Tissue culture and rapid propagation method of medicinal plant stemona japonica and application of tissue culture and rapid propagation method

Abstract

The invention relates to the field of medicinal plant tissue culture, in particular to a tissue culture and rapid propagation method of a medicinal plant stemona japonica, which comprises the following steps: 1) explant disinfection: selecting a stem section with buds of the stemona japonica, cleaning, and disinfecting by using 75% alcohol, a saturated bleaching powder solution, a 8% hydrogen peroxide solution and 0.025% mercury bichloride in sequence; 2) cluster bud induction: inoculating the disinfected explants into an induction culture medium for culture; 3) proliferation of cluster buds: inoculating the induced cluster buds into a proliferation culture medium according to polarity for culture; and 4) rooting of the cluster buds: cutting the proliferated cluster buds into single buds, and inoculating the single buds to a rooting culture medium according to polarity for culture. According to the method for tissue culture and rapid propagation of the radix stemonae, the disinfection contamination rate, the death rate, the proliferation withering and yellowing rate and the rooting withering and yellowing rate of the radix stemonae are far lower than those of an existing disclosed technical scheme, the 50-day induction rate, the 35-day proliferation coefficient, the 30-day rooting rate and the 50-day domestication survival rate are all higher than those of the prior art, and rapid propagation of the radix stemonae can be achieved.

Description

technical field [0001] The invention relates to the field of tissue culture of medicinal plants, in particular to a method for tissue culture and rapid propagation of medicinal plants. Background technique [0002] Stemona tuberosa Lour., also known as Stemona tuberosa Lour., also known as Stemona tuberosa Lour., is a perennial herb belonging to Stemona tuberosa Lour. It mainly grows on hillsides at an altitude of 370-2240 meters. Under the jungle, by the stream, along the road, in valleys and damp rocks, it is distributed in all provinces and regions south of the Yangtze River Basin in my country, and also in the Indochina Peninsula, the Philippines and northern India. The roots are used as medicine, which is one of the base plants of Chinese medicinal materials. It has the effects of moistening lungs, lowering qi, relieving coughs, and killing insects and lice. It is mainly used for chronic cough, tuberculosis cough, sudden cough; externally used for head lice, body lice, ...

AI Technical Summary

Problems solved by technology

[0004] At present, the main source of the medicinal materials of Baibu is wild resources. In recent years, wild resources have been introduced and planted in Yunnan, Guangxi, Guizhou and other places. The propagation of Baibu mainly depends on seeds, but the hybridization between varieties in seed propagation leads to impure varieties. The seed setting rate is low, the seed germination rate is low, etc. At the same time, there are many varieties of Baibu, and the content of active ingredients such as alkaloids among the varieties varies greatly, which makes the quality of the currently artificially propagated Baibu medicinal materials and seedlings uneven.

In addition, Libu can be propagated by cuttings and vines, but the rooting rate is low, and the reproduction rate is low, and the reproduction cycle is long.

[0005] In recent years, some units have also carried out tissue culture propagation of hundreds of plants, but the existing tissue culture technology has a high rate of disinfection pollution, and when high concentrations of mercuric chloride or low concentrations are used for long-term disinfection, it is easy to cause a large number of explants to die. Simultaneously also there is multiplication coefficient and rooting rate low in cultivation process simultaneously, in multiplication and rooting cultivation process, the situation of a large amount of seedlings withered yellow or even withered white makes seedling loss rate higher, and the rooted seedlings of prior art, root system is rough and easy break off

Images