphery of the pond, and planting eolian grass on a middle platform; then putting Eriocheir sinensis in February to March, wherein the density is 600-1200 per mu; putting base feed snail in March, 200-400 kg/mu; (3) after May,the water temperature in the pond is stabilized above 20 DEG C, putting the freshwater lobster fries of Australia in the pond, and the density is 500-1500 per mu, The specification of the fries is more than 3g; (4) feeding Eriocheir sinensis feed once a day at 7:00 and 17:00 respectively, wherein the feeding amount per day is 5%-8% of the weight of the Eriocheir sinensis; after July, opening the aerator at 22:00-6:00 every day. According to the breeding method, the survival rate of the Eriocheir sinensis is improved, the yield per mu is improved, and the economic benefit is improved.

The invention discloses a storage device for preventing fighting of eriocheir sinensis. The storage device is used for solving the problem in the prior art that the eriocheir sinensis easily fights with one another in the transportation process. The storage device for preventing fighting of the eriocheir sinensis comprises storage boxes and oxygen supplying humidifiers, the storage boxes compriseinner net cylinders, outer net cylinders, bottom plates, partition plates with several box rings and several connection plates; the oxygen supplying humidifiers comprise cover plates, connection pipes, conveying pipes and several spraying pipes, and the connection pipes are connected to the cover plates; the connection pipes are vertically arranged and located under the cover plates, and the conveying pipes are vertically arranged and communicated with the connection pipes. The storage device has the advantages of being capable of effectively isolating the eriocheir sinensis and environmentally friendly, reducing cost and being convenient to clean.

The invention relates to a method for improving the quality of water in an eriocheir sinensis aquaculture pond and preventing crab diseases through mint planting and belongs to the technical field of aquaculture. Mint and eriocheir sinensis are obtained through eriocheir sinensis breeding, mint planting, pond management and harvesting. By the adoption of the method, more varieties of floating floor plants can be obtained to realize a water purifying effect same as that generated by other floating floor plants, the utilization value of floating floor plants can also be increased, and therefore application and popularization of bio-floating bed technique are benefited; furthermore, by planting mint in aquaculture water, growth of bred animals can be promoted and diseases of bred animals can be controlled through metabolites generated during growth of Chinese herbal medicine.