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Method for discriminating Chinese mitten crabs in different producing areas

A technology of Eriocheir sinensis and origin, applied in the preparation of test samples, measuring devices, and material analysis through electromagnetic means, can solve the problems of unobvious and indistinguishable genetic polymorphism sites, and achieve reliable Strong discriminative, good precision, and reliable analysis methods

Inactive Publication Date: 2012-12-12
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The genetic polymorphism sites of Chinese mitten crabs in different water systems are not obvious, so they cannot be distinguished

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment one: a kind of method of the present invention distinguishes Chinese mitten crab from different places of origin, adopts following process steps:

[0024] Eriocheir sinensis samples (6 each) from Yangcheng Lake and Ge Lake were collected in December 2008, and were stored at 0-4°C.

[0025] The sample first measures biological data such as body length and weight. Then store in -20°C refrigerator for use. After thawing, take the third step and rinse 6 times with ultrapure water (Milliprore-Simplicity Personal Ultrapure Water System, Milliprore-Simplicity, USA, effluent resistivity 18.2MΩ.cm), and dry in a drying oven at 80°C for 24h to constant Heavy; in the third step, the dry sample is ground into a powder and homogenized (that is, a powdery dry sample is made), and placed in a desiccator (at room temperature) for use (it can be used immediately).

[0026] Accurately weigh 0.1g of powdered each mitten crab third step foot dry sample and pack into Teflon (Te...

Embodiment 2

[0029] Embodiment two: a kind of method of the present invention distinguishes Chinese mitten crab from different places of origin, adopts following process steps:

[0030] Eriocheir sinensis samples (6 each) from Yangcheng Lake and Changdang Lake were collected in December 2008, and were stored at 0-4°C.

[0031] The sample first measures biological data such as body length and weight. Then store in -18°C refrigerator for use. After thawing, take the third step and rinse 7 times with ultrapure water (Milliprore-Simplicity Personal Ultrapure Water System, Milliprore-Simplicity, USA, outlet water resistivity 18.2MΩ.cm), and dry in a drying oven at 90°C for 25h to constant Heavy; in the third step, the dry sample is ground into a powder and homogenized (that is, a powdery dry sample is made), and placed in a desiccator (at room temperature) for use (it can be used immediately).

[0032] Finely weigh 0.15g of each mitten crab third step dry sample in powder form and pack into a...

Embodiment 3

[0035] Embodiment three: a kind of method of the present invention distinguishes Chinese mitten crab from different places of origin, adopts following process steps:

[0036] The Chinese mitten crab samples (6) from Yangcheng Lake were collected in December 2008, and the Chinese mitten crab samples (6) from Junshan Lake in Jiangxi Province were collected from November 2009, both at 0-4°C save.

[0037] The sample first measures biological data such as body length and weight. Then store in -18°C refrigerator for use. After thawing, take the third step and rinse 8 times with ultrapure water (Milliprore-Simplicity Personal Ultrapure Water System, Milliprore-Simplicity, USA, outlet water resistivity 18.2MΩ.cm), and dry in a drying oven at 100°C for 26h to constant Heavy; in the third step, the dry sample is ground into a powder and homogenized (that is, a powdery dry sample is made), and placed in a desiccator (at room temperature) for use (it can be used immediately).

[0038]...

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Abstract

The invention relates to a method for discriminating Chinese mitten crabs in different producing areas, which is characterized by comprising the following steps of: fishing for the Chinese mitten crabs in different producing areas and then placing in a refrigerator for preservation; after thawing the crabs, taking the third ambulatory legs at the left sides of crab carapaces for element analysis,and then drying the third ambulatory legs in a drying oven to the constant weights; grinding dry samples by using agate mortars for homogenization, and then placing in the drying oven at room temperature; weighing and placing the dry samples into a digestion pipe, and adding concentrated nitric acid to the digestion pipe for digesting the dry samples; transferring the digested samples into a quantitating bottle, and then determining the volume of the samples by using pure water to obtain a solution to be measured; measuring the concentration values of trace elements in the solution to be measured and calculating the corresponding concentration values in the third ambulatory leg dry samples of the Chinese mitten crabs. The analysis method in the invention has reliability and favorable precision, and can effectively discriminate Chinese mitten crab crowds respectively produced in the Jiangsu Yangcheng Lake, the Yong Lake, the Changdang Lake and the Jiangxi Junshan Lake and establish a specific trace element geographical fingerprint library and a discrimination function equation of the Chinese mitten crabs in various areas.

Description

technical field [0001] The invention relates to a method for discriminating various trace elements in Chinese mitten crabs from different origins, which can systematically and quickly establish the accumulation characteristics and geographical environment characteristics of various trace elements in Chinese mitten crabs from different water areas; The characteristics can more effectively and quickly identify content such as resource groups of Chinese mitten crabs from different origins, and belong to the technical field of biological monitoring environment. Background technique [0002] "Origin" means that a commodity originates from a certain region, and the specific quality, reputation or other characteristics of the commodity are mainly determined by the natural or human factors of the region. For aquatic products, the geographical environment and natural environmental conditions of a water area inevitably determine the unique quality and taste of the aquatic products pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64G01N1/28
Inventor 杨健刘洪波徐跑
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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