Microbial fermentation feed and preparation method and application thereof

A technology of microbial fermentation and compound microbial bacteria, applied in animal feed, animal feed, application, etc., can solve the problems of low cellulose degradation rate, low fermentation efficiency, poor process, etc., achieve high protein content, improve survival rate, Good for growth and development

Inactive Publication Date: 2017-01-18
曹蕊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the deficiencies and shortcomings of the prior art, such as backward strains, poor technology, low cellulose degradation rate, and low fermentation efficiency, the primary purpose of the present invention is to provide a method for preparing microbial fermented feed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Mix the plant-based material corn stalks and cakes (a mixture of soybean meal and rapeseed meal, the mass ratio is 1:1) according to the mass ratio of 5:1 and then pulverize, then add the compound enzyme preparation (cellulase, dextran enzyme, papain and pectinase) mixed evenly, and hydrolyzed under the conditions of pH 4.5 and temperature 35°C, wherein the solid-liquid ratio was 1:6, cellulase, dextranase, papain The consumption of pectinase and pectinase is respectively 400u / g plant base material, 200u / g plant base material, 200u / g plant base material, 200u / g plant base material; obtain enzymatic hydrolysis product;

[0059] (2) Mix the enzymatic hydrolyzate prepared in step (1) with the rumen content taken out from the rumen in the fresh cow stomach, wherein the mass ratio of the plant base material to the rumen content is 200:1; Anaerobic liquid fermentation for 12 hours under the condition of ℃, drying at 35 ℃, crushing and passing through a 60-mesh sieve to ob...

Embodiment 2

[0068] (1) The plant-based material corn stalks and cakes (a mixture of soybean meal and rapeseed meal, the mass ratio is 1:3) are mixed in a mass ratio of 2:1 and then pulverized, and then compound enzyme preparations (cellulase, dextran enzyme, papain and pectinase) were mixed evenly, and hydrolyzed at pH 4 and temperature 37°C, wherein the solid-liquid ratio was 1:5, cellulase, dextranase, papain The consumption of pectinase and pectinase is respectively 350u / g plant base material, 180u / g plant base material, 170u / g plant base material, 150u / g plant base material; obtain enzymatic hydrolysis product;

[0069] (2) Mix the enzymatic hydrolyzate prepared in step (1) with the rumen content taken out from the rumen in the fresh cow stomach, wherein the mass ratio of the plant base material to the rumen content is 150:1; Anaerobic liquid fermentation under the condition of 18h, dried at 45°C, crushed and passed through a 20-mesh sieve to obtain the fermented product 1;

[0070] ...

Embodiment 3

[0078] (1) The plant-based material corn stalks, cake meal (a mixture of soybean meal and rapeseed meal, the mass ratio is 1:2) and bran are mixed according to the mass ratio of 1:1:1 and then pulverized, and then the compound enzyme preparation (cellulose enzyme, dextranase, papain and pectinase) were mixed evenly, and hydrolyzed under the conditions of pH 5 and temperature 40°C, wherein the solid-liquid ratio was 1:3, cellulase, glucan The dosages of carbohydrase, papain and pectinase are 300u / g plant base material, 150u / g plant base material, 150u / g plant base material, 180u / g plant base material respectively; enzymatic hydrolysis products are obtained;

[0079] (2) Mix the enzymatic hydrolyzate prepared in step (1) with the rumen content taken out from the rumen in the fresh cow stomach, wherein the mass ratio of the plant base material to the rumen content is 100:1; Anaerobic liquid fermentation for 20 hours under the condition of ℃, drying at 37 ℃, crushing and passing t...

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Abstract

The invention belongs to the field of microbial fermentation, and particularly relates to a microbial fermentation feed and a preparation method and application thereof. The preparation method of the microbial fermentation feed includes the following steps that a plant base material is smashed, then a complex enzyme preparation is added and mixed for hydrolysis, and an enzymolysis product is obtained; the enzymolysis product is mixed with rumen content for anaerobic liquid fermentation, and a fermentation product 1 is obtained; the fermentation product 1 is mixed with a complex microbial solution 1 for anaerobic solid fermentation, and a fermentation product 2 is obtained; the fermentation product 2 is mixed with a complex microbial solution 2 for aerobic solid fermentation, and a fermentation product 3 is obtained; the fermentation product 3 is dried, smashed and sieved, and the microbial fermentation feed is obtained. By adopting solid-liquid combined fermentation and aerobic-anaerobic combined fermentation, the obtained feed contains rich cellulose nutrients, increases the food nutrient utilization rate and is high in protein content and beneficial to animal growth and development. By means of the microbial fermentation feed and the preparation method and application thereof, the breeding cost of the animal husbandry can be comprehensively reduced, and a large amount of grain for preparing livestock feed can be saved.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a microbial fermented feed and a preparation method and application thereof. Background technique [0002] People have been exploring how to solve the problem of low animal production. Until the 1950s, it was discovered that adding antibiotics to animal feed could significantly improve animal productivity. This discovery made a huge contribution to the intensification of animal husbandry. contribute. In recent years, the hazards of feeding feed containing antibiotics have received increasing attention from all walks of life. In 2004, the World Trade Organization, the Food and Agriculture Organization of the United Nations and the World Organization for Animal Health jointly held a symposium on the use of non-human antibiotics. Since January 2006, the European Union has completely banned the addition of antibiotics in livestock and poultry feed, and my country i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/30A23K10/37A23K10/26A23K10/14A23K10/18A23K50/30A23K50/75
CPCY02P60/87
Inventor 曹蕊
Owner 曹蕊
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