Method for rapidly propagating Camelia sinensis cv. Chongqing pipacha

Abstract

The invention discloses a method for rapidly propagating Camelia sinensis cv. Chongqing pipacha. The method comprises the following steps of: sequentially cleaning an explant of a stem segment with buds of an indoor cutting seedling of the Camelia sinensis cv. Chongqing pipacha by using soapy water, soaking in carbendazim, and washing by using running water, completely absorbing the moisture on the surface of the explant, and removing small leaves from outer layers of the buds and the stem segment; sequentially disinfecting the explant by using alcohol and mercury bichloride under aseptic conditions, washing by using sterile water, inoculating into a Murashige and Skoog (MS) culture medium, culturing, and screening out a survival aseptic material; transferring the aseptic material into a startup culture medium, culturing and inducing into buds; and transferring the germinant aseptic material into an enrichment culture medium, and continuing to culture to obtain cluster buds with the average multiplication coefficient of 3.31 to 4.86. By the method, the aseptic material of which the sterilization survival rate is up to 88.5 percent can be obtained, the germination time is short, the multiplication rate is high, the cluster buds of which the average multiplication coefficient is up to 4.86 can be obtained within three months, and excellent characters of a genotype and a phenotype of the original clone can be maintained.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of plants, and in particular relates to a method for rapid propagation of loquat tea. Background technique [0002] Loquat tea (Camelia sinensis cv. Chongqing pipacha) belongs to the evergreen trees of the genus Camelia (Theaceae) in the family Theaceae, and is mainly distributed in Chongzhou City, Dayi County and other areas in Sichuan Province. Loquat tea has a high yield and good quality. It is one of the provincial traditional fine varieties recognized by the Sichuan Provincial Tea Tree Breeding Committee, and has been rated as a geographical indication agricultural product of Sichuan Province. In addition, loquat tea has a large corolla, golden stamens, strong aroma, and a long flowering period, which is open almost throughout the winter, and has good ornamental value. [0003] At present, the propagation methods of loquat tea mainly rely on traditional seed propagation and cutting...

AI Technical Summary

Problems solved by technology

All there is following defective in these two kinds of methods: (1) the cycle of propagation is long, and cutting propagation generally needs 12-14 months, and the cycle of seed propagation is longer; (2) reproduction coefficient is relatively low, especially cutting propagation; (3) It is not easy to maintain good characters by natural breeding of seeds; (4) easily subject to seasonal and geographical restrictions, because planting loquat tea requires sufficient moisture and suitable pH

[0004] Although there are a small amount of reports on the rapid propagation method of plants in the prior art, 1) there is no information about the in vitro rapid propagation of loquat tea at present; 2) in view of the genetic characteristics of each plant variety, the disclosed rapid propagation method cannot be used directly. In the rapid propagation of loquat tea