102 results about "Chinese mitten crab" patented technology

The invention relates to the technical field of aquaculture feedstuff. Premix material consists of composite vitamin and composite microelement according to the mass ratio of 5 to 10:15 to 20, wherein the composite vitamin consists of vitamin A, vitamin D, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, calcium pantothenate, folic acid, biotin, inositol, vitamin C, beta-glucan, garlicin, DMPT, astaxanthin, topaz, antioxidant, mildew inhibitor and wheat middlings; and the composite microelement consists of ferric citrate, zinc sulfate, magnesium sulfate, methionine, potassium iodate, manganese sulfate, copper sulfate, sodium selenite, potassium chloride, choline chloride, betaine and zeolite. The invention has stable raw material sources and complete nutrition. A cultivated object grows rapidly with a high feedstuff conversion rate and good cultivating benefits. Compared with crabs eating other feedstuff sources such as rough fish, corn, bean pulp, and the like, the quality of Chinese mitten crab is improved. The invention relates to the feedstuff additive premix material for the Chinese mitten crab.

The invention discloses a technology for ecological breeding of large Chinese mitten crabs (female crabs 150g and male crabs more than 200g) in a pond. The method comprises the steps of (1) selection and transportation of the large parent crabs, (2) mating of the parent crabs, (3) over-wintering fatting and management of the parent crabs, (4) catching and temporary breeding of egg-carrying crabs, (5) cage hanging and distribution of the egg-carrying crabs, (6) food organism culture, (7) zoea cultivation, (8) megalopa desalting. The technology for ecological breeding of the large Chinese mitten crabs in the pond is developed for emphasizing to improve the over-wintering survival rate and the egg-carrying rate of the large parent crabs and the survival rate of the egg-carrying crabs according to features of reproductive biology and reproductive ecology of the Chinese mitten crabs on a basis of in-pond ecological breeding work of large parent crabs. Technical practices of an inventor for nearly a year proves that unprecedented success on the in-pond ecological breeding of the large parent crabs is achieved, an in-pond ecological breeding technical system of the large parent crabs is established, and profound influence is made in the Chinese mitten crab breeding industry of our country.

The invention relates to the water quality purification field, and especially relates to a method for purifying water quality; the method achieves a purpose of purifying the water quality by pond culture of Chinese mitten crab. The method for purifying the water quality includes the following steps: desilting and tidying a pond, namely carrying out desilting treatment on excessive silt in the crab pond; clearing and drying the pond, namely mixing quick lime, a bleaching powder and tea meal and clearing the pond; putting aristichthys nobilis and a first generation of freshwater shrimps in the middle ten days of February; putting crab seedlings in the first ten days of March; putting margarya melanoides before and after Tomb-sweeping Day in batches; harvesting the freshwater shrimps with a ground bamboo cage in the middle ten days and the last ten days of June; putting a second generation of freshwater shrimps in the first ten days of July; harvesting the crabs, the freshwater shrimps and the aristichthys nobilis from October to November; and during the culture process, carrying out fertilization water treatment and planting waterweeds in the crab pond. Through the intercropping breeding mode, aquatic products are cultured while the water quality is purified, and the obtained cultured river crabs have good quality.

The invention discloses a method for identifying the quality of Chinese mitten crabs based on a hyperspectral image technology. The method comprises: 1, acquiring a Chinese mitten crab sample comprising a plurality of female Chinese mitten crabs and a plurality of male Chinese mitten crabs, 2, identifying the quality of the Chinese mitten crabs according to the Chinese mitten crab sensory indexesof the agricultural standard NY-5064-2001, 3, acquiring and correcting hyperspectral images, 4, acquiring spectral information of the region of interest, 5, extracting abdominal spectral feature variables and back image feature variables, carrying out PCA analysis on the obtained crab shell hyperspectral images and abdominal surface spectral information to obtain first 3 principal components suchas back image texture feature information and abdominal spectrum information capable of characterizing the original information of the sample and 6, constructing an identification model which is a LS-SVM model and identifying the quality of Chinese mitten crabs through the identification model. The method overcomes the shortcomings of slow manual identification and subjective influence, and improves the intelligent detection level of Chinese mitten crabs.

