70 results about "Gentamicin sulphate" patented technology

The invention relates to an extremely-low sperm freezing protective agent and application thereof. The protective agent is prepared from the following ingredients: sodium chloride, potassium chloride, magnesium sulfate, monopotassium phosphate, calcium chloride, fructose, trehalose, sodium citrate, glycin, glutamine, pentoxifylline, vitamin E, HAS (human serum albumin), glycerin and gentamicin sulphate. The extremely-low sperm freezing protective agent can protect less sperms with low activity and without forward activity ability in seminal fluid, can remarkably improve the activity ability of the sperms which swing weakly and cannot move forwards in seminal fluid after freezing and thawing, has no obvious influence on the physiological function of the sperms, has high freezing anabiosis rate, can be used for cryopreservation of less and weak sperms, testicle sperms and epididymis sperms, and contributes to the successful development of assisted reproductive technology.

The invention provides a blocking buffer for encapsulated plate, which consists of 0.5 to 10g/L of protein substances, 0.5 to 5g/L of sugar substances, 0.5 to 10g/L of amino acid substances, 0.2 to 2g/L of surfactant, 50 to 5 million unit/L of gentamicin sulphate, 0.2 to 2g/L of Proclin 300 and 0.02mol/L of phosphate buffer with the pH of 7.4. The encapsulated plate using the blocking buffer of the invention can not only block off biological active substances, but can also maintain the activity of the biological active substances for a longer time when the encapsulated plate departs from low temperature environment, thus the problem in relevant technology that the encapsulated plate is liable to lose activity when departing from lower temperature storage environment can be settled. In addition, the blocking buffer of the invention is easy in formulation, free from the impact on sensitivity, low in cost and simple in operation and has no environmental pollution and no toxicity and harm to human body.

The invention discloses to the method used to culture bird stem spermatogonium in vitro. It includes the following steps: inoculating the bird stem spermatogonium to CEF feeder layer; adding 10% calf blood serum, 2% chicken blood serum, 2mmol/L L-glutamine, 1mmol/L sodium pyruvate, 5.5*10-15ng/ml beta-mercaptoethanol, 10 mul/ml non-essential amino acid, 15-20ng/ml hSCF, 10U/ml mLIF, 10-15ng/ml bFGF, 0.04ng/ml hIL-11, 10ng/ml IGF, 100U/ml gentamicin sulphate culture solution into DMEM high glucose culture medium. This culturing method can process vitro operation for chicken stem spermatogonium, and lay study foundation for chicken embryo stem spermatogonium further series building.

The invention relates to a berberine hydrochloride compound preparation made from the following components in terms of weight percentage: 0.5-1% of berberine hydrochloride, 2-5% of antibacterial medicament, 0-0.1% of antiviral medicament, 1-1.5% of antibacterial synergist, 0-15% of stabilizer, 0-30% of cosolvent, 0-5% of local anodyne, 0-2% of antioxidant and balance of disperse medium. The inventive compound preparation has the following advantages: (1) the compound preparation solves the instability problem of berberine injection by making the berberine into orally taken liquid; (2) the berberine hydrochloride when used with enrofloxacin and TMP together can be used as the preferable medicament for intestinal infections, is more advantageous in treating intestinal infections caused by escherichia coli and salmonella than other single antibiotics sold in the market, especially good for drug resistant strains, is applied two times a day for three constant days, and prevention and treatment rate is up to 100%; (3) the berberine hydrochloride when used with enrofloxacin, gentamicin sulphate and TMP together is used to treat septicaemia, respiratory tract infections and intestinal infections caused by sensitive bacteria.

The invention the technical field of antibiotics fermentation, and provides a preparation method of gentamicin sulphate C1a. According to the preparation method, the output of gentamicin C1a is increased by improving the component dosage of a gentamicin fermentation culture medium. The preparation method of the gentamicin sulphate C1a is simple in production process and easy to operate and obtains the high-quality gentamicin sulphate C1a by improving the component dosage and extraction and separation method of the gentamicin sulphate fermentation culture medium.

