In-vivo nondestructive sampling method for eriocheir sinensis and application thereof

Abstract

The present invention discloses an in-vivo nondestructive sampling method for eriocheir sinensis and an application thereof. Fertilized eggs of egg-carrying parents of eriocheir sinensis are sampled,and DNAs and RNAs are extracted from the fertilized eggs for detection of a relevant pathogen. The parents free of the detection object pathogen are segregated for breeding, and then specific pathogenfree (SPF) megalopae of eriocheir sinensis are obtained through captive breeding. The method has the advantages that the bodies of parents are not injured by sampling, thereby guaranteeing normal eggcarrying and hatching of the parent crabs, and the obtained megalopae of eriocheir sinensis are specific pathogen free. The in-vivo nondestructive sampling method for eriocheir sinensis solves the difficult problem of in-vivo nondestructive sampling in SPF breeding of eriocheir sinensis and provides a simple and feasible approach for SPF breeding of eriocheir sinensis. Thus, healthy SPF seeds areexpected to be bred, so that the economic benefit of breeding of eriocheir sinensis can be increased.

Description

technical field [0001] The invention relates to the field of SPF seedling cultivation of economic crustaceans and other application fields requiring detection of specific pathogens, in particular to a non-destructive sampling method for shrimps and crabs with ovipositing habits and the application of SPF seedling cultivation. Background technique [0002] Chinese mitten crab (Eriocheir sinensis), also known as river crab, is an important economic aquatic animal in my country. It is widely distributed in rivers and lakes in the north and south of China. The known viruses of Chinese mitten crab include: reovirus (EsRV816, 905 and 2012), bunya virus (EsBV), and white spot syndrome virus (WSSV). These viruses all have the characteristics of vertical transmission. The known Eriocheir sinensis virus can make Eriocheir sinensis pathogenic and even fatal under certain conditions. [0003] Due to the prevalence of White Spot Syndrome Virus (WSSV), Specific pathogen free (SPF, Speci...

AI Technical Summary

Problems solved by technology

SPF seedlings require that the parents do not carry specific pathogens, and the parents must first be tested for specific pathogens. When live sampling is performed on the economical crustacean breeding parents for molecular testing, because the crustaceans are covered with a hard shell, body surface sampling cannot be performed. Therefore, the method of taking muscle by amputating limbs is usually used. This method causes the disability of the sampling target individual, the decline in vitality, and even some individuals die of infection. Therefore, this method is not suitable for the pathogens of SPF seedlings of some shrimp and crab species that require long-term egg incubation. detection

And if you want to test other tissues, such as hepatopancreas, sperm ovary, etc., you need to kill the test parent, which is even more unfeasible.