SNP mark related to weight of eriocheir sinensis and application of SNP mark

A technology of Chinese mitten crab, body weight, applied in the field of molecular biology

Active Publication Date: 2015-10-28
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In previous studies, many researchers have tried to explore the cultivation mode of large-scale crab species from various aspects such as the shape (area) of the crab species breeding pool, the density of

Method used

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  • SNP mark related to weight of eriocheir sinensis and application of SNP mark
  • SNP mark related to weight of eriocheir sinensis and application of SNP mark
  • SNP mark related to weight of eriocheir sinensis and application of SNP mark

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Embodiment 1

[0024] Collect more than 300 crab species of different specifications under the same breeding conditions, and measure the external morphological indicators of each crab species. The average weight is 8.26±3.29g. 170 crab species were randomly selected from them, their leg muscles were taken, their genomic DNA was extracted by phenol-chloroform method, and PCR-based restriction fragment length polymorphism (PCR-RFLP) was used to genotype all crab species. .

[0025] ①PCR reaction

[0026] The PCR product amplification length is 224bp, and the PCR primers are:

[0027] Upstream primer: 5'-GCTTCAACAGGGGAACAGTC-3' (SEQ ID NO. 2);

[0028] Downstream primer: 5'-TTAGTTTGAGGACGCACGTG-3' (SEQ ID NO. 3).

[0029] The PCR amplified sequence information is shown in SEQ ID NO. 1, in which there is a T / C polymorphism at position 126.

[0030] The PCR reaction system is as follows: 10×PCR Buffer 6.25μL, Mg 2+ (2.5mmol / L) 6.25μL, dNTP (2.5mmol / L) 3.75μL, forward and reverse primers each 1μL (10nmol / L...

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Abstract

The invention discloses an SNP mark related to the weight of eriocheir sinensis and application of the SNP mark. The SNP mark related to the weight of the eriocheir sinensis is located on the5'- side wing area of an MIH gene, the specific position is the 372bp part of the MIH gene, and nucleotide variation information is SNP g.372T>C. Obvious relevance exists between the SNP position point and the weight trait of the eriocheir sinensis species, and the average weight of the CC gene type individual is obviously higher than the average weight of the TT gene type individual. SNP g.372T>C genetic typing is carried out on a river crab breeding colony and morphology characteristic analysis is combined so that the individual with the gene type of CC can be preferably selected as a breeding parent, and the research has the significant guiding meaning on breeding of a novel large-specification river crab variety.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and relates to a SNP marker related to the weight of the Chinese mitten crab and its application. Background technique [0002] Eriocheirsisensis, also known as river crab, is an important aquaculture target in the middle and lower reaches of the Yangtze River. Under natural conditions, river crabs are generally two-year-old. Each autumn, adult crabs migrate to the estuary to mate and lay eggs. They hatch from March to May in the next year and develop into juvenile crabs (big-eyed larvae, young crabs, crab species). Going back up the river, it continues to develop and grow into adult crabs in fresh water. Generally speaking, raising crab species of larger size (body weight) during the breeding process of river crab can obtain larger size of adult crab fishing. Therefore, in the breeding process of river crab seed, the market demand for large-sized crabs has been large, the breeding efficiency is usu...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 许志强陆全平董建昌李旭光邓燕飞徐宇葛家春潘建林
Owner FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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