The invention discloses a gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold and a preparation method, belonging to the technical field of high molecular materials. The preparation method comprises the following steps: dropwise adding gentamicin sulfate solution with concentration of 10-50 mg/ml into gelatin microspheres, placing overnight at the temperature of 4-25 DEG C and then performing freeze drying to obtain a gentamicin sulfate/gelatin microsphere complex; adding the gentamicin sulfate/gelatin microsphere complex into 2-10 percent by weight of silk fibroin solution, uniformly stirring and then performing freeze drying to obtain the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin porous composite scaffold, wherein the porosity of the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold is 75-95 percent, the aperture of the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold is 50-200 mum, and the gentamicin sulfate/gelatin microsphere complex-loaded silk fibroin scaffold has long-acting antibacterial and drug slow-release functions and can be applied to the fields of skin wound healing and wound dressing.

The invention discloses cornea mid-term preserving fluid and a preparation method thereof. Based on 1000mL, the mid-term preserving fluid consists of the following ingredients: 4g to 5g of minimum essential medium, 4g to 5g of tissue culture medium 199, 10g to 20g of low-molecular-weight dextranum, 20g to 30g of chondroitin sulfate, 100mg to 150mg of sodium pyruvate, 3g to 4g of HEPES, 20ml to 40ml of calf serum, 10ml to 20ml of non-essential amino acid, 1ml to 2ml of gentamicin sulphate, 100 microliters to 200 microliters of basic fibroblast growth factor and the balance of double distilled water. The cornea mid-term preserving fluid can promote the repair of damaged cells on corneal endothelium and the reconstruction of cellular morphology, can adjust the expression of different integrins on the surface of a cell membrane, can promote the adhesion growth of the cell and can stimulate the proliferation of the endothelial cells, and an effect for reducing the cell apoptosis and maintaining the morphology and activity of the cells can be realized for the mid-term preserved cornea.

The invention discloses a preparation method of gentamicin sulphate. The use levels of a spore slant culture medium, a seed culture medium and a fermenting culture medium used in the production process are improved. The method provided by the invention has the beneficial effects that impurities in a gentamicin sulphate product can be remarkably reduced so as to meet the standard of Chinese pharmacopoeia, and the production process is simple and easy to operate. Total impurities of the gentamicin sulphate product produced before improvement is greater than 10%, and sisomicin is greater than 5%, while the total impurities of the gentamicin sulphate product produced is less than 5%, sisomicin is less than 2%, and kanamycinum (micronomicin) is less than 3%.

The invention provides a solid culture medium and culture method of beauveria. The solid culture medium comprises vermiculite, wheat bran and corn flour in a mass ratio of 1:(1-1.2):(1-1.2), wherein vermiculite, wheat bran and corn flour are mixed and sterilized with steam for 40 minutes, thus obtaining the solid culture medium. The culture method comprises the steps of culturing beauveria inoculation fluid in a liquid enlarge culture medium to which gentamicin sulphate is added, then inoculating the beauveria inoculation fluid into the solid culture medium, and culturing the beauveria inoculation fluid at 25-27 DEG C for 7.5-8 days, thus harvesting beauveria. The solid culture medium has good moisture retention and breathability, can provide rich N and C source nutrients for beauveria proliferation, and is economical and accessible in raw materials. The culture method is simple to operate, is clear in flow, has low requirements for production environments and production equipment, is relatively low in production cost and relatively short in production period, dispenses with discharging the culture medium in production so as to completely eradicate the problem of pollution of mixed fungi in production, and is very suitable for small enterprises and production units to quickly produce beauveria.

