Lactic acid bacteria complexing agent for removing earthy smell of eriocheir sinensis and preparing method of lactic acid bacteria complexing agent

A technology of Chinese mitten crab and lactic acid bacteria is applied in the field of biochemistry to achieve the effects of environmental friendliness, simple production process and broad application prospects.

Active Publication Date: 2019-08-23
苏州宏螯生物农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no systematic research reports in this field at home and abroad.

Method used

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  • Lactic acid bacteria complexing agent for removing earthy smell of eriocheir sinensis and preparing method of lactic acid bacteria complexing agent
  • Lactic acid bacteria complexing agent for removing earthy smell of eriocheir sinensis and preparing method of lactic acid bacteria complexing agent

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Embodiment 1～4

[0057] Adopt the following method to prepare the lactic acid bacteria compound provided by the invention according to the mass percent of each component in table 1 and the concentration of live bacteria respectively:

[0058] Table 1

[0059]

[0060] The preparation steps of the lactic acid bacteria compound preparation used in each embodiment are as follows:

[0061] (1) Preparation of Bifidobacterium bifidum bacteria powder: under sterile conditions, inoculate the Bifidobacterium bifidum strain into liquid medium, place it in a constant temperature shaker at 37°C for activation for 18 hours, and then inoculate the activated Bifidobacterium bifidum Put the mycobacterium strain into a fermenter, carry out secondary fermentation at 28° C. according to 5% inoculation amount for 36 hours, adjust the pH to 4 with citric acid, and freeze-dry to obtain Bifidobacterium bifidum powder. The formula of the fermentation medium is (mass fraction): 37% of soluble starch, 30% of soybea...

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Abstract

The invention discloses a lactic acid bacteria complexing agent for removing the earthy smell of eriocheir sinensis. The complexing agent comprises bifidobacterium bifidum, embryo lactic acid bacteria, bifidobacterium infantis and a buffering agent. The total live bacteria concentration of the bifidobacterium bifidum, embryo lactic acid bacteria and bifidobacterium infantis is larger than or equalto 1.1 million / g. In the aquaculture process, by feeding the lactic acid bacteria complexing agent, the content of flavor substances such as limonene in the eriocheir sinensis can be effectively increased, the content of substances such as trimethylamine, geosmin (GSM) and 2-methylisoborneol (2-MIB) with the earthy smell in the eriocheir sinensis is effectively reduced, and the quality of the eriocheir sinensis is remarkably improved.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to a lactic acid bacteria compound agent, in particular to a lactic acid bacteria preparation for removing the earthy smell of mitten crab sinensis and an application thereof. Background technique [0002] Chinese mitten crab (Eriocheirsinensis), also known as Chinese mitten crab, is an important breeding economic crab. At present, the breeding mode of Chinese mitten crab is constantly improving and developing, and the breeding output is also increasing year by year. In 2014, the production of Chinese mitten crab hepatopancreas was as high as 796,500 tons, with a total output value of about 50 billion yuan. Chinese mitten crab farming has become an effective means to increase employment levels and improve people's quality of life. [0003] The quality of Chinese mitten crab is the key factor to determine its economic value and industrial development. Due to the influence of factors such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18C12N1/20C12R1/01
CPCA23K50/80A23K10/18C12N1/20A23V2400/529A23V2400/517
Inventor 胡鲲陈献练小军
Owner 苏州宏螯生物农业发展有限公司
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