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Human stem cell RZSXF-SC and establishing method and application thereof

A technology of RZSXF-SC and establishment methods, applied in the field of stem cells, can solve problems such as cell therapy that is difficult to meet, achieve good skin tissue repair ability, improve low cell viability, and maintain stable stemness

Inactive Publication Date: 2020-05-15
THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Human bone marrow mesenchymal stem cells are relatively abundant, but only account for 0.001%-0.01%, which is difficult to meet the needs of cell therapy, so it needs to be separated and purified in vitro, cultured and expanded to meet the requirements
Moreover, the current mesenchymal stem cells still have cell viability, proliferation ability, self-renewal ability, and cell stemness maintenance that need to be further improved.

Method used

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  • Human stem cell RZSXF-SC and establishing method and application thereof
  • Human stem cell RZSXF-SC and establishing method and application thereof
  • Human stem cell RZSXF-SC and establishing method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0096] Example 1 Isolation and cultivation of human stem cells

[0097] Aseptically collect human tissue umbilical cord, remove 2 arteries and 1 vein and cut into 2-3mm 3 About the size, spread evenly in the petri dish and let it stick to the wall for 2-4 hours. The serum-free system requires fibronectin (Corning, 356008) to coat the culture dish. After adding the medium to the culture dish, add fresh medium every 2-3 days, and continue to detect. When the cell saturation reaches about 80%, the cells are collected.

[0098] The culture system used includes DMEM / F12 (Corning), 10% FBS (Gibco) system, RZSXF medium, StemPro (Gibco, A10332-01) system, MesenCult (STEMCELL Technologies, 05448) system, NutriStem (Biologcial Industries, 05-200 -1A, 05-201-1U) system, HY (Shanghai Hengyuan Biology, CCGmHuM) system.

[0099] UCMSCs were counted using Rigel (CountStar), and cell viability was detected using a CCK-8 kit (4ABiotech, FXP132-500).

[0100] Among them, the RZSXF medium co...

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Abstract

The invention relates to the field of stem cells, and in particular to human stem cell RZSXF-SC and an establishing method and application thereof. The human stem cell RZSXF-SC has a deposit number ofCCTCC NO: C2019255. The RZSXF-SC is isolated from human tissue cells and is found to be capable of rapidly proliferating compared with other stem cells through experiments. The RZSXF-SC has typical MSCs surface markers and has multidirectional differentiation capability. In addition, studies prove that RZSXF-SC has stable cell stemness, and is suitable for preparing various cells required for clinical treatment. In the establishing method, primary and subculture of the RZSXF-SC is carried out by using RZSXF culture medium with clear composition without serum and animal-derived components. Theestablishing method is easy to operate, can quickly obtain a large number of target cells, and at the same time guarantees maintenance of cell stemness.

Description

technical field [0001] The present invention relates to the field of stem cells, in particular to a human stem cell RZSXF-SC and its establishment method and application. Background technique [0002] Stem cells are a class of cells with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells. Pluripotent stem cells are a type of stem cells that have the ability to produce multiple types of cells, but have lost the ability to develop into a complete individual. Mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells, which have all the common characteristics of stem cells, that is, the ability of self-renewal and multidirectional differentiation. Mesenchymal stem cells originate from the mesoderm and exist in bone marrow, umbilical cord, umbilical cord blood, fat, deciduous tooth tissue, placenta, etc., have the self-replication and multi-directional differentiation of stem cells, and can differen...

Claims

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Application Information

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IPC IPC(8): C12N5/074A61P17/02A61P37/02A61P39/06A61P29/00
CPCA61P17/02A61P29/00A61P37/02A61P39/06C12N5/0602C12N2500/10C12N2500/24C12N2500/30C12N2500/32C12N2500/38C12N2500/44C12N2500/46C12N2501/10C12N2501/115C12N2501/12C12N2501/125C12N2501/148C12N2501/23C12N2501/30C12N2501/345C12N2501/998C12N2509/00
Inventor 任浩张智勇杨逸禧叶苗漫黄龙
Owner THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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