Method for culture and plant regeneration of ormosia microphylla in-vitro embryo

A technology for culturing small-leafed red beans and embryos, which is applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of hard seeds, low survival rate, poor water permeability of seed coats, etc. The effect of high, short germination time

Inactive Publication Date: 2014-04-02
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

In the prior art, the small-leaf red bean has few natural resources, low survival rate, flat oval seed coat is hard, waxy, and the germination is uneven. Some seeds germinate after 2 years in the soil, and the seeds are easily eaten by rats and birds; the seeds are hard , the water permeability of the seed coat is poor, the seed coat is not easy to absorb water and germinate, and it must be germinated after special germination treatment, natural renewal is difficult, natural reproduction ability, and diffusion ability are not strong. Cultivate high-quality seedlings and efficiently conserve rare and endangered local species of small-leaf red bean resources, adjust the structure of forest species and tree species, and promote sustainable forestry development. Provide technical support for the effective protection and management of tree species, which has important theoretical significance and high economic value development and utilization prospects

Method used

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  • Method for culture and plant regeneration of ormosia microphylla in-vitro embryo
  • Method for culture and plant regeneration of ormosia microphylla in-vitro embryo
  • Method for culture and plant regeneration of ormosia microphylla in-vitro embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Method of obtaining materials: select the pods with full grains that were strong in the year as the material, and wrap the picked pods with a damp cloth, and bring them back to the laboratory to store in a refrigerator at 4°C for later use;

[0037] 2) Preparation of medium

[0038] Prepare embryo induction medium, primary bud induction medium, bud proliferation medium, strong seedling medium, rooting medium, and add Su: 20 g·L to the medium -1， Ag: 6.0g·L -1 , PH value: 5.8, the addendum is activated carbon Ac; the thickness of the medium is 1.4 cm; the use of a good air-permeable bottle material can reduce the water supply of the culture, thereby preventing the vitrification of the woody plant growth process and ensuring Improve the quality of cultivated seedlings.

[0039] Among them, embryo induction medium: 1 / 2MS (major elements halved) + 1.0mg·L -1 BA + 0.5mg·L -1 IAA + 0.2mg·L -1 NAA +6.0g·L -1 Ag +20g·L -1 Su +1.0 g·L -1 Ac;

[0040] Primary bud induction medium: W...

Embodiment 2

[0052] 1) Method of obtaining materials: select the pods with full grains that were strong in the year as the material, and wrap the picked pods with a damp cloth, and bring them back to the laboratory to store in a refrigerator at 4°C for later use;

[0053] 2) Configuration of medium

[0054] Prepare embryo induction medium, primary bud induction medium, bud proliferation medium, strong seedling medium, and rooting medium. Su and Ag are added to the medium separately, pH value: 5.7, and the addition is activated carbon Ac ; The thickness of the culture medium is 1.6 cm; (and the use of a good air-permeable bottle material can reduce the water supply of the culture, thereby preventing the vitrification of the woody plant growth process and ensuring the quality of the cultivated seedlings).

[0055] Among them, embryo induction medium: 1 / 2MS (major elements halved) + 1.5mg·L -1 BA + 1.0mg·L -1 IAA + 0.3mg·L -1 NAA + 6.0g·L -1 Ag +20g·L -1 Su +1.5 g·L -1 Ac.

[0056] Primary bud indu...

Embodiment 3

[0068] 1) Material collection method: select the pods with full grains that were firm in the year as materials, wrap the picked pods with a damp cloth, and bring them back to the laboratory to store in a refrigerator at 4°C for later use;

[0069] 2) Preparation of medium

[0070] Prepare embryo induction medium, primary bud induction medium, bud proliferation medium, strong seedling medium, and rooting medium respectively. Su and Ag are added to the medium separately, pH value: 5.9, and the addition is activated carbon Ac ; The thickness of the medium is 1.5 cm;

[0071] Among them, embryo induction medium: 1 / 2MS+ 0.5mg·L -1 BA + 0.4mg·L -1 IAA + 0.1mg·L -1 NAA + 6.0g·L -1 Ag +20g·L -1 Su +1.2 g·L -1 Ac;

[0072] Primary bud induction medium: WPM+1.0mg·L -1 BA + 0.4mg·L -1 KT + 0.4mg·L -1 IAA+0.4mg·L -1 NAA+ 6.0g·L -1 Ag +20g·L -1 Su +1.2 g·L -1 Ac;

[0073] Bud propagation medium: WPM+1.0mg·L -1 BA + 0.5mg·L -1 KT + 0.1mg·L -1 TDZ + 0.4mg·L -1 NAA + 6.0g·L -1 Ag +20g·L -1 Su +1.2...

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Abstract

The invention belongs to the method for cultivating economic forests and particularly relates to a method for culture and plant regeneration of an ormosia microphylla in-vitro embryo. According to the method disclosed by the invention, the culture and plant regeneration of the ormosia microphylla in-vitro embryo is completed by adopting embryo induced culture, primary bud inducted culture, bud multiplication culture, strong seedling culture, and root induction culture. The method for culture and plant regeneration of the ormosia microphylla in-vitro embryo is higher in expanding propagation coefficient, short in germination time and high in propagation speed, and has an important theoretical significance and higher economic value as well as development utilization prospect for efficiently nursing rare and endangered ormosia microphylla resources, through the strong seedling culture, a grown-up seedling is high in survival rate after being transplanted, and the seedling is healthy and strong as well as tall and straight with good growth vigor.

Description

technical field [0001] The invention belongs to a cultivation method of an economic forest, in particular to a method for in vitro embryo culture and plant regeneration of red bean with small leaves. Background technique [0002] Small leaf red beans ( Ormosia micropylla ) for Papilionaceae Papilionaceae Ormosia Ormosia G. Jacks. Tree species, rare species. Small-leaved red bean is a declining type, and it is difficult to regenerate naturally under the forest. The ability of natural reproduction and spread is not strong. With the development and succession of the community, it will be replaced by other species. The small-leaf red bean wood is hard and of good quality. The difference between the sapwood and the heartwood is obvious. The sapwood is light yellowish brown, and the heartwood has a large proportion, which is deep purple to purple black. It is called "rosewood" by the people. , dry shrinkage, strong corrosion resistance, beautiful color and pattern, is a spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何碧珠林蔚朱萍凌昌
Owner FUJIAN AGRI & FORESTRY UNIV
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