11 results about "Gentamicin sulphate" patented technology

The present invention belongs to the technical field of veterinary preparations, and specifically relates to a veterinary enrofloxacin compound gel ear drops and its preparation method and application. The compound gel ear drops include four active substances: enrofloxacin, triamcinolone acetonide, gentamicin sulfate, and pulegone. The preparation method is to add poloxamer, L-cysteine, and carbomer to water, stir and add benzyl alcohol to obtain solution A, dissolve low-erucic acid rapeseed oil in ethanol of more than 85%, add enrofloxacin, triamcinolone acetonide, pulegone, and gentamicin sulfate to obtain solution B; slowly add solution B to the gel matrix of solution A to obtain solution C, and then filter to obtain the compound gel. Pharmacodynamic experiments have confirmed that it has a good therapeutic effect on inflammation of the external ear canal of pets; accelerated experiments have confirmed that the compound gel is stable in nature, can be stored for a long time, and is suitable for further expansion of production.

The invention discloses a method for removing companion bacteria in tissue culture subculture multiplication culture of clerodendrum trichotomum and application of the companion bacteria in tissue culture subculture multiplication culture of clerodendrum trichotomum. The method comprises the following steps: carrying out pretreatment and disinfection treatment on explants of stem segments with buds of clerodendrum trichotomum, and establishing a sterile system; inoculating the explants to an induction medium, culturing for 14 days to form calluses, transferring the calluses to a redifferentiation medium, and culturing for 7 days to form adventitious buds; the adventitious buds are transferred to a proliferation culture medium, companion fungus pollution occurs during proliferation culture, a sterile gentamicin sulfate solution with the concentration of 800 mg / L is adopted for treatment, the companion fungus clearance rate reaches 100%, the clerodendrum trichotomum sterile seedlings can normally grow, plants are robust and free of yellowing and vitrification phenomena, and the proliferation coefficient reaches up to 16 (24). According to the method, the problem that in a traditional mode, the reproduction period is greatly prolonged due to pollution, namely waste reproduction, is effectively solved, waste of valuable and rare plant materials is reduced, and the tissue culture seedling culture success rate is greatly increased.

The invention discloses a gentamicin sulfate detection reagent and a detection method, and belongs to the technical field of pharmaceutical analysis. The gentamicin sulfate detection reagent is a mixed solution containing copper sulfate, sodium citrate and sodium carbonate, the concentration of the copper sulfate in the mixed solution is 10-30 g / L, the concentration of the sodium citrate in the mixed solution is 70-200 g / L, and the concentration of the sodium carbonate in the mixed solution is 100-140 g / L. The detection method of the gentamicin sulfate comprises the following steps: uniformly mixing a detection reagent of the gentamicin sulfate with a sample to be detected in equal volume, reacting at the temperature of 20-40 DEG C for 1-4 minutes, detecting a light absorption value of 600 nm, and judging the content of the gentamicin sulfate according to a standard curve. The invention develops a novel method for rapidly detecting the total concentration of gentamicin sulfate in the visible spectrum range, which is low in detection cost and suitable for the production process of veterinary drug injection preparations.

This invention provides a human embryonic stem cell expansion culture medium, comprising DMEM / F12 medium, ITS-A, HEPES, magnesium ascorbate phosphate, non-essential amino acids, bFGF, FGF2-G3, GlutaMAX, LY-333531, gentamicin sulfate, Y27632, and CHIR99021. Y-27632 inhibits pluripotent stem cell apoptosis, improves adhesion efficiency, maintains pluripotency and colony formation ability; CHIR99021 enhances the self-renewal of pluripotent stem cells and inhibits their differentiation; LY-333531 inhibits spontaneous differentiation of pluripotent stem cells; and gentamicin sulfate reduces the risk of contamination during culture. The culture medium provided by this invention is serum-free, free of allogeneic substances, has a simple composition, and offers excellent expansion speed, effectively maintaining long-term stable passage of human embryonic stem cells, providing stable technical support for scientific research.

The invention discloses hydrogel with pH responsiveness as well as a preparation method and application of the hydrogel. The preparation method comprises the following steps: stirring and dispersing the hyaluronic acid, the N, N-diisopropylethylamine, the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and the N-hydroxysuccinimide into water; gentamicin sulfate is added, and stirring is continued; performing freeze-drying; carrying out hydration to form hydrogel; soaking in a mesoporous silica suspension; and soaking in a bromothymol blue solution. According to the invention, hyaluronic acid, gentamicin sulfate, mesoporous silica and bromothymol blue are combined to prepare the hydrogel with pH responsiveness. The hydrogel can release gentamicin sulfate through hydrolysis to effectively resist bacteria, and the color of the loaded pH sensitive indicator bromothymol blue can also generate characteristic change along with the change of the pH value of the wound microenvironment, so that the visual monitoring of the wound state is realized.