The invention discloses an aquaculture wireless sensor network node device and an operation method, and belongs to the technical field of water quality monitoring. The aquaculture wireless sensor network node device and the operation method are suitable for many application occasions such as water quality parameter online detection in aquaculture of Chinese mitten crabs, fish, shrimps, shellfish and the like and used for collecting parameter data of dissolved oxygen content (mg/L) and water temperature ( DEG C) in an aquaculture pond in real time online and sending the data to a wireless sensor network gateway or a wireless sensor network base station and a local terminal in a 433MHz frequency band wireless sensor network communication manner after further changing, possessing and storing the parameter data.

The invention relates to a Chinese mitten crab breeding method. The method comprises the steps that Chinese mitten crabs are selected as parent crabs, and male parent crabs and female parent crabs are separated for temporary breeding before being subjected to mating; egg-carrying crabs are bred two months or above at the water temperature below 5 DEG C; the egg-carrying crabs lay eggs which grow into zoeas and big-eye larvae; crablets grow into juvenile crabs, seed crabs and mature crabs. In the process of growing from big-eye larvae to crablets and juvenile crabs, water quality in a culture pond is supposed to comply with regulations in Water Quality for Pollution-free Food Freshwater Aquaculture; the crablets at the stage I are later fed with fresh colorful millet together with coagulated pig blood and minced bean curd; animal bait takes up 70% or above before the seventh month and after the ninth month of the culture stage of the juvenile crabs, and 90% or above between the seventh month and the ninth month. Compared with existing breeding methods, the method has the advantages that the crabs grow quickly and are high in disease resistance; the weight of all crabs is increased by 10.2% compared with that obtained through the existing breeding methods; the disease occurrence rate is reduced from 8%-10% to 3% or below.

The invention discloses a Chinese mitten crab freshness damage-free detection method based on semi-supervised identification projection. The detection method includes: pre-processing a sample and pre-heating an electronic nose apparatus; sampling the sample by deeply putting a sample needle of the electronic nose apparatus into a beaker; collecting smell data collected by a sensor array in the electronic nose apparatus, pre-processing the data, and performing character recognition process to the pre-processed sensor original data. In the method, low-dimension feature vectors extracted through the semi-supervised identification projection algorithm serves as an input mode vector of a self-organizing feature mapping neural network, thereby forming a prediction model for level of freshness of the Chinese mitten crabs. The method is used for damage-free detection of the sample and is greatly improved in detection precision, and can achieve quick and damage-free detection on the quality of the Chinese mitten crabs.

The invention relates to the field of genetic thremmatology of aquatic products, in particular to a multielement high flux genetic marking system and a genetic analyzing method of Chinese mitten crabs. The genetic marking system is a mitochondria sequence information system and a microsatellite molecule marking system, wherein the microsatellite molecule marking system comprises 7 EST-SSR systems marked 1and 6 EST-SSR marked systems 2. The method comprises the following steps: later generations produced by the same parent and different parents in a polyculture family are separated according to hereditary constitution and genetic diversity through information of sequences of three pairs of primer amplifications COI, Cytb and CR of mitochondria, and male parent discrimination is performed on later generations of the discriminated parents according to genetic distance and genetic diversity through the EST-SSR microsatellite molecule marking system, thereby completing the genealogy authentication during the genetic breeding of the Chinese mitten crabs and determining the inbreeding coefficients. The invention discriminates the genetic relationship among filial generations by using the multielement high flux genetic marking system on the basis of effectively utilizing species genetic information, and effectively avoids the inbreeding recession.

The invention relates to a method for discriminating Chinese mitten crabs in different producing areas, which is characterized by comprising the following steps of: fishing for the Chinese mitten crabs in different producing areas and then placing in a refrigerator for preservation; after thawing the crabs, taking the third ambulatory legs at the left sides of crab carapaces for element analysis,and then drying the third ambulatory legs in a drying oven to the constant weights; grinding dry samples by using agate mortars for homogenization, and then placing in the drying oven at room temperature; weighing and placing the dry samples into a digestion pipe, and adding concentrated nitric acid to the digestion pipe for digesting the dry samples; transferring the digested samples into a quantitating bottle, and then determining the volume of the samples by using pure water to obtain a solution to be measured; measuring the concentration values of trace elements in the solution to be measured and calculating the corresponding concentration values in the third ambulatory leg dry samples of the Chinese mitten crabs. The analysis method in the invention has reliability and favorable precision, and can effectively discriminate Chinese mitten crab crowds respectively produced in the Jiangsu Yangcheng Lake, the Yong Lake, the Changdang Lake and the Jiangxi Junshan Lake and establish a specific trace element geographical fingerprint library and a discrimination function equation of the Chinese mitten crabs in various areas.