The invention belongs to the field of animal pharmaceutical preparations, and relates to a compound gentamicin sulphat in-situ gel preparation for injection and a preparation method thereof. The in-situ gel preparation disclosed by the invention comprises the following components in parts by weight: 9.0 parts of gentamicin (9 million units), 6.0 parts of TMP (Trimethoprim), 15.5-30.5 parts of P407, 0.5-10.5 parts of P188, 30.0-90.5 parts of water, 0.01-10.0 parts of polymer blocker, 5.0-45.0 parts of solubilising stabilizer, 0.01-3.0 parts of preservative, 0.01-5.0 parts of antioxygen and a proper amount of pH adjustor. The in-situ gel preparation disclosed by the invention is freely moveable liquid at room temperature and forms semi-solid gel after drug delivery through intramuscular injection or subcutaneous injection; drug delivery is only carried out once during a treatment course; the in-situ gel preparation has the advantages of steady property, long drug delivery duration, exact curative effect, strong animal adaptation, no toxic and side effects and adverse reaction and the like, and is simple to operate and convenient for drug delivery; the in-situ gel preparation disclosed by the invention has broad-spectrum antibacterial activity and can be used as a medicine for preventing and treating diseases of pigs, sheep, dogs, cats, cattle and the like.

The invention discloses compound mequindox injection, and belongs to medicine preparation containing organic components, or anti-infective medicament for livestock. The injection of each 1, 000 liters is prepared by the following raw materials: 100 to 400 kilograms of sodium salicylate, 10 to 150 kilograms of mequindox, 5 to 110 kilograms of gentamicin sulphate, 1 to 20 kilograms of atropine sulfate, 1 to 20 kilograms of chlorphenamine maleate, and the balance being water for injection. The injection adopts combined compound preparation of the mequindox and the gentamicin sulphate, has efficiency and high efficiency for pig bacterial diarrhea, treats both symptoms and root causes, and relieves stress reaction of sick livestock body.

The invention provides an effective method for accurately and objectively measuring resistant starch content in rice by adding different types of antibiotics into starch hydrolyzate to discuss the effect of the antibiotics on starch hydrolysis and influence on a measuring result of the resistant starch content. By comparing the influences of the antibiotics on the starch hydrolysis effect and the resistant starch content of a detected sample, a result shows that the fermentation effect of miscellaneous bacteria in the starch hydrolysis process can be effectively suppressed by the antibiotics without affecting the activity of amylase; the most effective antibiotic is mixed antibiotic (four antibiotics, namely 0.40-0.52 mg/mL of penicillin, 15,000-17,000 UI/mL of gentamicin sulphate, 0.8-1.2 mg/mL of cefalexin and 0.85-1.30 mg/mL of erythromycin lactobionate, are mixed according to a proportion of 1.2:1:1.2:1 ); and the best dose is 35 microlitre.

The invention discloses novel composite bone cement with biological activity, raw materials comprise powder and liquid, the powder comprises the following parts in percentage by mass: 39%-84.3% of polymethyl methacrylate microspheres, 5%-15% of zirconium dioxide, 0.5%-3% of benzoyl peroxide, 0.2%-2% of gentamicin sulfate and 10%-41% of strontium-magnesium co-doped hydroxyapatite whiskers, The liquid comprises the following parts in percentage by mass: 98-99.6% of methyl methacrylate and 0.4-2% of N, N-dimethyl p-toluidine, mixing the powder and the liquid, stirring, standing, shaping and curing to obtain the novel composite bone cement. Bone-like apatite can be induced to be deposited on the surface of the bone cement in in-vitro simulated human body fluid, new bones can be promoted to grow on the interface of the bone cement and bone tissues in animal model rabbit bodies, good biological activity and osteogenesis promoting capacity are shown, and the compressive strength, bending strength and bending elastic modulus of the bone cement are all higher than those of conventional bone cement.

