Method for regenerating rose into complete plant

Abstract

The invention discloses a method for regenerating a rose into a complete plant. The complete plant is prepared by obtaining a rose sterile seedling, inducing a somatic embryo, performing enrichment culture on the somatic embryo and germinating the somatic embryo into a seedling. In the method, the culture period is short; the survival rate is high; the proliferation multiple is high; the characteristics of a parent strain can be maintained completely; and the obtained somatic embryo is convenient to store for a long time and is an important receptor material for genetic transformation.

Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for regenerating a rose into a complete plant. Background technique

[0002] Rose (Rosa rugosa) is an upright deciduous shrub of the family Rosaceae (Rosaceae). It is native to northern my country. One of two types of flowers. Breeding new varieties of roses using the traditional cross-breeding method takes a long period of time and a lot of work, and will be limited by the variation of the breeding material itself, and the improvement range is limited, which is far from meeting the needs of the market. With the rapid development of biotechnology, plant cell engineering and genetic engineering technology are becoming more and more mature. On the basis of keeping other traits of varieties relatively stable, the traits controlled by foreign genes can be used for directional improvement, which improves the efficiency of breeding. . The establishment of the ro...

Examples

Embodiment

[0036] Test material selection, medium design and inoculation culture

[0037] 1. Source and treatment of test materials:

[0038] The test material rose 'Tangbai' among the present invention is collected from the flower test base of Huazhong Agricultural University, Wuhan City, Hubei Province. This variety was introduced in Pingyin County, Shandong Province. The strong young branches of the rose 'Tangbai' collected in the field are now rinsed with 1% washing powder for 10 minutes, then rinsed under tap water for 60 minutes, and then disinfected with 75% alcohol on the ultra-clean workbench for 30 seconds, and then disinfected with 1% mercury liter for 8 minutes , and finally wash 4-5 times with sterile water, cut the rose branches into 1-2cm size bud stem segments, inoculate them in the primary culture medium for primary culture, obtain sterile seedlings after one month of cultivation, and then inoculate the sterile seedlings Propagate and subculture on sterile seedling pro...

technical field

[0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for regenerating a rose into a complete plant. Background technique

[0002] Rose (Rosa rugosa) is an upright deciduous shrub of the family Rosaceae (Rosaceae). It is native to northern my country. One of two types of flowers. Breeding new varieties of roses using the traditional cross-breeding method takes a long period of time and a lot of work, and will be limited by the variation of the breeding material itself, and the improvement range is limited, which is far from meeting the needs of the market. With the rapid development of biotechnology, plant cell engineering and genetic engineering technology are becoming more and more mature. On the basis of keeping other traits of varieties relatively stable, the traits controlled by foreign genes can be used for directional improvement, which improves the efficiency of breeding. . The establishment of the ro...

Examples

Embodiment

[0036] Test material selection, medium design and inoculation culture

[0037] 1. Source and treatment of test materials:

[0038] The test material rose 'Tangbai' among the present invention is collected from the flower test base of Huazhong Agricultural University, Wuhan City, Hubei Province. This variety was introduced in Pingyin County, Shandong Province. The strong young branches of the rose 'Tangbai' collected in the field are now rinsed with 1% washing powder for 10 minutes, then rinsed under tap water for 60 minutes, and then disinfected with 75% alcohol on the ultra-clean workbench for 30 seconds, and then disinfected with 1% mercury liter for 8 minutes , and finally wash 4-5 times with sterile water, cut the rose branches into 1-2cm size bud stem segments, inoculate them in the primary culture medium for primary culture, obtain sterile seedlings after one month of cultivation, and then inoculate the sterile seedlings Propagate and subculture on sterile seedling pro...

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