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Domestication method of strawberry tissue culture plants

A technique for tissue culture seedlings and strawberries is applied in the field of domestication of strawberry tissue culture seedlings to achieve the effects of high domestication survival rate, improved survival rate and easy to master.

Inactive Publication Date: 2019-01-15
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Purpose of the invention: Aiming at the problem of the survival rate of strawberry field mother seedlings in the prior art, the present invention provides a simple and efficient method for domesticating strawberry tissue culture seedlings in summer, which is helpful for establishing subtropical regions

Method used

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  • Domestication method of strawberry tissue culture plants
  • Domestication method of strawberry tissue culture plants
  • Domestication method of strawberry tissue culture plants

Examples

Experimental program
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Effect test

Embodiment

[0018] Embodiment A kind of method that improves the domestication of strawberry tissue culture seedling (taking Hongyan as an example)

[0019] 1. Uncapped domestication: Select strawberry tissue culture seedlings with 4-5 leaves and a plant height of no more than 3 cm, and open the cover for domestication in the laboratory for 3 days, then transfer the uncovered tissue cultured seedlings to a greenhouse for another 1 day.

[0020] 2, prepare substrate: one day before transplanting, substrate (vermiculite: perlite: peat) is packed into 50 hole trays and watered thoroughly, each embodiment is done 2 kinds of treatments, a kind of 85% carbendazim with 800 times The wettable powder solution was disinfected, and the other was not. 20 trays were processed for each example.

[0021] Table 1 embodiment matrix proportioning

[0022]

[0023]

[0024] 3. Root washing: Take out the tissue culture seedlings in the bottle and wash the medium of the roots with running water. When ...

Embodiment 2

[0030] From the above examples, it was found that the strawberry tissue-cultured seedlings in the sterilized matrix of Example 1 (vermiculite: perlite: peat=1:1:3) had the highest survival rate, up to 98%. Example 2 (vermiculite: perlite: peat=1:1:1) the survival rate of strawberry tissue culture seedlings in the sterilized and non-sterilized substrates is very low, mainly because the ratio of vermiculite, perlite and peat is not high. Suitable for the growth of strawberry tissue culture seedlings, the substrate in Example 2 is loose, and the water retention performance is not good. The number of new leaves is small, the petiole elongation is slow, the dry matter content is low, and the plant stretches 6.6cm, which is relatively short. Next in Example 3 (vermiculite:perlite:peat=1:1:2), the survival rate of strawberry tissue culture seedlings in the sterilized substrate was 96.4%, and the survival rate of non-sterilized strawberry tissue culture seedlings was 79.6%. Example 4...

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Abstract

The invention discloses a domestication method of strawberry tissue culture plants. The method includes steps including cover opening domestication of the tissue culture plants, re-domestication in agreenhouse, root cleaning, cultivation and management. The tissue culture plants are domesticated twice before transplantation to improve the adaptiblity, a more suitable cultivation condition is provided for domestication, and via the method, domesticated strawberry is high in survival rate, short in rejuvenation period, fast in growth and improved in quality; and the method is easy to master, adomestication is easy to construct, the cost is lower, repeated use is allowed, and the greenhouse can be used to plant other crops after domestication.

Description

technical field [0001] The invention belongs to a method for raising seedlings of horticultural crop seedlings, in particular to a method for domesticating strawberry tissue culture seedlings. Background technique [0002] Strawberry is a perennial herb of the Rosaceae Strawberry genus. It belongs to one of the seven major fruits in the world today and is known as the "Queen of Fruits". It is cultivated all over the country. At present, Hongyan strawberry is the main variety in Jiangsu. The plant of Hongyan strawberry has strong growth, large fruit, high sugar content, high hardness and storage resistance. Therefore, it is deeply loved by consumers, but the natural reproduction rate is very low. Due to the rapid development of production, strawberry seedlings are in great demand. Traditionally, strawberry seedlings are produced using stolons and divisions, but long-term use of this vegetative method will cause strawberries to be infected with viruses, accumulate and degrad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G24/15A01G24/28
CPCA01G31/00A01G24/15A01G24/28
Inventor 颜志明冯英娜孙颖王媛花解振强王全智杨宝林董慧
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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