The invention discloses compound paracetamol injection and relates to the field of veterinary medicine preparation. The compound paracetamol injection is prepared from paracetamol, aminophylline, gentamicin sulphate, propylene glycol, alcohol, an antioxidant, EDTA (Ethylene Diamine Tetraacetic Acid) and water for injection. The invention also provides a preparation method of the compound paracetamol injection. As a novel veterinary compound preparation, the compound florfenicol injection has the characteristics of strong synergistic effect and low dosage, can effectively treat porcine hyperthermia and has the important significance for sustainable development of the animal husbandry.

The invention relates to a preparation method for compound clotrimazole, betamethasone valerate and gentamicin sulphate suspended ear drops for treating ear inflammation of dogs. According to the preparation method, the above several medicines are stably and uniformly stored in oily ear drops and not precipitated in several hours, with stable effective ingredient contents through a special process treatment. The production process disclosed by the invention is simpler and more convenient to operate, easily available in raw materials, beneficial to industrialized production, good in stability, convenient to store and transport, and remarkable in clinic treatment effect, as well has a large-scale production and clinic application advantage.

The invention relates to the technical field of microalgae, in particular to a microalgae fresh-keeping agent and a microalgae preservation method. The microalgae fresh-keeping composition comprises lysozyme, cinnamon essential oil and celastrus angulatus, and at least one auxiliary agent of sodium benzoate, sodium dehydroacetate, sodium carboxymethyl cellulose and gentamicin sulfate is further added into the composition to obtain the microalgae fresh-keeping agent. Experiments show that the microalgae fresh-keeping agent disclosed by the invention is used for preserving microalgae liquid or microalgae mud at normal temperature, the preservation time is long, the activity of microalgae is high and the content of infectious microbes is low as long as 6 months or above.

The invention provides an antibody diluent for enzyme-linked immunoassay and a preparation method of the antibody diluent, and relates to the technical field of enzyme-linked immunoassay. The antibodydiluent for enzyme-linked immunoassay comprises the following components, by weight, 80 to 90 parts of a 0.01 M PBS buffer solution, 0.5-1.5 parts of bovine serum albumin, 0.6-1.2 parts of cold fishskin gel, 0.1-0.2 parts of sodium trinitride, 0.3-0.5 parts of a Proclin 300 preservative, 0.2-0.4 parts of sodium lactate, 0.4-0.6 parts of gentamicin sulfate and 1-2 parts of an anticoagulant, wherein the 0.01 M PBS buffer solution is prepared from monopotassium phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride, tween-20 and deionized water. In the invention, preparation raw materials are reasonably used, and a system of the antibody diluent is optimized so that components of the antibody diluent of serum and plasma and peripheral blood are more reasonable, preservation time of the diluted antibody is effectively prolonged, and the antibody diluent is beneficial to medical use.

The invention discloses a method for purifying gentamicin sulphate. The method comprises the steps of: carrying out static exchange on a gentamicin fermentation broth by using 732 cation resin; washing by diluted hydrochloric acid; carrying out primary washing by dilute ammonia, primary elution by thick ammonia and primary concentration; and carrying out the technical processes such as pH adjustment, resin fixed bed dynamic adsorption, secondary washing by dilute ammonia, resolution, secondary concentration, salt conversion decoloration, and spraying to produce the gentamicin sulphate. According to the method, the quality indexes of the gentamicin sulphate bulk drug product can reach the requirements of Chinese Pharmacopoeia 2010, and the content of each related substance can conform to the quality requirements of Chinese Pharmacopoeia 2010 on bulk drugs for production of small-volume injection of gentamicin sulphate. The method has the characteristics of high yield, good quality and low cost. Meanwhile, original production equipment is combined in production, so that low investment cost is realized and the maximum economic benefit is achieved.