The invention discloses a pond crab strengthened breeding method. The method includes a, pond improvement and construction; b, preparatory work prior to stocking; c, juvenile crab placement; d, breeding management. Compared with the prior art, the method has the advantages that the juvenile crabs are bred in concentrated and strengthened manners, the physique is strengthened, and the survive rate after stocking is increased; the juvenile crabs are placed into a commercial crab breeding pond in the middle third of the May, a traditional placement time is avoided, the time for planting water plants in the pond is sufficient before the juvenile crabs are placed into the pond for breeding, and fine environment for middle and rear periodical molting and growth is provided for Chinese mitten crabs; the quality and quantity of the juvenile crabs stocked in the pond in May are grasped clearly, and the blindness of feed placement is avoided.

The invention relates to an ecological aquaculture method of high quality Chinese mitten crabs and belongs to the technical field of aquaculture. The method comprises the steps that a pond is selected, cleaned, prepared and sterilized; from February to March in early spring, aquatic plants are planted, a '2+2' composite aquatic plant planting mode is adopted for aquatic plant planting, waterweed and potamogeton maackianus are used as main plants, and wild celery and hydrilla varticillata are used as auxiliary plants; young crabs are put; when the water temperature of the pond reaches above 10DEG C, feed starts to be put; from May to June, siniperca chuatsi and incubation freshwater shrimps are put into the pond; before the Qingming festival, snails start to be put into the pond; during daily management, the change of the water quality is observed, the water quality is adjusted, facilities are overhauled, and pests are removed in good time. According to the method, the aquaculture yield and specification of adult crabs are decided through the reasonable aquaculture density and breeding specification. 800 crabs are bred per mu, about 600 adult crabs are harvested per mu, the large-scale and high-quality brand crabs are bred through a '863' mode with output value of 30,000 yuan pre mu, and the method is an ecological aquaculture mode worthy of copying and popularization.

The invention relates to a sex development regulation and control technology for the male Chinese mitten crab, in particular to a sex reversal siRNA of the Chinese mitten crab and application of the siRNA. A nucleotide sequence of a positive-sense strand of the sex reversal siRNA of the male Chinese mitten crab is shown in SEQ ID NO.1, and a nucleotide sequence of an antisense strand is shown in SEQ ID NO.2. according to the sex reversal siRNA, an IAG gene segment of the Chinese mitten crab is screened from an androgenic gland transcriptome of the Chinese mitten crab, the total-length cDNA sequence of the segment is cloned, according to an IAG gene sequence of the Chinese mitten crab, the siRNA is designed, synthesized and guided into a body of the Chinese mitten crab in the sex differentiation period, expression of an IAG gene in an androgenic gland cell is disturbed, and sex reversal of the male crab is achieved. Compared with androgenic gland removal through surgery, a micro-injection method is adopted and has the advantages that the trauma to the body of the Chinese mitten crab is small, and the situation is avoided that due to wound infection after surgery, the Chinese mittencrab dies; the injection dose is extremely low, the immunological stress reaction of a tested animal is not easily triggered, and accordingly the success rate of sex reversal is increased.

The invention belongs to the technical field of nondestructive testing, and relates to a determining method for quality grade of Chinese mitten crab based on information fusion. The method comprises the following steps: collecting a hyperspectral image of visible near infrared band of the crab shell of Chinese mitten crab and conducting a correction; then dividing the region of interest, extracting RGB information, also extracting the spectral information of visible near infrared band in the region of interest and screening characteristic wavelength; filtering processing the corrected hyperspectral image in step 1 and extracting texture information of the region of interest after obtaining a principal component image; and finally fusing the characteristic wavelength, the texture information and the RGB information and through an algorithm establishing a hierarchical model based on fusion information. Information fusion technology is utilized, the internal and external attribute information of Chinese mitten crab are combined, and the quality grades of Chinese mitten crab are embodied more three-dimensionally and comprehensively. The determining method has the advantages that the accuracy of classification is high, and the performance is more stable; and the defects of the prior art with single index, complicated process and low efficiency are solved.

