Application of a non-destructive sampling method for Chinese mitten crab

Abstract

The invention discloses a non-destructive sampling method and application of living body of Chinese mitten crab. By sampling the fertilized eggs of the ovipositous parents of the Chinese mitten crab, the DNA and RNA thereof are extracted to detect related pathogens, and the parents not containing the detection target pathogen are tested. SPF megalopa of Eriocheir sinensis was obtained by isolation cultivation and artificial propagation. The method has the following advantages: the sampling does not damage the mother body, ensures the normal egg hatching of the parent crab, and the obtained megalopa of the Chinese mitten crab does not carry specific pathogens. The present invention solves the problem of non-destructive live sampling in SPF seedling cultivation of Chinese mitten crab, provides a simple and feasible way for SPF seedling cultivation of Chinese mitten crab, and is expected to cultivate SPF healthy seedlings and improve the economic benefits of Chinese mitten crab cultivation .

Description

technical field [0001] The invention relates to the field of SPF seedling cultivation of economic crustaceans and other application fields requiring detection of specific pathogens, in particular to a non-destructive sampling method for shrimps and crabs with ovipositing habits and the application of SPF seedling cultivation. Background technique [0002] Chinese mitten crab (Eriocheir sinensis), also known as river crab, is an important economic aquatic animal in my country. It is widely distributed in rivers and lakes in the north and south of China. The known viruses of Chinese mitten crab include: reovirus (EsRV816, 905 and 2012), bunya virus (EsBV), and white spot syndrome virus (WSSV). These viruses all have the characteristics of vertical transmission. The known Eriocheir sinensis virus can make Eriocheir sinensis pathogenic and even fatal under certain conditions. [0003] Due to the prevalence of White Spot Syndrome Virus (WSSV), Specific pathogen free (SPF, Speci...

AI Technical Summary

Problems solved by technology

SPF seedlings require that the parents do not carry specific pathogens, and the parents must first be tested for specific pathogens. When live sampling is performed on the economical crustacean breeding parents for molecular testing, because the crustaceans are covered with a hard shell, body surface sampling cannot be performed. Therefore, the method of taking muscle by amputating limbs is usually used. This method causes the disability of the sampling target individual, the decline in vitality, and even some individuals die of infection. Therefore, this method is not suitable for the pathogens of SPF seedlings of some shrimp and crab species that require long-term egg incubation. detection

And if you want to test other tissues, such as hepatopancreas, sperm ovary, etc., you need to kill the test parent, which is even more unfeasible.