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Apple rootstock tissue culture rapid seedling raising method

A tissue culture and rootstock technology, applied in the field of plant tissue culture, can solve problems such as not being able to meet actual production needs, and achieve the effect of reducing production difficulty and shortening time

Inactive Publication Date: 2017-11-24
济南浩隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Can not meet the actual production needs

Method used

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  • Apple rootstock tissue culture rapid seedling raising method
  • Apple rootstock tissue culture rapid seedling raising method
  • Apple rootstock tissue culture rapid seedling raising method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1. Qingzhen No. 1 tissue culture rapid seedling raising method

[0044] First, the in vitro budded branches are sterilized to obtain sterile seedlings. The method is as follows: 1. Pick and cut annual robust branches with young buds during the dormant period, soak them in bromogeramine for 20 minutes, scrub the surface of the branches with a brush (especially near the buds), and then rinse them with running water. 2. Use fruit branch scissors to cut obliquely into 6-8cm stem segments in water, make sure each segment has 2-3 buds, and place them in a clean tissue culture bottle. 3. Fill a 240ml tissue culture bottle with 3 / 4 pure water, cover it tightly with a lid without a gas-permeable membrane, sterilize it in an autoclave at 125°C for 30 minutes to prepare sterile water, and cool it for later use. 4. Open the inoculator sterilizer placed in the ultra-clean workbench, insert tools such as surgical scissors, scalpels, and gun-like tweezers into the inoculat...

Embodiment 2

[0054] Embodiment two, the inventive method compares with conventional method

[0055] 1. Test materials and methods

[0056] 1.1 Materials

[0057] Green Anvil No. 1

[0058] 1.2 Test method

[0059] The conventional culture and the culture method of the present invention are adopted respectively; the method of the present invention is the method of Example 1. The general method is as follows:

[0060] Select the leaves of the explants, wash them with detergent, and rinse them under running water for about 30 minutes; then wash them with 75% alcohol and 1g·L -1 The mercuric chloride solution was disinfected for 30s and 8min, and then rinsed with sterile water for 3-5 times. Place the leaves on sterile filter paper, cut off the leaf margins and tips with a scalpel, and cut them into leaf blocks.

[0061] The culture medium and culture time used in each stage are as follows:

[0062] The basic medium is MS.

[0063] Differentiation medium: MS+5.0mg·L -1 6-BA+0.3mg·L -...

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Abstract

The invention provides an apple rootstock tissue culture rapid seedling raising method. Stems serve as explants, an adventitious bud induction differentiation culture medium, a subculture multiplication culture medium, a rooting culture medium and the like are optimized, the multiplication coefficient of adventitious buds is 4.2, the final rooting rate reaches 100%, and the average rooting number reaches 7.3. By controlling specific parameters of each stage of tissue culture seedling greenhouse domestication, the survival rate of transplanting reaches 90% 30 days after seedling hardening.

Description

technical field [0001] The invention relates to a rapid seedling raising method of apple rootstock tissue culture, which belongs to the technical field of plant tissue culture. Background technique [0002] "Qingzhen No. 1" is a new apple rootstock strain bred by Qingdao Academy of Agricultural Sciences and Shandong Agricultural University with Pingyi sweet tea as the female parent and columnar apple line CO as the male parent. In addition to the traits, it also has excellent dwarfing performance. "Qingzhen No. 1" has a well-developed root system, strong tree body fixation after grafting varieties, obvious dwarfing effect, easy flowering, early high yield and strong stress resistance, and the promotion effect in Shaanxi, Shandong and Xinjiang is very good, and it is very developed Potential rootstock varieties independently developed by my country. [0003] At present, "Qingzhen No. 1" mainly relies on sowing and breeding. Conventional sowing and breeding need to occupy a l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G9/10A01H4/00C05G3/00A01G31/00
CPCA01G31/00A01H4/008C05D9/00C05F11/00C05G3/00
Inventor 李成刚张娟
Owner 济南浩隆生物科技有限公司
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