1. The name of the design product: medicine packaging box (gentamicin sulfate eye drops). 2. The use of the design product: used for the packaging of medicine gentamicin sulfate eye drops. 3. The design points of the design product: the combination of patterns and colors. 4. The picture or photo that best indicates the design points: perspective view. 5. The design protected contains colors.

The invention relates to a preparation method and application of a gentamicin sulfate acellular matrix bacteriostatic slice, and the preparation method comprises the following steps: S1, taking an animal skin material, degreasing, washing and cutting to obtain a tissue material; s2, immersing the tissue material in sterile water for multiple times of ultrasonic cleaning, and then adding the tissue material into a cleaning solution for multiple times of soaking and cleaning to obtain a decellularized tissue; s3, freeze-drying the decellularized tissue, and grinding at-20 to 0 DEG C to obtain decellularized matrix powder; s4, adding the acellular matrix powder, collagen powder and gentamicin sulfate into normal saline, and uniformly mixing to obtain composite gel; and S5, pouring the composite gel into a mold, spreading the composite gel, performing pre-compression molding, and performing freeze-drying to obtain the gentamicin sulfate acellular matrix antibacterial slice. The acellular matrix treated by combining a physical means and a chemical means is cooperated with the collagen and gentamicin sulfate, so that the antibacterial sheet has the effects of high biocompatibility, long-acting and stable antibacterial effect and excellent mechanical property.

The invention relates to the field of biological medicine, in particular to an antithrombin III determination kit and a preparation method thereof. The antithrombin III determination kit comprises a reagent 1, a reagent 2, an ATIII calibration product and an ATIII quality control product. The reagent 1 is prepared from a Tris-HCl buffer solution, calcium chloride, bovine serum albumin, potassium ferrocyanide, gentamicin sulfate, ProClin 300, recombinant human thrombin and a nano blocking agent; the reagent 2 is prepared from a Tris-HCl buffer solution, S-2238, gentamicin sulfate and ProClin 300; the ATIII calibrator is prepared from a synthetic plasma matrix, recombinant human ATIII, natural ATIII, ProClin 300, a blood coagulation inhibitor, protamine and trehalose; the ATIII quality control product is prepared from a synthetic plasma matrix, recombinant human ATIII, natural ATIII, ProClin 300, a blood coagulation inhibitor, trehalose and mannitol, according to the scheme, the method is suitable for a full-automatic blood coagulation analysis platform and conforms to the development trend of in-vitro diagnostic reagents.

The application discloses a decahydrofluorene type alkaloid compound, a preparation method thereof and application thereof in preparation of antibacterial drugs. The structural formula of the alkaloid compound is shown as formula (I). The compound is isolated from mycelium of marine fungi Microascus sp. SCSIO 41821. Experiments prove that 10-13 of the application have the activity of inhibiting protein tyrosine phosphatase PTPSig; compounds 4, 9 and 10 have significant inhibitory activity on 7 strains of bacteria, and the MIC value ranges from 0.1 to 0.8 mu g / mL, which is slightly stronger than that of positive drugs gentamicin sulfate and vancomycin, and the compounds can be applied to protein tyrosine phosphatase inhibitors or antibacterial drugs.

The invention relates to the field of veterinary drugs, in particular to a pharmaceutical composition for preventing and treating rabbit dermatomycosis as well as a preparation method and application thereof. The pharmaceutical composition is prepared from the following raw materials in percentage by weight: 0.5%-2% of clotrimazole, 1%-5% of 5% gentamicin sulfate powder, 20%-40% of sulfur powder, 50%-75% of talcum powder, 2%-6% of 32.5% neomycin sulfate powder and the balance of water. According to the composition, clotrimazole is introduced as a core antifungal component, a targeting action mechanism of specific inhibition of fungal cytochrome P-450 of clotrimazole is utilized, and a non-specific bactericidal effect of sulfur powder is combined, so that synergistic interaction is realized.

The invention provides a preparation method of gentamicin procaine vitamin B12 granules, which comprises the following steps: A) mixing aspartame, sucrose powder and procaine hydrochloride, sieving, pouring the sieved material into an efficient mixing granulator for mixing, pouring the mixed solution, mixing, and discharging to obtain a soft material; the mixed solution comprises a vitamin B12 aqueous solution, a lemon yellow aqueous solution and a gentamicin sulfate solution; b) adding the soft material into a hopper of a swinging granulator, starting a pelleting pot, putting the swung wet granules into the pelleting pot, spraying purified water, and sieving to obtain wet granules; and C) drying the wet granules, finishing the granules, and totally mixing to obtain the traditional Chinese medicine composition. According to the preparation method of the gentamicin procaine vitamin B12 particles, the advantages that the prepared gentamicin procaine vitamin B12 particle product is less in fine powder, good in fluidity and stable in quality can be realized.