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Endometrium stem cell serum-free medium

A technology of serum-free medium and endometrial stem cells, which is applied in the cultivation process, animal cells, tissue culture, etc., can solve the problems of low purity and many components of the medium, and achieve high purity, low cost, and less miscellaneous cells Effect

Inactive Publication Date: 2017-05-24
GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under the conditions of the existing culture technology, endometrial stem cells use a lot of medium components, and the purity is not high

Method used

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  • Endometrium stem cell serum-free medium

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Embodiment 1

[0051] A serum-free culture medium for endometrial stem cells in this embodiment comprises the following components in terms of total volume percentage:

[0052] Low sugar DMEM medium 70-85%

[0053] Serum Replacement 5-10%

[0054] Vitamin C 2-10%

[0055] L-Glutamine 1-5%

[0056] Glucose 1.25-15%

[0057] 2-Mercaptoethanol 1-10%

[0058] Penicillin and streptomycin double antibody solution 0.8-1.5%

[0059] Gentamicin sulfate 0.8-1.5%.

Embodiment 2

[0061] A serum-free culture medium for endometrial stem cells in this embodiment comprises the following components in terms of total volume percentage:

[0062] Low sugar DMEM medium 70%

[0063] Serum Replacement 5%

[0064] Vitamin C 2%

[0065] L-Glutamine 1%

[0066] Glucose 1.25%

[0067] 2-Mercaptoethanol 1%

[0068] Penicillin and streptomycin double antibody solution 0.8%

[0069] Gentamicin Sulfate 0.8%.

Embodiment 3

[0071] A serum-free culture medium for endometrial stem cells in this embodiment comprises the following components in terms of total volume percentage:

[0072] Low sugar DMEM medium 80%

[0073] Serum Replacement 7%

[0074] Vitamin C 6%

[0075] L-Glutamine 3%

[0076] Glucose 5%

[0077] 2-Mercaptoethanol 6%

[0078] Penicillin and streptomycin double antibody solution 1%

[0079] Gentamicin Sulfate 1%.

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Abstract

The invention relates to an endometrium stem cell serum-free medium, which is prepared from the following components in percentage by total volume: 70 to 85 percent of low-sugar DMEM (Dulbecco Modified Eagle Medium), 5 to 10 percent of serum replacement, 2 to 10 percent of vitamin C, 1 to 5 percent of L-glutamine, 1.25 to 15 percent of glucose, 1 to 10 percent of 2-mercaptoethanol, 0.8 to 1.5 percent of mycillin double-antibody solution, and 0.8 to 1.5 percent of gentamicin sulphate. The endometrium stem cell serum-free medium provided by the invention is adopted for carrying out cell culture, and has the characteristics of less medium component, stable endometrium stem cell growth, high multiplication capacity and high purity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a serum-free culture medium for endometrial stem cells. Background technique [0002] Stem cells are a kind of primitive cells with self-renewal ability and multi-lineage differentiation potential under suitable microenvironment. Endometrial stem cells are pluripotent stem cells, which have the characteristics of strong proliferative ability, no chromosomal mutation, and no tumorigenesis. Compared with other stem cells, endometrial stem cells have obvious characteristics: there are many such stem cells, and they are bone marrow stem cells. 30 times that of stem cells. Endometrial stem cells were first extracted from curettage tissue. With the deepening of research, people found that stem cells with regenerative ability can also be isolated from women's menstrual blood, and the acquisition method is non-invasive and has no ethical issues. The method is simpler and more convenient. S...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0682C12N2500/32C12N2500/34C12N2500/38C12N2500/44C12N2500/90
Inventor 李相鲁张严冬谢海涛聂广军
Owner GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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