The invention relates to a quantitative determination method for migration speed and a course of Yangtze River Chinese mitten crab parent crabs. The number of sifted parent crabs is 5000-10000. The quantitative determination method for the migration speed and the course of the Yangtze River Chinese mitten crab parent crabs is characterized in that marks are coated on the parent crabs and lantern ring symbols are tied to the parent crabs. On the same day, the parent crabs are released in the river in the region which is overlapped with a migration locus of natural population parent crabs, and the longitude and the latitude of the release point and the time of release are recorded. Three monitoring sections of fixation gill nets are set in the lower course, 4-8 monitoring points are arranged on each monitoring section, the caught marked parent crabs by each monitoring point are checked each day, and the time and the quantity of catching are recorded. From the time that the first marked parent crab is caught, seven times are counted continuously, the migration speed V=S (T1-T2), S is the distance between the release point and the monitoring points, T1 is the time of catching the parent crabs, and T2 is the time of release. The fastest migration speed and the average migration speed are calculated, the quantity of the caught parent crabs of each monitoring point on the three monitoring sections is counted, and the release locus is connected with the monitoring points of catching the parent crabs and the migration course of the parent crabs and the proportion of the parent crabs are obtained.

The present invention discloses a Chinese mitten crab fattening feed which comprises the following components in percentages by weight: 25% of fish meal, 35% of soybean meal, 5% of cottonseed meal, 21% of corn starch, 1.4% fish oil, 2.4% of soybean oil, 0.5% of soybean lecithin, 0.5% of cholesterol, 3% of multi-vitamins, 2% of multi-minerals, 0.5% of choline chloride, 2% of adhesion agents and 1.7% of bran. The invention also discloses a compound Chinese mitten crab fattening feed which includes the above-mentioned Chinese mitten crab fattening feed and astaxanthin. Compared with the prior art, the fattening feed reduces the application amount of fish meal, the obtained Chinese mitten crabs fed by the fattening feed are high in weight gain, and steam-cooked Chinese mitten crabs are better in color and luster, redder in crab sperms, and higher in nutritional ingredients of edible parts.

The method of temporarily keeping river crab for sale includes four parts, including selecting temporarily keeping pond, selecting river crab to be kept, determining temporarily keeping density and managing. It features the selection of temporarily keeping pond near water source with convenient water pouring and draining ports, area of 3-5 unit of area and water depth of 1.5-1.8 m; selecting robust river crab and sterilizing with 4 wt% concentration salt solution for 10-15 min; the temporarily keeping density of 2000-2500/mou or 200-250 kg/unit of area and temporarily keeping period of 1-3 months; and managing including laying bait of 3-5 wt% of crab weight once a day, changing water of 15-25 cm once every 5-7 days, spraying lime once every 20-30 days, spraying 0.5 ppm concentration trichloroiso cyanuric acid concentration to sterilize water regularly, and feeding terramycin and furazolidone mixed into feed for continuous 5 days every half one month.

The invention relates to an imitation ecological cultivating method for intercropping the Chinese mitten crab with the all-male yellow catfish, which includes the steps: selecting and sterilizing a fishpond, throwing in catfish fry and crab seeds, feeding the fish fry and the crab seeds, and regulating water quality. The imitation ecological cultivating method has the advantages that water productivity is expanded, growth advantage of the all-male yellow catfish is given to full play in the method, water spatial ecological advantages are fully utilized, productive potential of the fishpond is tapped further, utilization coefficient of pond water is improved, economic benefit is improved, yield and production value of the all-male yellow catfish in intercropping are improved, and comparative benefit of the Chinese mitten crab in pond culture is improved.

The present invention belongs to the technical field of cell culture and particularly relates to a Chinese mitten crab blood cell culture medium and a culturing method. The Chinese mitten crab blood cell culture medium comprises a basic culture medium and added components, wherein the basic culture medium is selected from a TC-100 basic culture medium; and the added components and concentrations thereof are as follows: 1%-10% of a buffer solution, 1g/L-2g/L of calcium chloride, 1g/L-2g/L of alkali metal chloride, 1.5g/L-2.5g/L of glucose and 1%-1.5% of antibiotic. The culture medium significantly improves survival rate of Chinese mitten crab blood cells in in-vitro culture and prolongs duration of the blood cell culture in vitro, and the blood cells cultured in vitro can maintain good cellmorphology.

The invention discloses a Chinese mitten crab uniqueness recognition method based on image matching, and belongs to the field of digital image processing. The method comprises the following steps of 1, acquiring the original image A of a Chinese mitten crab, and then segmenting the back image M of the Chinese mitten crab; 2, extracting the feature points of the back image M of the Chinese mitten crab; 3, extracting, from a database, a saved back image Q and feature points of the Chinese mitten crab, matching the feature points of the image Q and the image M; 4, detecting mismatched feature points; 5, calculating the similarity of the image Q and the image M; and 6, ending the matching and outputting crab matching information. The object of the invention is to overcome the problem that theuniqueness of the Chinese mitten crab cannot be effectively recognized in an existing detection method, and determine the uniqueness of the Chinese mitten crab based on similarity by analyzing the images of various recesses, protrusions and textures widely distributed on the crab carapace, and then comparing the images with images saved in a database.

