The invention discloses a plant antifreezing agent for enhancing enzyme activity and a preparation method thereof. The plant antifreezing agent is characterized in that it is prepared from the following raw materials by weight: 1-2 parts of ethephon, 6-12 parts of polylactic acid, 1-2 parts of paclobutrazol, 3-6 parts of sorbitan fatty acid ester, 8-15 parts of chloroform, 25-35 parts of water, 6-12 parts of corn protein powder, 2-3 parts of calcium chloride, 1-3 parts of glucose, 2-4 parts of amino acid, 4-6 parts of glycerin, 1-2 parts of sodium dodecyl sulfate, 2-5 parts of magnesium chloride, and 2-5 parts of urea. According to the invention, corn protein powder is soaked in glucose and amino acid, and is adhered to a plant through the bonding effect of polylactic acid, thus achieving a heat preservation effect, also the glucose and amino acid therein can significantly increase the content of sugar and free amino acids in plants, have a protective effect on cells, and effectively reduce the influence of freezing damage to plants. Through cooperation with the added ethephon and the like, the activity of enzyme in the cells can be promoted, and heat is generated.