The invention discloses a kit and method for measuring creatinine. The kit comprises a first reagent, and the first reagent is prepared from monopotassium phosphate, dipotassium phosphate, ethylenediamine tetraacetic acid, Brij 35, Tween 80, ascorbic acid oxidase, creatinase, sarcosine oxidase, TOOS, peroxidase, potassium ferrocyanide, gentamicin sulphate and Tween 30. The method comprises the following steps: (1) the first reagent and a second reagent are dissolved according to the raw material components and content; (2) the dissolved first reagent is added into a to-be-measured sample; (3)the dissolved second reagent is added; and (4) the absorptivity of a solution under the wavelength of 546 nm is measured. According to the kit and method for measuring the creatinine, the precision and accuracy are high, and stability is achieved.

The invention relates to a method for short-period fermentation production of low-impurity gentamicin by using micromonospora purpurea. The method comprises a spore slant medium, a primary seed medium, a secondary seed medium, a tertiary seed medium and a fermentation medium. The method is cheap in fermentative raw materials, short in fermentation period, high in yield and few in impurities, the problem that a downstream process is difficult to purify and low in yield is solved, and gentamicin sulfate meeting the requirements of the China Pharmacopoeia (2020 edition) is prepared.

The invention belongs to the medical technology field, in particular discloses an oral liquid for easing mucosa edema after the radiotherapy of esophageal cancer. The oral liquid is prepared by using gentamicin sulphate, dexamethasone, 2% xylocaine, 654-2 injectam vitamin B1, vitamin B2, cimetidine and physiological saline as raw materials, respectively and proportionally compounding the raw materials according to the different characteristics of the medicaments, mixing, packaging, sealing and sterilizing. The invention has the advantages of easing oesophagus mucosal congestion and edema caused by radiotherapy, lightening swallowing pain, improving the compliance of esophageal cancer patients on radiotherapy, ensuring that the radiotherapy is smoothly finished, improving the survival quality of patients, and the like. The prescription is new, and the drug preparation is prepared from low-price raw materials with wide resources and is convenient to prepare at low drug cost. Tested by patients in esophageal cancer radiotherapy in 150 clinical cases, the total effective rate reaches 98 percent without any special toxic or side effect.

The invention discloses external-use ointment with the efficacies of promoting tissue regeneration and removing putrefaction. The external-use ointment is prepared from the following medicinal materials: radix impatientis textori, alumen, mirabilite, pollen pini, juice of Japanese banana, calcium oxide, distilled water, edible oil, fructus rosae laevigatae, distilled water, paroline, gentamicin sulphate, rhizoma bletillae, radix et rhizoma rhei, cortex phellodendri, radix sanguisorbae, myrrh, borneol, albolene, borax and phenol. Compared with the prior art, the external-use ointment is prepared by adopting the 20 traditional Chinese medicinal materials, can treat severe and lingering skin ulcer caused by various reasons, including vasculitis, phlebophlogosis, atherosclerosis obliterns, Raynaud's disease, burns and scalds, chemical burns, electrical injury, unhealed knife edge after the surgery and the like, can be widely applied to various inflammatory wound infections including viral herpes zosters, abscess and malignant ulcer, has good curative effects, and has popularization and application values.

The invention relates to the field of blood cell analysis, in particular to a diluent for blood cell analysis. The invention discloses a diluent for blood cell analysis, and is characterized in that each liter of the diluent for blood cell analysis comprises the following components: 2.0 to 5.0 g of anhydrous sodium sulfate, 5.0 to 9.0 g of sodium chloride, 0.20 to 0.35 g of disodium ethylene diamine tetraacetate, 2.0 to 3.5 g of boric acid, 0.05 to 0.10 g of gentamicin sulfate, and 0.3 to 0.8 mL of formaldehyde. The conductivity of the diluent for blood cell analysis ranges from 15 mS/cm to 20 mS/cm, the pH ranges from 6.8 to 7.8, and the osmotic pressure ranges from 300 mOsm/kg to 330 mOsm/kg. In the formula, gentamicin sulfate and formaldehyde are used for replacing a common antibacterial preservative sodium azide, so that damage to related personnel and the environment in the production and use process is avoided; and a proper ionic strength is provided, so that a stable environment is provided for counting of blood cells, and classified counting is more facilitated.