The invention provides a water environment evaluation method for the culture of Chinese mitten crabs. According to the invention, the culture cycle of Chinese mitten crabs is divided into a pluralityof culture stages according to the shell shedding cycle of Chinese mitten crabs. The method comprises the steps of S1, acquiring water environment parameters for the culture of Chinese mitten crabs during any culture stage, and obtaining water environment features according to the change of water environment parameters over the time and the relationship between water environment parameters and a water environment threshold value; S2, inputting feature vectors into a staged water environment classification model, and obtaining the valuation result of the water environment for the culture of Chinese mitten crabs. According to the invention, corresponding water environment features are obtained from the water environment parameters acquired during any culture stage. Meanwhile, water environment features are adopted as feature vectors to be inputted into the staged water environment classification model. In this way, the evaluation result of the water environment during the culture stagesof Chinese mitten crabs can be obtained. Moreover, according to the evaluation result of the water environment, the water environment is correspondingly adjusted. As a result, farmers for the cultureof Chinese mitten crabs can be assisted to make production decisions.

The invention discloses engineered saccharomyces cerevisiae capable of efficiently expressing Chinese mitten crab chitin and belongs to the biotechnology field. According to the engineered saccharomyces cerevisiae disclosed by the invention, cDNA of a Chinese mitten crab anti-lipopolysaccharide factor EsCrustin is cloned to a vector plasmid pHAC181, a successfully cloned positive transformant is subjected to sequencing verification, then a homologous recombination method is utilized for integrating a target gene to downstream of a GAL1 promoter in a saccharomyces cerevisiae strain, and under the induction action of galactose, the target protein EsCrustin is efficiently expressed.

The invention discloses an anti-stress immunopotentiator for a Chinese mitten crab juvenile crab and a preparation method and application thereof. The method is as follows: taking fructooligosaccharides, lactic acid bacteria, Bacillus subtilis and yeast; respectively crushing the fructooligosaccharides, the lactic acid bacteria, the Bacillus subtilis and the yeast with a superfine crusher, and then sieving with a 120 mesh screen; evenly fully mixing the lactic acid bacteria, the Bacillus subtilis and the yeast in a mixer according to the proportion, and mixing the fructooligosaccharides with the lactic acid bacteria, Bacillus subtilis and yeast mixture evenly to obtain the powdery anti-stress immunopotentiator. The anti-stress immunopotentiator has the advantages of stable sources of raw materials, wide range of raw materials, simple preparation and high cost performance; the anti-stress immunopotentiator is based on the immune mechanism of crustaceans, and is helpful to improve the antioxidant capacity of crab hepatopancreas, remove free radicals in body, improve immunity and anti stress ability of the body, and promote the healthy growth of the juvenile crab when in use.

The present invention relates to a seasoning material and a preparation method thereof, and particularly relates to a seasoning material for Chinese mitten crabs and a preparation method thereof. Theseasoning material comprises 5-7 parts of edible vinegar, 0.2-1 part of soy sauce, 0.8-1.5 parts of water, 0.5-1 part of ginger, 0.2-1 part of rock sugar, 0.01-0.03 part of sesame oil, 0.01-0.03 partof shallot, 0-0.01 part of purple perilla, 0-0.02 part of wine, 0-0.02 part of garlic and 0.5-2 parts of coconut oil. Traditional fermented products, vegetables and other raw materials are used, a Chinese cooking technique is used, and the seasoning material is finely prepared and free of any additives, and can be naturally blended with the delicious taste of the Chinese mitten crabs.

The invention relates to a novel method for inducing Chinese mitten crab triploids. The method includes the following steps of seawater domestication of parent crabs, marking of the parent crabs, determination of a drug-induced start moment based on morphological markers, determination and application of KCI inducing optimal parameters and taking of drug permeability increasing measures. By the adoption of the steps of limitation of seawater domestication time, marking of the parent crabs, determination of the drug-induced start moment based on the morphological markers, taking of the drug permeability increasing measures and the like, stability and effectiveness of inducing the Chinese mitten crab triploids are obviously improved, and a technical support is provided for Chinese mitten crab triploid seed large-scale cultivation.