Complete nutrition formula germination accelerating method for tobacco seed
A technology of tobacco seeds and nutritional formula, applied in germination equipment, etc., can solve the problems of irregular seedling emergence, slow seedling emergence, and low seedling emergence rate, and achieve the effects of neat seedling emergence, rapid growth and development of seedlings, and high seedling emergence rate
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[0025] (1) Experimental method
[0026] (1) Tobacco seeds to be tested: The variety to be tested is China Tobacco 201 first-class fine variety, provided by Qingdao China Tobacco Seed Co., Ltd.
[0027] (2) Culture medium setting for germination treatment: gibberellin (GA 3 ) set four concentration gradients of 0, 100mg / L, 300mg / L, and 500mg / L; naphthaleneacetic acid (NAA) set six concentrations of 0, 2mg / L, 5mg / L, 10mg / L, 20mg / L, and 50mg / L Gradient; basic medium is H 2 O, MS liquid medium (Murashige and Skoog, 1962), three factors were completely random combination, 48 treatments, three repetitions.
[0028] (3) Accelerating germination: The seeds are first sterilized with 0.5% copper sulfate solution by weight for 15 minutes, then rinsed several times with distilled water, dried at 30°C to a water content of about 10%, and then accelerated germination with the above prepared culture solution. The germination is carried out in the germination tank, and the air compressor i...
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