4 results about "Gentamicin sulphate" patented technology

The invention discloses a gentamicin sulfate detection reagent and a detection method, and belongs to the technical field of pharmaceutical analysis. The gentamicin sulfate detection reagent is a mixed solution containing copper sulfate, sodium citrate and sodium carbonate, the concentration of the copper sulfate in the mixed solution is 10-30 g / L, the concentration of the sodium citrate in the mixed solution is 70-200 g / L, and the concentration of the sodium carbonate in the mixed solution is 100-140 g / L. The detection method of the gentamicin sulfate comprises the following steps: uniformly mixing a detection reagent of the gentamicin sulfate with a sample to be detected in equal volume, reacting at the temperature of 20-40 DEG C for 1-4 minutes, detecting a light absorption value of 600 nm, and judging the content of the gentamicin sulfate according to a standard curve. The invention develops a novel method for rapidly detecting the total concentration of gentamicin sulfate in the visible spectrum range, which is low in detection cost and suitable for the production process of veterinary drug injection preparations.

This invention provides a human embryonic stem cell expansion culture medium, comprising DMEM / F12 medium, ITS-A, HEPES, magnesium ascorbate phosphate, non-essential amino acids, bFGF, FGF2-G3, GlutaMAX, LY-333531, gentamicin sulfate, Y27632, and CHIR99021. Y-27632 inhibits pluripotent stem cell apoptosis, improves adhesion efficiency, maintains pluripotency and colony formation ability; CHIR99021 enhances the self-renewal of pluripotent stem cells and inhibits their differentiation; LY-333531 inhibits spontaneous differentiation of pluripotent stem cells; and gentamicin sulfate reduces the risk of contamination during culture. The culture medium provided by this invention is serum-free, free of allogeneic substances, has a simple composition, and offers excellent expansion speed, effectively maintaining long-term stable passage of human embryonic stem cells, providing stable technical support for scientific research.

The invention relates to a preparation method and application of a gentamicin sulfate acellular matrix bacteriostatic slice, and the preparation method comprises the following steps: S1, taking an animal skin material, degreasing, washing and cutting to obtain a tissue material; s2, immersing the tissue material in sterile water for multiple times of ultrasonic cleaning, and then adding the tissue material into a cleaning solution for multiple times of soaking and cleaning to obtain a decellularized tissue; s3, freeze-drying the decellularized tissue, and grinding at-20 to 0 DEG C to obtain decellularized matrix powder; s4, adding the acellular matrix powder, collagen powder and gentamicin sulfate into normal saline, and uniformly mixing to obtain composite gel; and S5, pouring the composite gel into a mold, spreading the composite gel, performing pre-compression molding, and performing freeze-drying to obtain the gentamicin sulfate acellular matrix antibacterial slice. The acellular matrix treated by combining a physical means and a chemical means is cooperated with the collagen and gentamicin sulfate, so that the antibacterial sheet has the effects of high biocompatibility, long-acting and stable antibacterial effect and excellent mechanical property.

The invention relates to the field of veterinary drugs, in particular to a pharmaceutical composition for preventing and treating rabbit dermatomycosis as well as a preparation method and application thereof. The pharmaceutical composition is prepared from the following raw materials in percentage by weight: 0.5%-2% of clotrimazole, 1%-5% of 5% gentamicin sulfate powder, 20%-40% of sulfur powder, 50%-75% of talcum powder, 2%-6% of 32.5% neomycin sulfate powder and the balance of water. According to the composition, clotrimazole is introduced as a core antifungal component, a targeting action mechanism of specific inhibition of fungal cytochrome P-450 of clotrimazole is utilized, and a non-specific bactericidal effect of sulfur powder is combined, so that synergistic interaction is realized.