The invention belongs to the field of microbial 
fermentation, and particularly relates to a microbial 
fermentation feed and a preparation method and application thereof. The preparation method of the microbial 
fermentation feed includes the following steps that a 
plant base material is smashed, then a complex 
enzyme preparation is added and mixed for 
hydrolysis, and an enzymolysis product is obtained; the enzymolysis product is mixed with 
rumen content for anaerobic liquid fermentation, and a fermentation product 1 is obtained; the fermentation product 1 is mixed with a complex microbial solution 1 for anaerobic 
solid fermentation, and a fermentation product 2 is obtained; the fermentation product 2 is mixed with a complex microbial solution 2 for aerobic 
solid fermentation, and a fermentation product 3 is obtained; the fermentation product 3 is dried, smashed and sieved, and the microbial fermentation feed is obtained. By adopting 
solid-liquid combined fermentation and aerobic-anaerobic combined fermentation, the obtained feed contains rich 
cellulose nutrients, increases the food 
nutrient utilization rate and is high in 
protein content and beneficial to animal growth and development. By means of the microbial fermentation feed and the preparation method and application thereof, the breeding cost of the 
animal husbandry can be comprehensively reduced, and a large amount of grain for preparing 
livestock feed can be saved.